Proper water management is a major responsibility of managers of creeping bentgrass grown on putting greens in the hot and humid southern states. The combination of shallow root systems, sand-based root zones, high temperatures, and high evaporative demands frequently results in severe drought stress on bentgrass (Agrostis palustris Huds.) greens. This study was initiated to determine the effects of irrigation frequency on creeping bentgrass turgor pressure and on the O2 and CO2 concentrations in a sand-based root zone mixture. In total, 81 plots, 1.5 × 1.5 m each, were established on a USGA-type root zone mixture and organized into 9 groups of 9 plots each. Each group could be irrigated individually. One plot in each group was planted to either `A-4', `Crenshaw', `Mariner', `L-93', or `Penncross' creeping bentgrass. Irrigation frequency treatments of 1-, 2-, and 4-day replacement of historical PET were imposed on three groups each. After establishment, measurements of the leaf water potential, osmotic potential, soil oxygen concentration, and soil carbon dioxide concentrations were made over a 1- to 2-year period. Bentgrass irrigated every 1 or 2 days had significantly (P = 0.05) greater turgor pressures at 0600 hr as compared to turf irrigated every 4 days in 1997. No differences were seen in 1998 due to drier environmental conditions. Concentrations of O2 and CO2 in the soil air remained in the optimal range for all treatments, indicating that lack of O2 in the root zone as a result of frequent irrigation may not be the primary cause for reduced rooting depth of bentgrass grown on highly permeable sand-based root zone mixtures.
John E. Jordan, Richard H. White, James C. Thomas, Trent C. Hale, and Donald M. Vietor
Marc W. van Iersel and Sue Dove
Efficient water use in nurseries is increasingly important. In recent years, new soil moisture sensors (ECH2O probes) have become available, making it possible to monitor the moisture content of the growing medium in containers. One piece of information that is lacking for fully-automated irrigation systems is how much water actually needs to be present in the growing medium to prevent detrimental effects of drought on plants. We determined the effect of substrate moisture on photosynthesis and plant water relations of hydrangea and abelia. Growth rates of these species were measured during two subsequent drying cycles to determine how drought affects the growth rate of these species. Whole-plant photosynthesis, an indicator of growth rate, of both species remained stable as the volumetric moisture content of the substrate dropped from 25% to 15%, with pronounced decreases in photosynthesis at lower substrate moisture levels. Abelias and hydrangeas wilted when the substrate moisture level dropped to 6.3% and 8.3%, respectively. At wilting, abelias had lower leaf water potential (–3.7 MPa) than hydrangeas (–1.8 MPa). After the plants were watered at the end of the first drying cycle, the photosynthesis of the plants did not recover to pre-stress rates, indicating that the drought stress caused a long-term reduction in photosynthesis. Despite the more severe drought stress in the abelias (both a lower substrate water content and lower water potential at wilting), abelias recovered better from drought than hydrangeas. After the plants were watered at the end of the first drying cycle, the photosynthetic rate of abelias recovered to ≈70%, while the photosynthetic rate of the hydrangeas recovered to only 62% of the pre-stress rate.
Krishna S. Nemali and Marc W. van Iersel
Optimal substrate volumetric water content (θ) and drought tolerance of impatiens, petunia, salvia, and vinca were investigated by growing plants under four constant levels of θ (0.09, 0.15, 0.22, and 0.32 m3·m-3). Gas exchange, quantum efficiency (ΦPSII), electron transport rate (ETR), non-photochemical quenching (NPQ), and leaf water potential (ϒ) were measured for all species, and response of photosynthesis (Pn) to internal CO2 concentration (Ci) was studied in petunia and salvia. Leaf photosynthesis (Pmax) was highest at a θ of 0.22 m3·m-3 for all species and did not differ between a θ of 0.15 and 0.22 m3·m-3 for vinca and petunia. The Pn-Ci response curves for petunia were almost identical at a θ of 0.22 and 0.15 m3·m-3. Regardless of species, ETR and ΦPSII were highest and NPQ was lowest at a θ of 0.22 m3·m-3. Based on these results, a θ of 0.22 m3·m-3 for salvia and impatiens and a slightly lower θ of 0.15 m3·m-3 for vinca and petunia, is optimal. Mean osmotic potential in all treatments was lower in vinca and salvia and resulted in higher turgor potential in these species than other species. Analysis of Pn-Ci response curves indicated that Pn at a θ of 0.09 m3·m-3 was limited by both gas phase (stomatal and boundary layer) and non-gas phase (mesophyll) resistance to CO2 transfer in salvia. At the lowest θ level, Pn in petunia was only limited by gas phase resistance, indicating that absence of mesophyll resistance during drought may play a role in the drought tolerance of petunia.
