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Thomas O. Athoo, Andreas Winkler, and Moritz Knoche

analysis (Cell P ; Olympus Europa, Hamburg, Germany). To establish the distribution of stomata along the pedicel, ‘Sam’ pedicels were cut into five sections each ≈8 mm in length, which were then cut along their long axes into two halves and incubated in 50

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Rashad M. Balal, Muhammad M. Khan, Muhammad A. Shahid, Neil S. Mattson, Tahira Abbas, Muhammad Ashfaq, Franscisco Garcia-Sanchez, Usman Ghazanfer, Vicente Gimeno, and Zafar Iqbal

micrometer (MeCan, Saitama, Japan), whereas the leaf was viewed on a computer monitor through the use of EFD-3 software (Shenzhen Eternal Science, Beijing, China). Determination of number of stomata, epidermal cells, and stomatal size The number of stomata

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Kenneth R. Schroeder and Dennis P. Stimart

Leaf impressions were made from two short-lived (4 and 5 d) inbreds, a long-lived (11 d) inbred, and their hybrids (8 and 9 d) of Antirrhinum majus L. using Super Glue and glass microscope slides. Leaves were taken from mid stem, pressed on glass slides (under side down), spread with a small amount of Super Glue, set for 3 to 4 s. Then, the leaf was peeled off leaving a permanent impression in the glue. Slides were placed under a microscope equipped with a video imaging system and computer images were taken to facilitate counting of stomatal complexes. Number of stomata ranged from 10,400 to 21,300 per cm2 of leaf. A LI-COR LI-3100 area meter (LI-COR, Inc. Lincoln, Neb.) was used to measure total leaf area of 40-cm cut flower stems of each accession. Stomata per flowering stem ranged from 1,074,000 to 2,282,000, with the long-lived inbred having the fewest stomata, the hybrids intermediate with 11% to 21% more, and the short-lived inbreds having 40% to 113% more stomata per stem. It appears long postharvest life of A. majus is associated with flowering stems with fewer stomata per cut stem.

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Moritz Knoche and Stefanie Peschel

133 DAFB) were associated with stomata. Typically, microcracks occurred first in the CM above the common periclinal cell wall of a pair of guard cells ( Fig. 5A ), subsequently extended along the long axis of the aperture of the stomatal apparatus

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Li-ping Chen, Yan-ju Wang, and Man Zhao

In this study, in vitro induction of tetraploid Lychnis senno Siebold et Zucc. and its cytological and morphological characterization were conducted. For polyploid induction, nodal segments with axillary buds from in vitro grown plants were kept for 3 days in MS (Murashige and Skoog, 1962) liquid or solid media added with a series of concentrations of colchicine. Out of total 588 recovered plants, 15 tetraploids and 6 mixoploids determined by flow cytometry analysis were obtained. The tetraploid contained 48 chromosomes, twice the normal diploid number of 24, as observed under light microscope. The tetraploid plants exhibited much larger but less stomata than diploid plants. Moreover, significant differences in stem height and leaf size between the diploid and tetraploid plants were noted. The tetraploid plants were more compact than diploids.

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M.E. El-Mahrouk, A.R. El-Shereif, Y.H. Dewir, Y.M. Hafez, Kh. A. Abdelaal, S. El-Hendawy, H. Migdadi, and R.S. Al-Obeed

of hyperhydricity on thylakoid membranes ( Chakrabarty et al., 2006 ; Marschner and Possingham, 1975 ). The malformed nonfunctional stomata is a common abnormality in hyperhydric shoots ( Apóstolo and Llorente, 2000 ; Barbosa et al., 2013 ; Gribble

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Geoffrey M. Weaver and Marc W. van Iersel

abscisic acid improved the postharvest drought tolerance of several annual bedding plants Acta Hort. 755 127 132 Buckley, T.N. 2005 The control of stomata by water balance New Phytol. 168 275 292 Chapman, S.C. Barreto, H.J. 1997 Using a chlorophyll meter to

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Kenneth R. Schroeder and Dennis P. Stimart

Evaluation of leaf stomatal numbers and postharvest water loss indicate these are important factors in Antirrhinum majus (snapdragon) cut flower postharvest longevity (PHL). Cut flowers with 9 days longer PHL had 53% fewer leaf stomata. Long PHL is associated with an early reduction in transpiration followed by low steady transpiration. Short-lived genotypes had a linear transpiration pattern over the period of PHL indicating poor stomatal control of water loss. Short-lived genotypes had 22% to 33% reductions in fourth quarter transpiration while long-lived genotypes had 2% to 8% reductions. In addition, short-lived genotypes had higher average fourth quarter cut flower weight losses compared to long-lived genotypes. Further investigation of stomatal numbers and functioning relative to PHL may provide breeders a rapid and nondestructive indirect selection method for PHL.

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Seenivasan Natarajan and Jeff S. Kuehny

photosynthetic photon flux of 500 μmol·m −2 ·s −1 on recently matured leaves of 8-week-old plants; two measurements were recorded per plant on separate leaves. Stomata size, stomatal number, and leaf thickness. Two recently matured leaf samples from each

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Holger Weichert, Stefanie Peschel, Moritz Knoche, and Dieter Neumann

-transport from a 10 m m FeCl 3 donor solution (pH 2.6) was established in a two-phase experiment using astomatous ‘Adriana’ and stomatous ‘Sam’ ES. Stomata number was established by microscopy on an individual ES basis. ‘Adriana’ sweet cherry has a very low