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Qi Zhang, Kevin Rue, and Jeanna Mueller

results were observed in hydroprimed and KNO 3 -primed sunflower ( Helianthus annuus L.) under saline and drought conditions ( Kaya et al., 2006 ). Polyethelene glycol 8000 (PEG8000)-primed soybean ( Glycine max L.) showed enhanced seed germination and

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Isabel Pimentel and Samuel Contreras

) with 10 mL of a polyethylene glycol [PEG (PEG 8000, Sigma-Aldrich, St. Louis, MO)] solution at ψ S of −1.25 MPa and then sealed with laboratory film (parafilm M; Bemis Flexible Packaging, Neenah, WI). The PEG concentration was calculated according to

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Masanori Honjo, Susumu Yui, and Miyuki Kunihisa

modified PEG method ( Rowland and Nguyen, 1993 ) with Plant DNAzol Reagent (Invitrogen, Carlsbad, CA) as described by Sugimoto et al. (2005) . Polymerase chain reaction (PCR) was performed by the GeneAmp PCR System Model 9700 (Applied Biosystems, Foster

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Masanori Honjo, Sono Kataoka, Susumu Yui, Masami Morishita, Miyuki Kunihisa, Takayoshi Yano, Megumi Hamano, and Hiromichi Yamazaki

gene banks for strawberries ( Table 1 ). Total DNA was extracted by using a modified PEG method ( Rowland and Nguyen, 1993 ) with Plant DNAzol Reagent (Invitrogen, Carlsbad, CA), as described by Sugimoto et al. (2005) . Two noncoding regions of cpDNA

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Samuel Contreras, Mark A. Bennett, and David Tay

mL distilled water or 10 mL of solution containing various concentrations of (±) abscisic acid (ABA; 12.5, 25, 50, and 100 μ m ; Sigma-Aldrich, St. Louis, MO) or polyethylene glycol (PEG 8000; Sigma-Aldrich) and placed in petri dishes (9 cm diameter

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Samuel Contreras, Mark A. Bennett, James D. Metzger, and David Tay

10 mL of distilled water, a solution of (±) abscisic acid (ABA; Sigma-Aldrich, St. Louis, MO) or polyethylene glycol (PEG 8000, Sigma-Aldrich) and placed in 9-cm petri dishes. The PEG concentrations were calculated to obtain water potentials of −0

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Yingmei Gao, Jingkang Hu, Tingting Zhao, Xiangyang Xu, Jingbin Jiang, and Jingfu Li

( Hoaglandand Arnon, 1937 ), under the fluorescent lighting (200 μE·m −2 ·s −1 ) at 22 to 24 °C and a 14/10-h light/dark cycle 60% relative humidity and then irrigated with 200 mmol·L −1 NaCl and 15% polyethylene glycol (PEG), respectively, for 24 h. Each

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Sumin Kim, Mengqiao Han, and A. Lane Rayburn

stain (3% w/v polyethylene glycol (PEG) 6000, 5 µg·mL −1 of PI, 180 units/mL RNase, 0.1% Triton X-100 in 4 m m citrate buffer). The solution was transferred to a 1.5-mL tube and incubated in a water bath for 20 min at 37 °C. PI salt stain (3% w/v PEG

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Qi Wang, Rui Zhao, Qihang Chen, Jaime A. Teixeira da Silva, Liqi Chen, and Xiaonan Yu

of sweetpotato [ Ipomoea batatas (L.) Lam.] under PEG-induced water stress Not. Bot. Horti Agrobot. Cluj-Napoca 45 164 Zhang, C. Shi, S. 2018 Physiological and proteomic responses of contrasting alfalfa ( Medicago sativa L.) varieties to PEG

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Christopher B. Cerveny, William B. Miller, Thomas Björkman, and Neil S. Mattson

investigations are necessary to determine if mixing an osmotic or matric inhibitor such as polyethylene glycol (PEG) into the R. asiaticus hydration solution will alleviate symptoms observed at potentially damaging temperatures. Because the PEG solution would