Jason J. Griffin
Common sugar maple (Acer saccharum Marshall) selections suffer from prolonged drought and constant wind on the southern Great Plains. Nonirrigated plants often have scorched and torn leaves as a result of these environmental stresses. In field studies, a sugar maple ecotype native to western Oklahoma (known as `Caddo' maple) has shown improved tolerance to drought and leaf tatter. A study to examine drought tolerance of seedling `Caddo' maple compared to typical seedling sugar maple was established at the John C. Pair Horticultural Center. One seedling of each type was planted in a single 38-L container. Containers were placed on a greenhouse bench, and once acclimated, irrigation was withheld until predawn leaf water potential indicated a substrate water potential of –1.5 MPa. Containers were weighed, and seedlings were maintained in a prolonged drought condition for 3 weeks by adding water each morning to return the container to the original weight. After 3 weeks, photosynthetic temperature response curves were generated for the drought-stressed and the irrigated control plants. Osmotic potential of expressed sap was also measured on rehydrated leaves. The main effects of species, irrigation, and temperature were all significant. `Caddo' maples were able to maintain a higher rate of net photosynthesis than the typical seedlings when drought stressed and as temperature increased. The optimum temperature for photosynthesis did not significantly differ among treatments (36 °C), whereas the maximum rate of photosynthesis was significantly greater for the `Caddo' maples (41 μmol·m-2·s-1) than the typical sugar maples (16 μmol·m-2·s-1).
Emma L. Locke*, Cecil Stushnoff, Joyce C. Pennycooke, and Michelle Jones
Salinity, drought and temperature frequently limit crop productivity. Transgenic Petunia ×hybrida cv. Mitchell with altered endogenous raffinose family oligosaccharides (RFO) due to over-expression (sense) or under-expression (antisense) of the tomato α-galactosidase gene show that antisense increases in RFO are associated with greater tolerance to freezing stress (Pennycooke et al., 2003). Because vegetative propagules of these antisense lines rooted and established more quickly than their sense counterparts, we hypothesized that antisense lines would also respond to salinity and wilting stress. Salinity treatment plants were exposed to 50-200 mm NaCl graduated 25 mm every 3 days and held at 200 mm for 13 days. Dry-down treatments were watered to pot capacity, then not watered until the onset of wilting. This was repeated in cycles for 26 days. Data were collected on plant growth, root/shoot ratios, and leaf water potential. Fresh and dry weights in four of the six antisense lines exceeded the wild type and sense lines. Osmotic potential for salinity and dry-down plants was 160% to 220% higher than control plants. Pearson correlations revealed that higher osmotic potential was partially associated with higher fresh weight (r = 0.7214, P = 0.02) and root/shoot ratios (r = -0.7414, P = 0.02) in salinity stressed plants. In the dry-down drought stressed plants, osmotic potential was not associated with fresh weight (r = 0.3364, ns) nor root/shoot ratio (r = -0.0431, ns). Salinity stress reduced root mass compared to control and dry down plants. Sense plants grew slowly and were highly variable.
