Brassica napus (genome aacc), a natural allotetraploid derived from hybridization between B. oleracea L. (genome cc) and B. rapa L. (genome aa), was resynthesized by somatic and sexual hybridization. Seventy-two interspecific somatic (R0) hybrids and 27 sexual (F1) hybrids were produced from the same parent plants. R0 and F1 hybrids displayed morphology that was intermediate to the species parents, but B. rapa characteristics tended to predominate. R0 hybrids with nuclear DNA content equivalent to natural B. napus were uniform for nuclear-encoded traits, whereas allotetraploid F1 hybrids were variable for traits such as morphology, flower color, and seed production. Chloroplast restriction fragment length polymorphisms (RFLPs) showed unequal segregation in the R0 population favoring the chloroplasts of B. rapa; two of the 58 R0 hybrids tested had only the B. oleracea marker and 10 contained markers of both parents. Mitochondrial RFLPs showed a similar bias among the 56 R0 hybrids tested; only four plants showed B. oleracea markers exclusively, and the remaining plants were evenly distributed between having only B. rapa markers or having combinations from both species. In contrast, sexual hybrids displayed only maternal organelle markers.
Richard H. Ozminkowski Jr. and Pablo Jourdan
David M. Czarnecki II, Zhanao Deng, Madguhuri N. Rao, Frederick G. Gmitter Jr., Young A. Choi, Jeffrey G. Norcini, and David G. Clark
As one of the Florida's state wildflowers, Coreopsis leavenworthii is highly desirable for roadside plantings in all parts of the state. Seeds of this species are being produced by growers. Where should seed be produced for different ecotypes? Where can the seed be used? These are among questions that have arisen in commercial seed production and distribution. To address these questions, it was necessary to assess the levels of genetic diversity. Eleven populations (242 total individuals) were collected from different parts of Florida, grown at one location in central Florida, and observed for morphological variations. North Florida natural populations had more complex leaves, while south Florida natural populations had smaller flowers. Principal component analyses revealed that two of the seven characteristics studied accounted for as much as 88% of the morphological variations observed. Molecular diversity was analyzed by using the fluorescent amplified fragment length polymorphism (AFLP) technique and the capillary sequencing system. Four primer combinations detected 320 AFLP fragments, of which 90.6% were polymorphic. The overall genetic diversity in the species was 0.2206 (estimated using AMOVA), of which 77.9% was within populations and 22.1% was among populations. The genetic distance among populations seemed to be loosely correlated with geographical distances. A high level of gene flow was found in several populations. Based on the results, a model has been developed to describe the genetic relationship of Coreopsis leavenworthii populations.
In plant germplasm conservation, “orthodox” seed (i.e. seed that survives drying down to low moisture content) is the most suitable propagule for long-term storage. In general, high quality seeds of around 5% seed moisture content can be stored for 5-15 years at 2°C and 15-50 years at -18°C. Globally, there are some 1,300 genebanks and 6.1 million accessions of food and industrial crops in conservation. When collecting and conserving plant germplasm, seed science and technology have to be applied during germplasm collection; seed regeneration-germination, seedling establishment, flower synchronization, pollination, harvesting, drying, processing and packaging; seed storage and conservation; characterization and evaluation; and finally, distribution. Some of the seed science knowledge and technology skills encompass seed sampling strategy, sample size, seed health, germination and vigor testing, dormancy breaking, scarification, stratification, vernalization, photoperiod treatment, isolation and pollination techniques, harvesting, threshing, drying, hermetic packaging, storage facility design, etc. The goal is to produce seed lots that fulfill the required genetic, physical, physiological and health quality. A summary was presented to relate germplasm conservation activities to seed science and technology. Some of the seed production, processing and testing equipment used were highlighted. Seed research in germplasm conservation is therefore crucial to streamline the operation and management of a genebank to make it more cost effective and attractive for funding.
