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results were observed in hydroprimed and KNO 3 -primed sunflower ( Helianthus annuus L.) under saline and drought conditions ( Kaya et al., 2006 ). Polyethelene glycol 8000 (PEG8000)-primed soybean ( Glycine max L.) showed enhanced seed germination and

) with 10 mL of a polyethylene glycol [PEG (PEG 8000, Sigma-Aldrich, St. Louis, MO)] solution at ψ S of −1.25 MPa and then sealed with laboratory film (parafilm M; Bemis Flexible Packaging, Neenah, WI). The PEG concentration was calculated according to

modified PEG method ( Rowland and Nguyen, 1993 ) with Plant DNAzol Reagent (Invitrogen, Carlsbad, CA) as described by Sugimoto et al. (2005) . Polymerase chain reaction (PCR) was performed by the GeneAmp PCR System Model 9700 (Applied Biosystems, Foster

gene banks for strawberries ( Table 1 ). Total DNA was extracted by using a modified PEG method ( Rowland and Nguyen, 1993 ) with Plant DNAzol Reagent (Invitrogen, Carlsbad, CA), as described by Sugimoto et al. (2005) . Two noncoding regions of cpDNA

mL distilled water or 10 mL of solution containing various concentrations of (±) abscisic acid (ABA; 12.5, 25, 50, and 100 μ m ; Sigma-Aldrich, St. Louis, MO) or polyethylene glycol (PEG 8000; Sigma-Aldrich) and placed in petri dishes (9 cm diameter

10 mL of distilled water, a solution of (±) abscisic acid (ABA; Sigma-Aldrich, St. Louis, MO) or polyethylene glycol (PEG 8000, Sigma-Aldrich) and placed in 9-cm petri dishes. The PEG concentrations were calculated to obtain water potentials of −0

( Hoaglandand Arnon, 1937 ), under the fluorescent lighting (200 μE·m −2 ·s −1 ) at 22 to 24 °C and a 14/10-h light/dark cycle 60% relative humidity and then irrigated with 200 mmol·L −1 NaCl and 15% polyethylene glycol (PEG), respectively, for 24 h. Each

stain (3% w/v polyethylene glycol (PEG) 6000, 5 µg·mL −1 of PI, 180 units/mL RNase, 0.1% Triton X-100 in 4 m m citrate buffer). The solution was transferred to a 1.5-mL tube and incubated in a water bath for 20 min at 37 °C. PI salt stain (3% w/v PEG

of sweetpotato [ Ipomoea batatas (L.) Lam.] under PEG-induced water stress Not. Bot. Horti Agrobot. Cluj-Napoca 45 164 Zhang, C. Shi, S. 2018 Physiological and proteomic responses of contrasting alfalfa ( Medicago sativa L.) varieties to PEG

investigations are necessary to determine if mixing an osmotic or matric inhibitor such as polyethylene glycol (PEG) into the R. asiaticus hydration solution will alleviate symptoms observed at potentially damaging temperatures. Because the PEG solution would