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Leigh K. Hawkins, Fenny Dane, Thomas L. Kubisiak, Billy B. Rhodes, and Robert L. Jarret

Isozyme, randomly amplified polymorphic DNA (RAPD), and simple sequence repeats (SSR) markers were used to generate a linkage map in an F2 and F3 watermelon [Citrullus lanatus (Thumb.) Matsum. & Nakai] population derived from a cross between the fusarium wilt (Fusarium oxysporum f. sp. niveum) susceptible `New Hampshire Midget' and resistant PI 296341-FR. A 112.9 cM RAPD-based map consisting of 26 markers spanning two linkage groups was generated with F2 data. With F3 data, a 139 cM RAPD-based map consisting of 13 markers covering five linkage groups was constructed. Isozyme and SSR markers were unlinked. About 40% to 48% of the RAPD markers were significantly skewed from expected Mendelian segregation ratios in both generations. Bulked segregant analysis and single-factor analysis of variance were employed to identify RAPD markers linked to fusarium wilt caused by races 1 and 2 of F. oxysporum f. sp. niveum. Current linkage estimates between the resistance trait and the marker loci were too large for effective use in a marker-assisted selection program.

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Jian-Feng Geng, Cheng-Song Zhu, Xiao-Wei Zhang, Yan Cheng, Yuan-Ming Zhang, and Xi-Lin Hou

estimation of recombination fraction between consecutive markers and the order of distorted markers on the same linkage group ( Lorieux et al., 1995a , b ). Most quantitative geneticists who are interested in QTL mapping do not prefer to use distorted

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Vanessa E.T.M. Ashworth, Haofeng Chen, Carlos L. Calderón-Vázquez, Mary Lu Arpaia, David N. Kuhn, Mary L. Durbin, Livia Tommasini, Elizabeth Deyett, Zhenyu Jia, Michael T. Clegg, and Philippe E. Rolshausen

abundant genetic markers have declined significantly, and a shortage of markers no longer represents a constraint. We explore the possibility of generating a linkage map and of estimating QTLs for seven phenotypic traits collected in a mapping population of

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Yan Cheng, Qian Wang, Qingyu Ban, Jianfeng Geng, Xiao Wei Zhang, Ying Yi, and Xilin Hou

.P. 2007 The reference genetic linkage map for the multinational Brassica rapa genome sequencing project Theor. Appl. Genet. 115 777 792 Churchill, R.W. Doerge, G.A. 1994 Empirical threshold values for quantitative trait mapping Genetics 138 963 971 Chyi

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Yanbin Su, Yumei Liu, Huolin Shen, Xingguo Xiao, Zhansheng Li, Zhiyuan Fang, Limei Yang, Mu Zhuang, and Yangyong Zhang

-known cabbage QTLs for HSR on the generated linkage map and analyzed QTL stability across years with different mapping programs. Materials and Methods Plant materials. Two inbred cabbage lines ( Fig. 1 ), 96-100 and 79-156, were used as parents to develop a DH

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Eder J. Oliveira, Maria Lucia C. Vieira, Antonio Augusto F. Garcia, Carla F. Munhoz, Gabriel R.A. Margarido, Luciano Consoli, Frederico P. Matta, Michel C. Moraes, Maria I. Zucchi, and Maria Helena P. Fungaro

method of Wu et al. (2002) , which was used in our research. Therefore, we believe that integrated maps with good estimation of linkage phases provide a sound basis for QTL mapping and will provide the correct manipulation of favorable QRL alleles

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Jack E. Staub, Zhanyong Sun, Sang-Min Chung, and Richard L. Lower

Genetic linkage mapping has historically been the basis for genomic investigation and the analysis of quantitative trait loci (QTL) ( Doerge, 2002 ). The construction of a detailed linkage map is, in fact, the initial step for the use of genetic

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Amanda Garris, Lindsay Clark, Chris Owens, Steven McKay, James Luby, Kathy Mathiason, and Anne Fennell

Kosambi mapping function ( Kosambi, 1943 ). The use of SSR markers from previously published grape genome maps allowed for direct identification of the linkage groups according to the current Vitis chromosome nomenclature ( Doligez et al., 2006

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Bouchaib Khadari, Amal Zine El Aabidine, Cinderella Grout, Inès Ben Sadok, Agnès Doligez, Nathalie Moutier, Sylvain Santoni, and Evelyne Costes

olive using a large set of AFLP and SSR markers in a pseudo-test cross-mapping configuration. The population derived from a cross between the cultivars Olivière and Arbequina was chosen for establishing a genetic linkage map because they are genetically

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Yiqun Weng, Shanna Johnson, Jack E. Staub, and Sanwen Huang

, may be of limited value in MAS that seeks to change plant architecture. Therefore, a mapping project was designed to: 1) develop a moderately saturated linkage map using the Gy7 × H-19-derived RIL population; 2) identify SSR markers associated with ll