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) with an automated titrimeter and electrode standardized to pH 2.0, 4.0, 7.0, and 10.0 buffers. Titratable acidity was determined using 6 g of juice diluted with 50 mL of deionized, degassed water by titration of 0.1 N sodium hydroxide to an endpoint of

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-H refill cartridge (30 × 4.5 mm) was used for a guard column. Columns were maintained at 65 °C by a temperature control unit. Mobile phase consisted of a pH 2.28 solution of sulfuric acid and water with a resistivity of 18 M obtained from a Millipore Milli

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. The chromatographic column (3 μm, 150 × 3 mm) was an Acclaim 120 C18 (Dionex, Sunnyvale, CA), and the column temperature was 30 °C. Mobile phase A was acetonitrile, mobile phase B was water (adjusted to pH 2.6 with acetic acid), and the flow rate was 0

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of 5.8 ( Lien and Rognes, 1997 ); a pH increase from 3.5 to 9.2 causes a reduction in glycine uptake in Lolium perenne ( Thornton, 2001 ), and glycine uptake by Pisum sativum shows little change between pH 2 to 9 ( Dureja et al., 1984 ). Glycine

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was carried out on a 250 × 4.6-mm, 5-μm column (Diamonsil C18; Dikma Technologies, Foothill Ranch, CA). Detection was operated at 214-nm wavelength with 0.1 mmol·L −1 KH 2 PO 4 (pH = 2.6) as mobile phase passed through a 0.45-μm membrane filter. The

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(pH 2.0, using 6 M HCl). With a separatory funnel, the free phenolic compounds were extracted with diethyl ether (5 × 20-mL portions). The extractant was composed of acetone, water, and acetic acid. The organic phase (acetone) was removed using the

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(HSD) test at α = 0.05. A change in pH (Δ pH) represents the difference between the starting pH of 5.8 and measured pH 2 d after nutrient solution replacement. Adding MES to the nutrient solution did not affect plant growth, as evaluated by runner

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globe, red, medium-firm, green shoulder, avg. 175 g. Determinate. Resistance: Verticillium wilt race 1, Fusarium wilt races 1 and 2, early blight (stems), late blight (Ph2 and Ph3 heterozygous.) Adaptation: temperate. 2011. Five Star Grape —Breeder and

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; attractive; very good flavor; soluble solids usually 11-12 °Brix; pH 2.8 titratable acidity 6.8 g·L –1 ; ripens mid-early on floricanes, 3 d before Ouachita in Arkansas; very good postharvest potential and recommended for commercial shipping. Plant: erect

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