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Alexander D. Pavlista, Dipak K. Santra, James A. Schild, and Gary W. Hergert

In dry bean ( Phaseolus vulgaris L.), growth form, i.e., determinate vs. indeterminate, and growth habit, i.e., upright/erect/bushy vs. viny/prostate, are among the most important characteristics for classifying cultivars from an agronomic

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Neil O. Anderson, Peter D. Ascher, and Emily E. Hoover

Two-species CBC hybrids between Phaseolus vulgaris and P. acutifolius exhibit transgressive segregation for seed color and patterning, root peroxidases, and seed proteins. CBC pedigrees between P. vulgaris and P. coccineus (differing for species-specific traits) were created to test whether variation would be similar or greater than with P. acutifolius. P. vulgaris `Soldier' (Vermont) × 2- and 4-way intraspecific P. coccineus accessions were used as parents. CBC1 through CBC3 were evaluated for segregation of species-specific genes. Hybrid breakdown was evident in all CBC generations, particularly nonflowering dwarf cripples. Transgressive segregants were found as early as CBC2. One individual was found that had crossovers for species traits: a determinate, red-flowered plant with P. coccineus flowers and P. vulgaris introrse stigmas. By CBC3, all of the variation reported for three-species CBC hybrids (P. coccineus × [P. vulgaris × P. acutifolius]) was evident.

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J. Nienhuis, P. Skroch, M. Sass, S. Beebe, J. Tohme, and F. Pedraza

The number of Phaseolus vulgaris germplasm accessions numbers more than 30,000. While the large numbers of accessions increase the probability of preserving genetic variability they simultaneously limit the efficient and routine utilization of this resource. From the approximately 4000 P. vulgaris accessions in the C.I.A.T. whole collection that were collected in Mexico, a core collection of 400 accessions was developed based on variation for agronomic performance, ecological adaptation, and seed characteristics. Random samples of 90 accessions each were drawn from the core and whole collections and evaluated for 224 polymorphic RAPD bands. Based on analysis of the RAPD data there were no significant differences in genetic diversity between the two samples. The correlation of marker frequency for the two samples was 0.984 confirming that the two samples represent the same population.

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B.A. Mullin, G.S. Abawi, M.A. Pastor-Corrales, and J.L. Kornegay

A stem grafting technique was used to determine the contribution of root and shoot tissues of bean (Phaseolus vulgaris L.) to the resistance response to the root-knot nematode, Meloidogyne incognita (Kofoid and White, 1919) Chitwood 1949. Stemgrafts were prepared between resistant (line A 211 or cultivar Nemasnap) and susceptible (Canario Divex) bean cultivars in all possible scion-rootstock combinations. Graft combinations in which the rootstock was resistant resulted in a resistant response to M. incognita, and those combinations in which the rootstock was susceptible resulted in a susceptible response, regardless of scion component. Resistance factors were therefore either localized within roots or not translocated basipetally through the stem graft union.

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E. Arnaud-Santana, M.T. Mmbaga, D.P. Coyne, and J.R. Steadman

We studied leaf and pod reactions of 18 Phaseolus vulgaris germplasm lines (three temperate and 15 tropical) to four Xanthomonas campestris pv. phaseoli (XCP) (Smith) Dye strains and seven Uromyces appendiculatus (UA) (Pers.) Unger races. Line × XCP interaction was significant for leaf and pod reactions. The common bean lines XAN-159, BAC-6, and XAN-112 had the best combined leaf and pod resistance to XCP. Line × UA race interactions were significant (P = 0.05). Lines IAPAR-14 and BAC-6 had the best combined resistance to XCP and UA.

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R. Provvidenti

Passionfruit woodiness virus (PWV) can infect bean (Phaseolus vulgaris L.), causing a light and dark green foliar mosaic, veinbanding, downward curling, and plant stunting. The intensity of these symptoms can vary with the strain of the virus and cultivar, but they resemble those caused by bean common mosaic virus. In genetic populations derived from crosses and backcrosses involving cultivars that are resistant (`Black Turtle 1', `Clipper', and `RedKote') or susceptible (`Black Turtle 2', `California Light Red Kidney', and `Pioneer'), a single dominant gene conferred resistance to an Australian strain PWV-K. To this gene, the symbol Pwv (Passionfruit woodiness virus) is tentatively assigned. In plants derived from rooted cuttings of backcross populations, the same factor also conditioned resistance to three other Australian strains, PWV-Mild, PWV-51, and PWV-Tip Blight.

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James Nienhuis, Paul Skroch, and Steve Beebe

Nuñas are a type of common bean (Phaseolus vulgaris) that possess the unusual characteristic of popping or expanding their cotyledonary tissue when heated. Numerous landraces of nuña beans were domesticated in the Andean region of South America (Peru, Bolivia, and Ecuador) and have been grown and consumed in this region since antiquity. The practical consideration in the domestication of nuñas in the high Andes was likely due to the greater energy efficiency in cooking toasted vs. boiled seeds.The Phaseolus germplasm bank at CIAT (Centro Internacional de Agricultura Tropical) has developed a core collection of Andean beans that includes numerous nuña landraces. Based on the wide range of phaseolin types observed among nuña landraces, it has been hypothesized that nuñas may represent a greater source of genetic diversity compared to other landraces and cultivars of common bean. Eighty nuña accessions and 120 nonpopping common bean accessions were randomly sampled from the CIAT Andean germplasm core collection. The 200 accessions were characterized for 140 mapped RAPD markers. The objectives of our research were to 1) understand the genetic structure of nuña bean accessions relative to other Andean common beans, and 2) to measure the genetic distance and genetic diversity between nuña and other Andean bean populations.

