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J. Kevin Parris, Darren H. Touchell, Thomas G. Ranney, and Jeffrey Adelberg

·L −1 2-(N-morpholino) ethanesulfonic acid (MES) monohydrate, and 30 g·L −1 sucrose. Media were adjusted to a pH of 5.75 ± 0.03 and solidified with 0.8% agar (Phytotech Laboratories). Media (25 mL) was dispensed to 180-mL glass jars. Proliferated

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Kelly M. Oates, Darren H. Touchell, and Thomas G. Ranney

( Murashige and Skoog, 1962 ) supplemented with 0.1 g·L −1 myoinositol, 0.1 g·L −1 2-(N-Morpholino) ethanesulfonic acid (MES) monohydrate, 5 μM 6-benzylaminopurine (BAP), 0.625 μM 1-napthyl acetic acid, 30 g·L −1 sucrose, pH adjusted to 5.75 ± 0.03, and

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Chia-Yun Ko, Tsai-Yun Lin, Chin-Wen Ho, and Jei-Fu Shaw

HCl (10 mg·L −1 ), sucrose (20 g·L −1 ), and 2-(N-morpholino)ethanesulfonic acid (MES) (500 mg·L −1 ). The medium contained MES to maintain a stable pH. Each sample of 2.0 g explant from the primary culture was subcultured in 30 mL one-tenth MLMS

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Jeffrey A. Anderson

-week-old plants and rinsed in deionized water and then gently blotted free of surface moisture. The midribs were removed with a razor blade and the leaves were cut into sections. Leaf extracts were prepared at 62.5 mg FW per milliliter of 2-(N-morpholino

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Christopher C. Dickinson, Alexandra J. Weisberg, and John G. Jelesko

. The culture optical density (OD) at 600 nm (OD 600 ) was estimated from a 1:10 culture dilution before initial centrifugation (15 min at 4 °C, 3000 × g ). Cultures were resuspended in MMA buffer [10 m m 2-N-Morpholino ethanesulfonic acid (MES), 10 m

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Todd J. Rounsaville, Darren H. Touchell, Thomas G. Ranney, and Frank A. Blazich

·L −1 2-(N-Morpholino) ethanesulfonic acid (MES) monohydrate at 0.1 g·L −1 , solidified with agar at 7.5 g·L −1 , and pH adjusted to 5.75 ± 0.03. Filter-sterilized GA 3 was added to cooled autoclaved media. Excised buds were placed on media and

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Yosef Al Shoffe, Abdul Sattar Shah, Jacqueline F. Nock, and Christopher B. Watkins

m 2-(N-morpholino)ethanesulfonic acid (MES) pH 6.0, 5 m m dithiothreitol, 1 m m phenylmethylsulfonylfluoride, 1% (w/v) polyvinylpyrrolidone, and 0.1% (v/v) Triton X-100. The mixture was homogenized using a vortex and filtered with two layers of

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Shanshan Seng, Jian Wu, Jiahui Liang, Fengqin Zhang, Qiuyan Yang, Junna He, and Mingfang Yi

and 100 μg·mL −1 rifampicin. After overnight culture, the agrobacterium lines were collected and resuspended in infiltration buffer [10 m m MgCl 2 , 200 m m acetosyringone, and 10 m m 2-(N-morpholino) ethanesulfonic acid (pH 5.6)] to a final OD 600

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Yong In Kuk and Ji San Shin

the amount of cellular leakage. This was accomplished by cutting out 4-mm-diameter leaf disks (0.2 g fresh weight) with a cork borer and placing the disks in a 6-cm-diameter polystyrene Petri dish containing 5 mL 1% sucrose and 1 m m 2-( N -morpholino)ethanesulfonic

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Charalambos I. Siminis and Manolis N. Stavrakakis

consisting of 0.7 M sucrose, 10 m m 2-(N-Morpholino)ethanesulfonic acid (MES)-NaOH pH 5.5, 1 m m CaCl 2 , and the cell wall digesting enzymes cellulase R-10 (1%, w/v) and macerozyme R-10 (0.5%, w/v) (Duchefa Biochemie, Haarlem, The Netherlands). Incubation