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Ryan N. Contreras, John M. Ruter, and Wayne W. Hanna

induce polyploidy in seedlings from H. acetosella ‘Panama Red’ and to evaluate the effects on growth, morphology, and fecundity. Upon discovering that treatments induced two cytochimeras, ploidy of the histogenic layers of these plants was investigated

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Hisayo Yamane, Megumi Ichiki, Ryutaro Tao, Tomoya Esumi, Keizo Yonemori, Takeshi Niikawa, and Hino Motosugi

) suggested the genetic identity between TTN and HTN. In this report, we determined the ploidy level of the two cultivars. Then, to investigate the growth characteristics of TTN, we compared the morphological characteristics of vegetative and reproductive

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Chunlan Li, Panyun Xu, Aote Zhou, Jinlong Song, Yuxia Wu, and Tianming He

without pests and disease. Detection of ploidy in ‘Mianli’. Stem tip chromosome preparation was performed from the end of March to mid-April 2020, from 9:00 am to 11:00 am . Actively growing stem tips (0.5 cm at the center of stem tip) were cut

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Jude W. Grosser, Hyun Joo An, Milica Calovic, Dong H. Lee, Chunxian Chen, Monica Vasconcellos, and Frederick G. Gmitter Jr

room. Plant regeneration and ploidy analysis. Regenerating embryogenic calli and embyoids were transferred to solid EME medium containing 50 g·L −1 maltose ( Perez et al., 1998 ) for somatic embryo induction according to Grosser and Gmitter

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Milica Ćalović, Chunxian Chen, Qibin Yu, Vladimir Orbović, Frederick G. Gmitter Jr, and Jude W. Grosser

/maintenance, suspension maintenance, and plant regeneration from protoplasts were adjusted to pH 5.8 with 1 N NaOH and autoclaved at 121 °C for 20 min. All media and solutions for protoplast isolation, fusion, and culture were filter-sterilized (0.22 μm pore size). Ploidy

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Lauren E. Deans, Irene E. Palmer, Darren H. Touchell, and Thomas G. Ranney

developed tetraploid of H. macrophylla ‘David Ramsey’ was also included to compare morphological characters between ploidy levels. ‘Robert’ and ‘David Ramsey’ have mophead blooms; ‘MAK20’ is a lacecap. All cultivars are purportedly remontant. In

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Wenjing Pang, John E. Luc, William T. Crow, Kevin E. Kenworthy, Robert McSorley, and Robin M. Giblin-Davis

and decreased rooting under sting nematode pressure when compared with ‘Tifway’. No obvious relationship was found to exist between rooting response and ploidy level or origin of the accessions. This information should be of value to select parents for

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Whitney D. Phillips, Thomas G. Ranney, Darren H. Touchell, and Thomas A. Eaker

]} ( Table 1 ). Six additional interspecific hybrids were also interspersed with the triploids. Plants were completely randomized. Table 1. Ploidy, relative holoploid genome size, female reproductive characteristics, and reproductive pathways for selected

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Josh A. Honig, Stacy A. Bonos, and William A. Meyer

; Muntzing, 1933 ; Nielsen, 1946 ; Tinney, 1940 ). Although this complex polyploidy may sometimes present a challenge to breeding efforts in this species, the ability of kentucky bluegrass to propagate diverse and odd ploidy levels through apomixis results

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Mark K. Ehlenfeldt and Matthew Kramer

cultivars were pollinated under greenhouse conditions with either self-pollen or a multicultivar bulk-pollen mixture (appropriate to ploidy level and species) to determine their relative responses to self-pollination and cross-pollination. The hexaploid