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Cristian Silvestri, Gianmarco Sabbatini, Federico Marangelli, Eddo Rugini, and Valerio Cristofori

not easy to root and acclimatize, the rooting phase has been successfully performed ex vitro and the plants then survived when planted in the field ( Benmahioul et al., 2012 ; Saiju, 2005 ). The present work has been conducted to develop an efficient

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Mohammed Elsayed El-Mahrouk, Yaser Hassan Dewir, and Yougasphree Naidoo

different concentrations of IBA or NAA at 0, 1, 3, and 5 mg·L −1 . Culture conditions. The cultures were incubated at 25 ± 2 °C under a 16-h photoperiod provided by cool-white fluorescent tubes at 35 μmol·m −2 ·s −1 PPFD. Acclimatization. Plantlets at the

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Yin-Tung Wang

objectives of this study were to 1) determine the impact of a range of temperature and duration during simulated shipping and 2) characterize the effect of thermal acclimatizing plants before simulated shipping on the morphology and subsequent greenhouse

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Chao Dong, Xue Li, Yue Xi, and Zong-Ming Cheng

, with 16/8 h light/dark (the light intensity is 100 μmol·m −2 ·s −1 ), and the relative humidity was 70% ± 5%. After culturing for 1 month, the number of rooted microcuttings and the number of roots per microcutting were determined. Acclimatization and

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Meltem Bayraktar

acclimatization performance of plantlets obtained from semisolid and RITA ® bioreactors also was evaluated. Materials and Methods Plant material. In vitro–propagated plantlets, which were obtained from single seed descent seedlings possessing the best

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Ana V. de Souza, José E.B.P. Pinto, Suzan K.V. Bertolucci, Ricardo M. Corrêa, Larissa C. do B. Costa, and William E. Dyer

micropropagation using nodal segments as well as plantlet development in different media for greenhouse acclimatization and field establishment. Materials and Methods Achenes from field L. pinaster plants were cleaned under air-flow shrunken (95%) and were

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Hilda E. Lee-Espinosa, Joaquín Murguía-González, Benjamín García-Rosas, Ana L. Córdova-Contreras, Antonio Laguna-Cerda, Javier O. Mijangos-Cortés, Luis F. Barahona-Pérez, Lourdes G. Iglesias-Andreu, and Nancy Santana-Buzzy

-scale micropropagation protocol for this species through the optimal use of axillary buds from each mother plant to achieve the highest number of adventitious shoots per bud per plant and to promote successful acclimatization of the regenerated plants. Materials and

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Aekaterini N. Martini, Maria Papafotiou, and Stavros N. Vemmos

location on shoots (apical, axillary), and season of explant collection on contamination and explant browning as well as on all stages of in vitro propagation of × M. florentina , including microshoot rooting and acclimatization. The phenolic content of

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Veronica T. Lewandowski

Routine methods were developed to propagate through tissue culture large numbers of Vitis labrusca L. `Delaware' cuttings on a 10-week production cycle. A 35- fold increase in shoots was achieved in 4 weeks through axillary bud proliferation of rejuvenated cultures using a modified Murashige and Skoog (MS) medium. Additive root-stimulating effects from the auxin combination of NAA at 0.001 mg·1iter–1 and IBA at 0.005 mg·liter–1 in half-strength MS resulted in > 95% rooting of microcuttings in 10 days. More than 95% of the rooted plantlets were successfully acclimatized within 14 days using greenhouse flats fitted with humidity domes and containing a fortified soilless growing medium. Leaves from 21-day-old greenhouse plantlets lost 21% water after 40 min of holding at 40% relative humidity, compared to 62% from 9-day-old plantlets and 91% from 3-day-old plantlets. The combined features reported here enable clonal production of ≈ 3000 `Delaware' grape plants per month using simplified methods and reduced intervals between sequential stages of development. Chemical names used: α-naphthaleneacetic acid (NAA), 1H-indole-3-butyric acid (IBA).

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James R. Ault

Shoot formation was obtained from Lachenalia arbuthnotiae W.F. Barker, L. bulbifera (Cyrillo) Engl., and L. purpureo-coerulea Jacq. leaf tissue explants cultured on Murashige and Skoog (MS) medium supplemented with sucrose at 30 g·liter–1, 8.87 μm BA, and 0.44 μm K-NAA. Shoots of all three species rooted on subculture to MS medium supplemented with 0.0, 4.14, or 8.29 μm K-IBA or 0.0, 4.46, or 8.92 μm K-NAA. Maximum percent rooting was ≈81% from treatment with 4.14 μm K-IBA for L. arbuthnotiae and with 8.29 μm K-IBA for L. purpureo-coerulea; it was 59% from treatment with 8.92 μm K-NAA for L. bulbifera. Rooted and nonrooted shoots were acclimatized in a greenhouse. Survival of rooted plants was 93% for L. arbuthnotiae, 95% for L. bulbifera, and 94% for L. purpureo-coerulea. Survival of nonrooted shoots was 71% for L. arbuthnotiae and 91% for L. bulbifera. Chemical names used: 6-benzyladenine (BA); potassium salt of indole-3-butyric acid (K-IBA); potassium salt of 1-naphthaleneacetic acid (K-NAA).