Zhaolong Wang, Bingru Huang, Stacy A. Bonos, and William A. Meyer
Drought is a major factor limiting plant growth, which has been associated with the accumulation of absicsic acid (ABA) in various species. The objective of the study was to determine the relationship between ABA accumulation and drought tolerance for kentucky bluegrass (Poa pratensis L.) during short-term drought stress. Eight kentucky bluegrass cultivars (`Midnight', `A82-204', `RSP', `Alpine', `Moonlight', `Brilliant', `Washington', and `Baruzo') were subjected to drought stress in a growth chamber. Water relations, gas exchange rate, and ABA content of leaves were determined at various times during drought stress. Turf quality decreased with drought duration for all eight cultivars. Leaf ABA content increased linearly with drought stress within 11 days of treatment; the rate of the increase was negatively related to the rate of turf quality decline. The rate of ABA accumulation during drought stress was positively correlated with the rates of decrease in turf quality (r 2 = 0.6346), increase in electrolyte leakage (r 2 = 0.7128), and decrease in relative water content (r 2 = 0.5913). There were highly significant negative correlations between ABA content and leaf water potential (r 2 = 0.9074), stomatal conductance (r 2 = 0.6088), transpiration rate (r 2 = 0.6581), net photosynthesis rate (r 2 = 0.6956), and a positive correlation between ABA content and electrolyte leakage (r 2 = 0.7287). The results indicate that drought tolerance is negatively related to ABA accumulation during shortterm drought stress. ABA accumulation in response to drought stress could be used as a metabolic factor to select for drought tolerance in kentucky bluegrass.
Thayne Montague and Lindsey Fox
Recent droughts and depleted water tables across many regions have elevated the necessity to irrigate field-grown (FG) nursery trees. At the same time, ordinances restricting nursery irrigation volume (often without regard to plant water requirements) have been implemented. This research investigated gas exchange and growth of two FG maple tree species (Acer × freemanii `Autumn Blaze' and A. truncatum) subjected to three reference evapotranspiration (ETo) irrigation regimes (100%, 60%, and 30% of ETo) in a semi-arid climate. During Spring 2002, nine containerized (11.3 L) trees of each species were field planted in a randomized block design. Each year trees were irrigated through a drip irrigation system. During the first growing season, all trees were irrigated at 100% ETo. Irrigation treatments began Spring of 2003. Gas exchange data (pre-dawn leaf water potential and midday stomatal conductance) were collected during the 2003 and 2004 growing seasons and growth data (shoot elongation, caliper increase, and leaf area) were collected at the end of each growing season. For each species, yearly data indicates irrigation regime influenced gas exchange and growth of these FG trees. However, it is interesting to note gas exchange and growth of these FG maple trees were not necessarily associated with trees receiving the high irrigation treatment. In addition, it appears the influence of irrigation volume on the growth of these FG trees is plant structure and species specific. Our data suggests irrigation of FG trees based upon local ETo measurements and soil surface root area may be a means to conserve irrigation water and produce FG trees with adequate growth. However, continued research on the influence of reduced irrigation on FG tree species is needed.
Stefania De Pascale, Albino Maggio, Celestino Ruggiero, and Giancarlo Barbieri
We irrigated field-grown celery (Apium graveolens L. var. dulce [Mill.] Pers. 'Tall Utah') with four concentrations of saline water, NSC (nonstressed control), SW1, SW2, and SW3, corresponding to EC of 0.5, 4.4, 8.5, and 15.7 dS·m-1, respectively, plus a nonirrigated control (NIC) and investigated the effects of the treatments on water relations, yield and ion content. In addition, we compared simultaneously plant response to both salt and drought stress by using a modified version of the threshold-slope model. Increasing salinity of the irrigation water reduced fresh and dry weights of the shoots, but increased the dry matter percentage in shoots. The marketable yield was moderately affected by salinity (25% reduction at EC 8.5 dS·m-1). In contrast, a severe water stress dramatically decreased the marketable yield from 23 t·ha-1 (average of the irrigated treatments) to <7 t·ha-1 (nonirrigated control). Na+ and Cl- concentrations increased in salinized plants whereas nitrogen content, K+, Ca2+, and Mg2+ concentrations decreased upon salinization. Midday leaf water potentials (Ψt) decreased from -1.48 MPa (0.5 dS·m-1) to -2.05 MPa (15.7 dS·m-1) and - 2.17 MPa (nonirrigated control), though the reduction in leaf cellular turgor was less severe. The maintenance of high leaf cellular turgor was positively correlated to a decrease in osmotic potential and to an increased bulk modulus of elasticity. These results indicate that it is possible to irrigate celery with saline water (up to 8.5 dS·m-1) with acceptable losses in marketable yield and confirmed that in the field, this species has the ability to efficiently regulate water and ion homeostasis. In the absence of irrigation, celery plants were unable to cope with the drought stress experienced, although this was comparable, in terms of soil water potential, to the one caused by saline irrigation.
