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Weiping Zhong, Zhoujun Zhu, Fen Ouyang, Qi Qiu, Xiaoming Fan, and Deyi Yuan

reproductive organ of angiosperms. The development of anthers, the parts of the stamen where pollen is produced, is a delicate and complex process that includes the formation of the anther wall tissue (with a protective function) and pollen (with a reproductive

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Alisha L. Ruple, John R. Clark, and M. Elena Garcia

the slide. One to two drops of aniline blue stain were placed on top of the pollen, and samples were covered and allowed to stain at least 10 min ( Kearns and Inouye, 1993 ). Samples were viewed under a light microscope and 500 pollen grains were

Open access

Alberto Sánchez-Estrada and Julián Cuevas

orchard. CP treatments were achieved by applying either ‘Barouni’ fresh pollen or purchased ‘Sevillano’ pollen to the stigmas of ‘Manzanillo’ flowers using a fine paintbrush. Pollen viability was ascertained before use by the fluorochromatic staining test

Open access

Guanxing Hu, Chao Gao, Xiaoming Fan, Wenfang Gong, and Deyi Yuan

Xenia is defined as the effect of foreign pollen on the development of fruit tissue. It involves the interaction between a nuclear gene from the male gamete and either two polar nuclear genes from the endosperm or one nuclear gene from the egg

Open access

Guirong Li, Ran Quan, Chaohui Yan, Xiaojin Hou, and Huiling Hu

deionized water. Fresh solutions were prepared for each experiment. Pollen was stained in a working solution for 30 min before observation. Counts were made at random in three fields, and pollen was photographed under an inverted fluorescence microscope [10

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María Engracia Guerra, Ana Wünsch, Margarita López-Corrales, and Javier Rodrigo

., 1996 ) in polystyrene petri dishes (60 × 10 mm). Pollen was germinated for 24 h at 20 °C and then frozen at –20 °C to arrest pollen germination. Preparations were thawed during 24 h at 4 °C, stained with 1% (v/v) aniline blue in 0.1 n K 3 PO 4 to

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Huan Xiong, Ping Chen, Zhoujun Zhu, Ya Chen, Feng Zou, and Deyi Yuan

heterosis in this species. Male sterility in plants is defined by the loss of function or degeneration of male organs resulting from genetic or physiological factors, and these plants cannot produce normal pollen or anthers ( Yu et al., 2010 ). Male

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Yang Hu, Chao Gao, Quanen Deng, Jie Qiu, Hongli Wei, Lu Yang, Jiajun Xie, and Desheng Liao

cells underwent mitosis to form pollen mother cells that were surrounded by callose. ( B4 ) Callose underwent the first degradation, which was manifested by light staining, and it released single pollen mother cells for meiosis. ( C1 ) The pollen mother

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Bandara Gajanayake, Brian W. Trader, K. Raja Reddy, and Richard L. Harkess

grains were collected as described for the PG procedure from 30 plants in each cultivar between 900 and 1000 hr . Pollen viability was tested using 2.5% concentration of 2, 3, 5-triphenyl tetrazolium chloride (TTC) stain in deionized water as described

Open access

Fengxia Shao, Sen Wang, Zhiming Liu, Rongyan Hong, and Tianjiao Zhao

pollen, embryo sac, embryo, and endosperm developmental process could result in seed abortion ( Liang et al., 2005 ). The embryological mechanism of seed abortion is divided into four aspects: male sterility, female abortion, pollination, and