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Bruce D. Whitaker

MG tomato fruit were stored for four or 12 days at chilling (2C) or nonchilling (15C) temperature. Fruits stored 12 days at 15C ripened to the turning stage, whereas fruits at 2C did not ripen. Lipids of microsomes and plastids from pericarp tissue were analyzed at harvest and after four or 12 days of storage. After 12 days at either 15C or 2C, the ratio of phospholipid (PL) to protein in microsomes declined, with a concomitant increase in the ratio of total membrane sterols (TMS) to PL. The TMS/PL ratio also increased in crude plastids. In both microsomes and plastids, free sterols (FS) increased more at 2C than at 15C, and thus accounted for a larger percentage of the TMS. The ratio of stigmasterol to sitosterol in steryl lipids, particularly in FS, increased more at 15C than at 2C. The unsaturation index of fatty acids in PL and galactolipids generally increased slightly during storage at both 15C and 2C. The ratio of phosphatidylethanolamine to P-choline increased in both membrane fractions at both temperatures. In plastids, the ratio of mono- to digalactosyldiacylglycerol declined substantially at 2C but not at 15C.

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Qi Zhang, Jack Fry, Channa Rajashekar, Dale Bremer, and Milton Engelke

galactolipid analysis. Phospholipid and galactolipid standards were purchased from Avanti Polar Lipids (Alabaster, AL) and Matreya (State College, PA), respectively, except di24:1-PE and di24:1-PG, which were prepared by transphosphatidylation of di24:1-PC

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Charles Fontanier, Justin Quetone Moss, Lakshmy Gopinath, Carla Goad, Kemin Su, and Yanqi Wu

Salisbury, 1973 ). The thylakoid membrane system is primarily composed of galactolipids: monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) ( Douce and Joyard, 1990 ). In contrast, the plasma membrane primarily consists of

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David R. Rudell, Sara Serra, Nathanael Sullivan, James P. Mattheis, and Stefano Musacchi

closely related species. Galactolipids (MGDGs and DGDGs) are typically principal components of the thylakoid membrane ( Robinson and Mant, 2009 ). Plastiglobules that form during chromoplastogenesis, a process characterized by degreening, contain very

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Shirin Shahkoomahally and Asghar Ramezanian

inhibit the development of maleness Acta Hort. 682 1171 1178 Picchioni, G. Watada, A. Conway, W. Whitaker, B. Sams, C. 1995 Phospholipid, galactolipid, and steryl lipid composition of apple fruit cortical tissue following postharvest CaCl 2 infiltration

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Andrés Olivos, Scott Johnson, Qin Xiaoqiong, and Carlos H. Crisosto

as cold temperature storage, may trigger this situation. Plants can replace membrane phospholipids with galactolipids (non-phosphorous lipids) in response to P-limited conditions ( Tjellstrom et al., 2008 ). However, it is not clear how this

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Kemin Su, Dale J. Bremer, Richard Jeannotte, Ruth Welti, and Celeste Yang

of the Arabidopsis thermosensitive mutant atts02 reveals an important role for galactolipids in thermotolerance Plant Cell Environ. 29 1437 1448 Cullis, P.R. Dekruijff, B. 1979 Lipid polymorphism and the