Zhaolong Wang, Bingru Huang, and Qingzhang Xu
Abscisic acid (ABA) is an important hormone regulating plant response to drought stress. The objective of this study was to investigate effects of exogenous ABA application on turf performance and physiological activities of kentucky bluegrass (Poa pratensis L.) in response to drought stress. Plants of two kentucky bluegrass cultivars, `Brilliant' (drought susceptible) and `Midnight' (drought tolerant), were treated with ABA (100 μm) or water by foliar application and then grown under drought stress (no irrigation) or well-watered (irrigation on alternate days) conditions in a growth chamber. The two cultivars responded similarly to ABA application under both watering regimes. Foliar application of ABA had no effects on turf quality or physiological parameters under well-watered conditions. ABA application, however, helped maintain higher turf quality and delayed the quality decline during drought stress, compared to the untreated control. ABA-treated plants exposed to drought stress had higher cell membrane stability, as indicated by less electrolyte leakage of leaves, and higher photochemical efficiency, expressed as Fv/Fm, compared to untreated plants. Leaf water potential was not significantly affected, whereas leaf turgor pressure increased with ABA application after 9 and 12 d of drought. Osmotic adjustment increased with ABA application, and was sustained for a longer period of drought in `Midnight' than in `Brilliant'. The results suggested that exogenous ABA application improved turf performance during drought in both drought-sensitive and tolerant cultivars of kentucky bluegrass. This positive effect of ABA could be related to increased osmotic adjustment, cell turgor maintenance, and reduced damage to cell membranes and the photosynthetic system.
Zhaolong Wang and Bingru Huang
Drought is a major limiting factor for turfgrass growth. Understanding genetic variations in physiological responses of turfgrass to drought stress would facilitate breeding and management programs to improve drought resistance. This study was designed to evaluate responses of abscisic acid (ABA) accumulation, water relations, and gas exchange to drought stress in four Kentucky bluegrass (Poa pratensis L.) cultivars differing in drought resistance. Plants of `Midnight' and `A82-204' (drought resistant) and `Brilliant' and `RSP' (drought susceptible) were grown under well-watered (control) or drought stress conditions for 25 days in growth chambers. Turf quality, leaf water potential (Ψleaf), relative water content (RWC), leaf net photosynthesis rate (Pn), and stomatal conductance (gs) declined, while electrolyte leakage (EL) increased during drought progression in all cultivars. The magnitudes of the change in these parameters were greater for `RSP' and `Brilliant' than for `Midnight' and `A82-204'. Leaf ABA content in `RSP' and `Brilliant' increased sharply after 2 days of stress to as much as 34 times the control level at 10 days of drought. Leaf ABA content in `Midnight' and `A82-204' also increased with drought, but to a lesser extent than in the other two cultivars. Leaf ABA level was negatively correlated with Ψleaf and gs. `A82-204' had a significantly lower ABA accumulation rate with changes in Ψleaf during drought compared to `Midnight', `RSP' and `Brilliant'; however, no differences in ABA accumulation rate were detected among the latter three cultivars. In addition, leaf gs was more sensitive to changes in ABA accumulation in `Midnight' and `A82-204' than in `RSP' and `Brilliant'. These results demonstrated that drought tolerant cultivars of Kentucky bluegrass were characterized by lower ABA accumulation and less severe decline in Ψleaf, Pn, gs, and turf quality during drought stress than drought sensitive cultivars. Drought tolerance of Kentucky bluegrass could be related to sensitivity of stomata to endogenous accumulation of ABA under drought stress conditions.