Rita L. Hummel and Wilbur C. Anderson
Cabbage seed production in western Washington is at risk from freeze damage in the months of November to February. During the 1987-1988, 1988-1989 and 1989-1990 winters, the cold protection efficacy of 5 floating row covers (Agryl P17, Dewitt N-sulate, Reemay 2014, DuPont Typar, VisQueen Porous Row Cover) and straw was tested on field-grown cabbage. Air temperature in the cabbage crown, Tk50 of cabbage leaves, plant winter survival and seed yield were measured. During a severe freeze in February 1989, an average temperature of -11.1 °C was recorded in the uncovered controls while temperatures under the row covers were -6.7°C, -6.8°C and -8.4 °C under the N-sulate, VisQueen and Agryl covers, respectively. When compared to controls in June of 1989, row covers increased the survival of the more cold hardy `Brunswick' plants but did not significantly increase seed yields. The duration and severity of the February 1989 freeze was such that all of the less cold hardy `Golden Acre' plants were killed.
Ingrith D. Martinez and P.M. Lyrene
Fruit set, fruit size, and seed production after hand pollination in a greenhouse were compared for southern highbush blueberry managed in two ways: a) 69 clones were allowed to go dormant and lose their leaves in the field before being dug and subjected to 1000 hours at 5 °C and b) 26 clones were kept growing in a greenhouse through fall and winter without leaf loss and without chilling, to induce flowering on plants that had mature leaves. On each plant in both management systems, some flowers were self-pollinated, some were cross-pollinated, and others had the styles removed before anthesis to prevent pollination. For >1000 flowers per pollination treatment on the deciduous plants, fruit set averaged 1% for no pollination, 46% for self-pollination, and 76% for cross-pollination. The corresponding values for the evergreen plants were 23%, 59%, and 81%. Parthenocarpic berries averaged 0.37 g/berry for deciduous plants and 1.01 g for evergreen plants. Both crossed and selfed berry weights averaged slightly higher for the evergreen plants than for the deciduous plants, but seed number per berry was much lower for the evergreen plants (12 seeds in crossed berries and four seeds in selfed berries) compared to deciduous plants (37 and 8). Southern highbush blueberry plants that flower without going dormant appear to have much higher parthenocarpic capabilities than those that flower after a dormant period.
Civil war and the hostilities which followed it in Cambodia from 1972 to 1979 resulted in a 20% reduction in the country's population and the near total destruction of its educational and agricultural research infrastructure. As if this were not enough, western governments embargoed humanitarian aid to Cambodia during its most critical period of need from 1981 until multiparty elections were held in 1993. During this period a handful of nongovernmental agencies helped the government begin rebuilding some of its agricultural production capacity. One NGO, together with its government counterparts, established the country's first research station for vegetable crops in 1985 at the request of the Ministry of Agriculture. The Kbal Koh Vegetable Crops Research Station was built and its staff received training from 1985 to 1987. The facility has continued its four-part mission with very limited outside funding and technical support since 1987. Numerous variety and seed production trials have been conducted at the station and in farmers' fields since 1985; practical training programs for agricultural technicians and students began in 1986 and today provide much of the salary and operating budget support for the station. Coinciding with the phase out of NGO assistance in 1995, their are great expectations for continuing support through the newly formed Cambodia–Laos–Vietnam vegetable production and research network, AVRDC, and the Asian Development Bank.
Bassam Al-Safadi and Philipp W. Simon
Carrot tissue cultures, germinating seed, and dry seed were exposed to gamma radiation. Irradiation accelerated germination of carrot seed in the M1 generation at low doses (0.5 and 1 krad), whereas higher doses delayed germination. A high negative correlation was observed between dose and survival of plants after seed irradiation. Plant size and root weight were 20 % to 35% greater than control plants after seeds, but not tissue cultures, were exposed to low doses of gamma irradiation. Higher doses reduced M1 plant size by >50% in germinating seed and tissue culture treatments but less for the dry seed treatment. Seed production decreased while phenotypic variation of M1 plants increased with increasing gamma ray dosage. Root weight and total dissolved solids were highly variable in M2 families. Less variation was observed in total carotene content and none was seen in sugar type (reducing vs. nonreducing sugars). Induced variation in root color and root shape was also observed. Irradiation of germinating seed and tissue cultures yielded more M2 variation than irradiation of dry seed. Putative point mutations were not observed. Unirradiated carrot tissue cultures did not yield significant M2 somaclonal variation. Average root weight of M2 plants increased with increasing gamma ray dosage, especially for the dry seed treatment.
