preparation and maintenance of the protoplast and tissue cultures and Assaf Guri of Plant Cell Technology for generously providing PPM samples for testing.
Thomas W. Zimmerman and Jacqueline Kowalski
43 POSTER SESSION 6 (Abstr. 307-325) Propagation/Tissue Culture Monday, 24 July, 1:00-2:00 p.m.
Jeanne M. Grout and Paul E. Read
The propagation method and vegetative condition of ‘Northblue’ blueberry (Vaccinium corymbosum L.) stock plants influenced microshoot production in vitro and root formation on leaf-bud cuttings. In tissue culture (TC), explants from TC-derived stock plants produced longer shoots than explants from leaf-bud, standard- (ST-) derived stock on either Zimmerman’s (Zimm) medium at pH 4.8, Lloyd and McCown’s woody plant medium (WPM) at pH 4.8 or pH 5.2. Microshoots from explants of TC-stock plants also rooted more readily. Microshoot rootability decreased after 18 weeks on medium containing 68.6 μmol (12 mg/liter) 2iP. Microshoot production and rootability increased after 3 additional weeks on Zimm medium without 2iP present. Leaf-bud cuttings of ‘Northblue’ TC-stock plants treated with 5% and 10% concentrations of a commercial rooting compound (Dip-n-Grow) had a slightly higher rooting percentage and root rating than nontreated cuttings from ST-stock plants. However, cuttings from ST-stock plants of the same age showed larger increases in root formation and percentage of rooting in response to the same rooting compound treatments. Leaf-bud cuttings from vegetative TC-stock plants that developed shoots had more basal branches than those from floral ST-stock plants. Branch elongation was greatest and basal branches fewest on cuttings from floral ST stock plants. Successful propagation with cuttings and in vitro explants may be related to the condition of the stock plants, which have been altered by their own propagation methods and the plant growth regulators applied. Chemical names used: 1H-indole-3-acetic acid (IAA); 1H-indole-3-butanoic acid (IBA); 1-naphthaleneacetic acid (NAA); N-(3-methyl-2-butenyl)-2H-purin-6-amine (2iP).
Brent Tisserat and Robert Silman
93 ORAL SESSION 19 (Abstr. 129–135) Cell and Tissue Culture
Miles Schwartz Sax, Nina Bassuk, and Mark Bridgen
stool-bed rooting method. To overcome this limitation, tissue culture protocols were trialed to determine if in vitro methods could successfully be used to clonally propagate UHI hybrid oaks. The use of oak tissue culture methods used to grow plants
Carol A. Bobisud, Susan P. Martin, and Terry T. Sekioka
177 POSTER SESSION 25 (Abstr. 870-901) Cell and Tissue Culture/Propagation
M. Keskitalo, A. Pohto, M.L. Savela, J.P.T. Valkonen, E. Pehu, and J. Simon
147 POSTER SESSION (Abstr. 375–388) Cell and Tissue Culture II
Fucheng Shan and Kevin Seaton
technique was the elimination of the need for explants’ initiation and in vitro maintenance as required by micropropagation tissue culture protocols and the need for multinodal cuttings by use of single fresh nodes as compared with ex vivo cuttings
Seong Min Woo and Hazel Y. Wetzstein
that Georgia plume's seed dormancy mechanism required a prolonged chilling period (42 to 64 d) for germination. Propagation by root cutting methods can produce shoots, but in a limited number. Tissue culture methods can be an excellent option for the
Toktam Taghavi, Alireza Rahemi, Reza Rafie, and Maru K. Kering
fungus also can survive in diseased rhizomes kept for planting. The slow multiplication rate (annually 5- to 10-fold) has limited the availability of high-yielding genotypes. Tissue culture techniques allow for rapid clonal propagation of turmeric