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Natalia R. Dolce, Luis A. Mroginski, and Hebe Y. Rey

Sansberro et al. (2000) who have found that low-temperature treatment increased the germination percentage of I. paraguariensis isolated embryos and cut pyrenes cultured in vitro. In many species, exposition of seeds to low temperatures decreases the

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Song-jun Zeng, Zhi-lin Chen, Kun-lin Wu, Jian-xia Zhang, Cheng-ke Bai, Jaime A. Teixeira da Silva, and Jun Duan

. Asymbiotic seed germination and in vitro seedling development of Nothodoritis zhejiangensis . ( A ) Flowering specimen of N. zhejiangens growing on the tree branches of Cornus officinalis . ( B ) Seed germination and protocorm development in vitro. ( C

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Ernie DeMarie, Molly Weimer, and K.W. Mudge

Green pods of the C. reginae orchid were collected from a bog near Ithaca, NY. Pods were surface sterilized, and seeds were plated on agar media. The 8 germination treatments were arrange in a complete 3 way factorial consisting of 2 basal media (fish emulsion, FE vs. yeast extract, YE), 2 media pHs (4.8, 6.8), and 2 temperature regimes (constant 24 C vs. 6 wks. at 5 C prior to transfer to 24 C). Sequential stages of development included embryo enlargement and rupture of the testa (l), root elongation (2), leaf primordium development (3) and finally rhizoid development with or without protocorm greening (4). After 4 months post sowing, germination (stage 1 or beyond) was 56% and 0% on FE at pH 4.8 and 6.8 respectively, and 45% and 78% on YE at pH 4.8 and 6.8 respectively. Protocorm development from unchilled seeds after 4 months was greatest on YE at the lower pH, with 14% reaching stage 3 or 4, as contrasted to only 5% reaching stage 2 (none beyond), for the other germinated treatments. Chilled seeds had higher germination for all treatments but no development beyond stage 1 at 4 months post sowing.

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M.A.R. Mian, R.M. Skirvin, M.A. Norton, and A.G. Otterbacher

., Baton Rouge, LA 70803. 4 Principal Research Specialist in Horticulture. Funds for this research were provided in part by the Illinois Agricultural Experiment Station. Special thanks to Gene Galletta and Adam Dale for their encouragement in this

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A. Raymond Miller, Joseph C. Scheereus, Patricia S. Erb, and Craig K. Chandler

1 To whom reprint requests should be addressed. Journal Article no. 249-90. Salaries and research support provided in part by state and federal funds appropriated to the Ohio Agricultural Research and Development Center and a Seed Grant to A.R. M

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Stephen Patrick Greer and Timothy A. Rinehart

. paniculata and there are no published reports as to the relative effectiveness of this mutagen in these species. In addition, nothing is known about the effects of EMS on the viability, dormancy, or germination of Hydrangea seeds. We performed a series of

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Takahiro Tezuka, Hisa Yokoyama, Hideyuki Tanaka, Shuji Shiozaki, and Masayuki Oda

. latifolia and I. rotunda were sown on wet filter paper, germination was not observed in either the 2006–07 or 2007 seasons. In contrast, when cut seeds or embryos were cultured in vitro, quarter-seeds without endocarp (sown on 20 July 2007) and embryos of

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Xiuli Shen and Myeong-Je Cho

factors governing dormancy and germination in sugar pine. Ultimately, the objective of this study was to develop a reliable and rapid in vitro germination protocol in sugar pine. To achieve this goal, we sought to 1) examine seed morphology and structure

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Arancha Arbeloa, Ma Elena Daorden, Elena García, Pilar Andreu, and Juan A. Marín

Effects of media on embryo enlargement, germination and plant development in early-ripening genotypes of Prunus grown in vitro Plant Cell Tiss. and Org. Cult. 37 55 59 García-Gusano, M. Martínez-Gómez, P. Dicenta, F. 2004 Breaking seed dormancy in almond

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Natalia R. Dolce, Ricardo D. Medina, and María T. González-Arnao

of seven South American Ilex species and comparing different methodologies for in vitro germination of fresh and cryostored seeds, including I. paraguariensis (the source of raw material for the production of maté, the most popular beverage in the