preparation and maintenance of the protoplast and tissue cultures and Assaf Guri of Plant Cell Technology for generously providing PPM samples for testing.
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Randall P. Niedz
Thomas W. Zimmerman and Jacqueline Kowalski
43 POSTER SESSION 6 (Abstr. 307-325) Propagation/Tissue Culture Monday, 24 July, 1:00-2:00 p.m.
Brent Tisserat and Robert Silman
93 ORAL SESSION 19 (Abstr. 129–135) Cell and Tissue Culture
Miles Schwartz Sax, Nina Bassuk, and Mark Bridgen
stool-bed rooting method. To overcome this limitation, tissue culture protocols were trialed to determine if in vitro methods could successfully be used to clonally propagate UHI hybrid oaks. The use of oak tissue culture methods used to grow plants
Carol A. Bobisud, Susan P. Martin, and Terry T. Sekioka
177 POSTER SESSION 25 (Abstr. 870-901) Cell and Tissue Culture/Propagation
M. Keskitalo, A. Pohto, M.L. Savela, J.P.T. Valkonen, E. Pehu, and J. Simon
147 POSTER SESSION (Abstr. 375–388) Cell and Tissue Culture II
Fucheng Shan and Kevin Seaton
technique was the elimination of the need for explants’ initiation and in vitro maintenance as required by micropropagation tissue culture protocols and the need for multinodal cuttings by use of single fresh nodes as compared with ex vivo cuttings
Seong Min Woo and Hazel Y. Wetzstein
that Georgia plume's seed dormancy mechanism required a prolonged chilling period (42 to 64 d) for germination. Propagation by root cutting methods can produce shoots, but in a limited number. Tissue culture methods can be an excellent option for the
Davut Keleş, Ceren Özcan, Hasan Pınar, Atilla Ata, Nihal Denli, Namık Kemal Yücel, Hatıra Taşkın, and Saadet Büyükalaca
, haploidization offers a great advantage by shortening the breeding cycle. Haploid plants can be obtained using various tissue culture techniques and full homozygosity can be achieved in quite a short time using these methods. Since the heterozygosity rate is high
Lurline Marsh and Moshen Dkhill
Tissue culture of four cowpea (Vigna unguiculata) and two pigeonpea (Cajanus cajan) genotypes was tested on Blaydes' medium supplemented with different hormone concentrations. Explants of cotyledonary nodes, cotyledons and leaves of the cowpea genotype IT82E-16, IT64E-124, Pinkeye Purple Hull and MN13 produced callus after 4 weeks in Blaydes' medium. The hormone combinations in the medium were 2-l dichlorophenoxy acetic acid (2.4-D) (2 mg/liter) and kinetin at 0.05, 0.1, 0.5 mg/liter, or 2,4-D and thidiazuron (TDZ) at 2.2, 4.4 and 6.6 mg/liter or benzylaminopurine (BA) at 2.25, 4.5 or 6.75 mg/liter. Shoots occured on cotyledonary nodes of Pinkeye Purple Hull In the TDZ (6.6 mg/liter). Roots were produced from the leaf and cotyledonary nodes of Pinkeye Purple Hull and on cotyledons of IT-64E-124 cultured In media with kinetin (0.5 mg/liter). Leaf and cotyledon explants of pigeonpea genotype; ICPL 146 1965HK and ICPL 65024 produced callus and some shoots in BA (2.25 mg/liter) after 4 weeks. The callus when subcultured on BA (0.5 mg/liter) and NAA (0.1 mg/liter) produced shoots. Regenerated shoots rooted in the Blaydes' medium with kinetin (0.01 mg/liter) and NAA (0.6 mg/liter).