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Dyremple B. Marsh, Wayne McLaughlin, and James S. Beaver

Methods to improve the grain yield of red kidney bean without the addition of commercially fixed nitrogen will have significant benefits to farmers in Jamaica and other tropical regions. Red kidney beans provide a major portion of the dietary protein for most families in these regions. Our experimental objective was to evaluate the nitrogen fixing capabilities of several breeding lines of Phaseolus vulgaris when inoculated with Rhizobium strains isolated from Jamaican soils. Surface sterilized seeds of 11 Phaseolus lines were inoculated with inoculum prepared from 5 day old Rhizobium YEM mixture. Rhizobium used were T2 and B17 from Jamaica and UMR 1889. The greenhouse study was arranged as a completely randomized design. Bean lines 9056-101, 9056-98B, 8954-5 and 8954-4 showed improved nodulation and N2 fixation when inoculated with UMR 1899. The combination of breeding line 8954-5 and Rhizobium strain B17 produced the highest nodule number and shoot dry weight of 193 and 0.72 g, respectively. The Rhizobium strain B17showed some ability to compete successfully for nodule sites against known effective strains.

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R.S. Balardin and J.D. Kelly

Sixty-two genetically diverse modern and traditional Phaseolus vulgaris L. cultivars from Brazil, the Dominican Republic, Honduras, Mexico, the Netherlands, and the United States, representative of the Andean and Middle American gene pools, were selected to study the interaction with distinct races of Colletotrichum lindemuthianum (Sacc. & Magnus) Lams.-Scrib. Principal component and phenetic analyses were conducted on the disease reaction to inoculation with 34 races of C. lindemuthianum from Argentina, Brazil, Colombia, Costa Rica, the Dominican Republic, Honduras, Mexico, Peru, and the United States. The principal component analysis revealed four clusters in which only one cluster consisted of cultivars from both gene pools. Bean genotypes clustered based on the gene pool origin of the resistance genes present, regardless of the actual gene pool of the host genotype. Middle American genotypes in cluster A carried Andean resistance genes. Further grouping of genotypes based on overall level of resistance within each gene pool was observed. Clusters A and C consisted of the most resistant genotypes from both gene pools. The distribution of genotypes generated by the phenetic analysis, placed the most resistant and susceptible genotypes of the anthracnose differential series at the extremities of the phenogram, providing support for the range in genotypic resistance exhibited by members of the differential series. Races of C. lindemuthianum isolated from Middle American genotypes showed broad virulence on germplasm from both gene pools, whereas races with Andean reaction showed high virulence only on Andean germplasm. The reduced virulence of Andean races on Middle American genotypes suggests selection of virulence factors congruent with diversity in P. vulgaris. In addition, races of C. lindemuthianum formed two clusters corresponding to the Middle American and Andean reaction groups based on the phenetic analysis. In the principal component analysis, most races with the Andean reaction were observed in the clusters C and D, except races 15 and 23 which clustered with Middle American races in cluster B. Only races 38, 39 and 47 from the Dominican Republic showed high similarity in both multivariate analyses and clustered based on geographic origin. Races from other countries showed no geographic effect. The overlapping of specific races, however, with races from different reaction groups might indicate that this group of isolates possesses factors of virulence to both host gene pools. Data based on virulence supports variability in C. lindemuthianum structured with diversity in P. vulgaris.

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Paige Hanning, Dyremple B. Marsh, and Mohsen Dkhili

Chemically fixed nitrogen is a costly import for Caribbean Basin Countries. Increased cost of fertilizer only serves to reduce crop yields in these areas. This greenhouse research was undertaken to evaluate the N2 fixing capabilities and yield potential of several Phaseolus vulgaris lines developed for use in Caribbean Basin countries. Ten common bean lines from breeding programs at the Universities of Puerto Rico and Wisconsin and two efficient Rhizobium phaseoli strains were used for the study. Plants treated with Rhizobium UMR 1899 and UMR 1632 had significantly higher stem and leaf dry weight than the control plants. Bean lines WBR 22-34, WBR 22-50, WBR 22-55, PR9056-98B and the cultivar Coxstone showed increased dry matter accumulation over that of the control plants. Plants treated with the Rhizobium strain UMR 1899 had the highest stem and leaf dry matter accumulation. Nodulation was significantly higher when plants were treated with UMR 1632. The lines WBR 22-34 and PR 9056-98B produced more nodules than the other lines used. Pod yield as measured by number of immature pods was highest for PR 9056-98B when inoculated with Rhizobium UMR 1899.