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Flávia D. Pereira, José Eduardo B.P. Pinto, Luciana D.S. Rosado, Helen C.A. Rodrigues, Suzan K.V. Bertolucci, and Osmar A. Lameira

and cost involved in the micropropagation of A. erectifolius can be greatly minimized. Rooting and acclimatization (hardening). Plantlets often die during the transfer from in vitro to ex vitro conditions ( Miceli et al., 2008 ). In the

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Danny L. Barney, Omar A. Lopez, and Elizabeth King

accurate quantitative measurement. Separate studies are underway to evaluate the effects of root-promoting growth regulators on in vitro and ex vitro rooting of these species. Experimental design and data analysis. The experiment was set up as a randomized

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Hua Q. Zhao, Qing H. He, Li L. Song, Mei F. Hou, and Zhi G. Zhang

petioles of coral bells ( Herchera Sanguinea Engelm.) cultured in vitro, and subsequent planting and flowering ex vitro In Vitro Cell. Dev. Biol. Plant 39 135 138 Ivanova, M. Novák, O. Strnad, M. Van Staden, J. 2006 Endogenous cytokinins in shoots of Aloe

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Christopher J. Biai, José G. Garzon, Jason A. Osborne, Jonathan R. Schultheis, Ronald J. Gehl, and Christopher C. Gunter

. Wilhelmova, N. Synkova, H. Catsky, J. Krebs, D. Ticha, I. Hanakova, B. Snopek, J. 1998 Acclimation of tobacco plantlets to ex vitro conditions as affected by application of abscisic acid J. Expt. Bot. 49 863 869 Seo, M. Koshiba, T. 2002 Complex regulation of

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Jin Cui, Juanxu Liu, Jianjun Chen, and Richard J. Henny

Institute, Cary, NC), and mean separations were determined using Fisher's protected least significant differences at the 5% levels. Rooting and ex vitro plantlet establishment. Shoots (≈3 to 4 cm) were excised and transferred to Magenta GA-7 vessels

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I-Chun Pan, Ya-Fen Lu, Pei-Jung Wen, and Yen-Ming Chen

axillary buds with colchicine ex vitro. The results demonstrated that different application methods (cotton vs. lanoline) and colchicine concentrations affected the induction efficiency of polyploidy and bud survival rate. In ‘Dulce Rosa’, no correlation

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Maurizio Micheli, Daniel Fernandes da Silva, Daniela Farinelli, Graziana Agate, Rafael Pio, and Franco Famiani

been reported that longer uninodal explants can be subcultured better ( Sghir et al., 2005 ). Moreover, obtaining more robust explants is also important at the time of transplanting to ex vitro conditions, because vigorous plants are more capable of

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Fan Li, Guoxian Wang, Rongpei Yu, Min Wu, Qinli Shan, Lifang Wu, Jiwei Ruan, and Chunmei Yang

Gypsophila. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology 149:322–328 Wang, S.M. Piao, X.C. Park, S.Y. Lian, M.L. 2013 Improved micropropagation of Gypsophila paniculata with bioreactor and factors affecting ex vitro

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Lucia E. Villavicencio, James A. Bethke, and Lea Corkidi

Sumagic (uniconazole) promotes flower bud set on Camellia japonica J. Environ. Hort. 23 153 157 Cha-um, S. Puthea, O. Kirdamanee, C. 2009 An effective in-vitro acclimatization using uniconazole treatments and ex-vitro adaptation of Phalaenopsis orchid

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Seong Min Woo and Hazel Y. Wetzstein

secondary elongation medium supplemented with (2-isopentenyl) adenine. Shoots readily root ex vitro with high survival rates. Outplanting studies of in vitro-generated plants are underway in native sites. Thus, tissue culture appears to be a promising