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Chunqing Sun, Zhihu Ma, Zhenchao Zhang, Guosheng Sun, and Zhongliang Dai

Nymphaea after pollination using aniline blue staining method. ( A ) Pollen grains (Pg) germinating on each stigma at 12 h after pollination in the self-pollinated Nymphaea odorata ‘Peter Slocum’ (scale bar = 50 μm). ( B and C ) Only a few pollen

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Natalia R. Dolce, Ricardo D. Medina, Luis A. Mroginski, and Hebe Y. Rey

staining of orchid seeds for viability testing Amer. Orchid Soc. Bull. 50 416 418 Speranza, A. Calzoni, G.L. Pacini, E. 1997 Occurrence of mono- or disaccharides and polysaccharide reserves in mature pollen grains Sex. Plant Reprod. 10 110 115 van Bilsen, D

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Cecil Pounders, Tim Rinehart, and Hamidou Sakhanokho

100 to determine the percentage of pod set. Pollen staining. Five flowering ‘Tonto’ × L. speciosa seedlings were randomly selected to study pollen viability. Three flowers were collected from the seedlings and from plants of ‘Tonto

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Javier Sanzol and Maria Herrero

brightener used for pollen tube identification in vivo Stain Technol. 49 199 202 Johansen, D.A. 1940 Plant microtechnique McGraw-Hill N.Y Lewis, D. Modlibowska, I. 1942 Genetical studies in

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Cheng-Jung Hu, Nean Lee, and Yung-I Lee

chamber, and the pollinia were taken out for observation at 1, 2, 4, 6, 8, and 10 d of culture. The germinating pollinia were transferred onto slides and gently separated by forceps, then were stained with 0.04% methyl green (Sigma-Aldrich Co.) for pollen

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Chao Gao, Deyi Yuan, Ya Yang, Bifang Wang, Dongming Liu, and Feng Zou

The compatible fertilization of angiosperms is initiated through the hydration of the pollen at the stigma and the germination of the pollen tube. After entering the interpapillar cell space, the tube grows in the stylar canal or transmitting tissue

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Thomas M. Kon, Melanie A. Schupp, Hans E. Winzeler, and James R. Schupp

interaction of the intensity, duration, and rate of temperature change ( Wahid et al., 2007 ). As demonstrated with an ornamental lily, pollen tube growth in vivo was temporarily arrested with 10-s exposure to 45 °C ( Pierson et al., 1993 ). Unlike leaves and

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Dongmei Wei, Chao Gao, and Deyi Yuan

, 1967 ). In flowering plants, calcium plays crucial roles in regulating sexual reproduction processes such as pollen germination, pollen tube growth ( Cardenas et al., 2008 ; Franklin-Tong, 1999 ; Hepler, 1997 ; Heslop-Harrison, 1987 ; Kwack, 1967

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Qin Yang, Er Liu, Yan Fu, Fuqiang Yuan, Tingting Zhang, and Shu Peng

; Ohnishi et al., 2010 ; Saini et al., 2017 ; Yang et al., 2015a ). Complications can include abnormal meiosis ( Bita et al., 2011 ; Draeger and Moore, 2017 ; Ohnishi et al., 2010 ; Yang et al., 2015a ); accelerated pollen development and increased

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Thomas M. Kon, James R. Schupp, Keith S. Yoder, Leon D. Combs, and Melanie A. Schupp

blossoms. Common timings include 20%, 60%, 80%, 100%, or all full bloom, and single or multiple applications can be applied. Inconsistent blossom-thinning responses may be attributed to application timing because pollen tubes can reach the base of the style