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Chunqing Sun, Zhihu Ma, Zhenchao Zhang, Guosheng Sun, and Zhongliang Dai

valuable. Determination of pollen viability. Pollen grains from newly split anthers were collected from 0900 to 1000 hr on sunny days. The samples were placed on a glass slide coated with culture medium including 100 g·L −1 polyethylene glycol (PEG4000

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Chunxian Chen, Jude W. Grosser, Milica Ćalović, Patricia Serrano, Gemma Pasquali, Julie Gmitter, and Fred G. Gmitter Jr

( Grosser and Gmitter, 1990 ; Grosser et al., 2000 ). Fusions were conducted in 60 × 15-mm polystyrene petri dishes using our standard PEG (40% polyethylene glycol) volumetric plating method ( Grosser and Gmitter, 1990 ). After fusion, protoplasts were

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Mohamad-Hossein Sheikh-Mohamadi, Nematollah Etemadi, and Mostafa Arab

Membrane permeability and micro- and macro-element accumulation in spring wheat cultivars during the short-term effect of salinity- and PEG-induced water stress Acta Physiol. Plant. 34 985 995 Fry, J.D. Lang, S.N. Clifton, R. Maier, F.P. 1993 Freezing

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David H Suchoff, Christopher C. Gunter, Jonathan R. Schultheis, Matthew D. Kleinhenz, and Frank J. Louws

increase in relative water content we observed when grafting onto ‘Beaufort’ is in agreement with the findings of Altunlu and Gul (2012) . These authors found that under PEG-induced drought stress, ‘Beaufort’ improved relative water content in the scion

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Huiling Wang, Wei Wang, Weidong Huang, and Haiying Xu

248 267 279 Wang, H.L. Wang, W. Zhan, J.C. Huang, W.D. Xu, H.Y. 2015 An efficient PEG-mediated transient gene expression system in grape protoplasts and its application in subcellular localization studies of flavonoids biosynthesis enzymes Scientia

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Zhou Li, Yan Peng, and Bingru Huang

of drought-tolerant transgenic rice plants Front. Plant Sci. 6 84 Wang, C.Q. 2010 Exogenous calcium alters activities of antioxidant enzymes in Trifolium repens L. leaves under peg-induced water deficit J. Plant Nutr. 33 1874 1885 Wang, W. Vinocur

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Zhou Li, Yan Peng, and Bingru Huang

-Aminobutyric acid (GABA) priming enhances the osmotic stress tolerance in Piper nigrum Linn. plants subjected to PEG-induced stress Plant Growth Regulat., doi: 10.1007/s10725-015-0074-6. Wang, K.H. Zhang, X.Z. Ervin, E. 2012 Antioxidative responses in roots and

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Richard V. Tyson, Danielle D. Treadwell, and Eric H. Simonne

://water.epa.gov/aboutow/upload/71issue.pdf >. Verhagen, F.J.M. Hageman, P.E.G. Woldendorp, J.W. Laanbroek, H.J. 1994 Competition for ammonium between nitrifying bacteria and plant roots in soil pots: Effects of grazing flagellates and fertilization Soil Biol. Biochem. 26 89 96

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Yuliya A. Salanenka, Martin C. Goffinet, and Alan G. Taylor

outer anticlinal cell wall (as “pegs”) of adjacent epidermal cells, a typical construction of plant cuticles. Considering that the lipid layer of the PE envelope is derived from the epidermis of the nucellus ( Ramakrishna and Amritphale, 2005 ), the

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Suejin Park, Youyoun Moon, and Nicole L. Waterland

of the effects of mannitol and PEG osmotic stress on growth and solute accumulation in Sesuvium portulacastrum Environ. Exp. Bot. 61 10 17 Suwa, R. Jayachandran, K. Nguyen, N.T. Boulenouar, A. Fujita, K. Saneoka, H. 2008 Barium toxicity effects in