R.A. Malvar, P. Revilla, P. Velasco, M.E. Cartea, and A. Ordás
The pink stem borer (PSB) (Sesamia nonagrioides Lefebvre) and the European corn borer (ECB) (Ostrinia nubilalis Hübner) are the major insect pests of corn (Zea mays L.) in Mediterranean countries, although larvae of other insects can also cause damage. The objective of this research was to evaluate the effect of sweet corn hybrids, planting time, and environment on damage by various insects. Data were recorded on the number of larvae of each of the aforementioned pests and damage produced in the ear 20 days after pollination and in the ear and stem when plants were dry. PSB was the most abundant pest, followed by ECB. Other insects, such as Mythimna unipuncta (Haworth) and Helicoverpa armigera (Hübner) were rarely found in sweet corn plants. ECB was constant over time, PSB had larger seasonal variation, and M. unipuncta and H. armigera were highly variable over time. There were significant differences among planting dates and growing cycles for damage traits in each environment. Interactions among hybrids, planting dates, and environments were significant. Dry ears were damaged more than fresh ears and stems had more larvae than ears. The economic value of the crop was seriously affected because most fresh ears had some damage, and seed production would be severely affected by PSB.
Jonathan R. Schultheis, Wanda W. Collins, and Charles W. Averre
Micropropagated sweetpotato is utilized in California as pan of its seed production program. Sweetpotato yields and quality might be improved in North Carolina by incorporating micropropagation as pan of its plant production scheme. Field studies were conducted in 1992 and 1993 to determine the effects of micropropagation, virus, and hill selection on yield and qualify of Jewel and Beauregard varieties. In 1992, yield was increased in Beauregard with micropropagated plants compared with plants that were derived from the North Carolina seed program. However, no yield advantage was measured when Jewel was micropropagated. In 1993, yield from micropropagated Beauregard sweetpotato plants was the same as when plant material was derived from the North Carolina Certified Seed program. Virus-free micropropagated Beauregard plants yielded more number one and canner grade roots than micropropagated plants that harbored the virus at planting. Russet crack symptoms were significantly greater in roots that were not micropropagated compared with micropropagated plants. Total marketable yield of Jewel was increased when obtained from micropropagated versus nonmicropropagated plant stock. Micropropagated Jewel obtained from a California selection yielded nearly 20% less than the North Carolina selection. Yields from micropropagated planting stocks consistently were equal to or better (typically 10 to 20%) than from plant stock not micropropagated.
Sylvia Letícia Oliveira Silva, Renato de Mello Prado, Gilmara Pereira da Silva, Gabriel Barbosa da Silva Júnior, Monica Lanzoni Rossi, and Leónides Castellanos González
This study aimed to evaluate the effects of boron (B) omission on cowpea nutrition and to compare the impact of foliar B fertilization with and without sorbitol on cowpea growth, nutritional status, and B uptake. Two trials using a completely randomized experimental design were conducted. During the first experiment, nutrient solution was provided without B (−B) and with B (+B) in 10 replicates. During the second experiment, a 5 × 2 factorial treatment scheme was used. Five B concentrations (0, 1.25, 2.5, 3.75, and 5.0 g·L−1) were administered foliarly in the form of boric acid with or without sorbitol (500 mmol·L−1) in four replicates. B omission symptoms, root growth, plant organ dry mass and B content, and grain yield were evaluated. B omission induced greater losses in reproductive organ and root growth than in leaf and stem production. It also caused deformation of the middle lamella and accumulation of starch in the chloroplasts. Foliar applications of 2.6 to 2.9 g·L−1 B improved cowpea production. The addition of sorbitol did not enhance plant growth. However, it increased B absorption in the vegetative parts of the plant but did not enhance seed production.