Five greenhouse and two Geld experiments were conducted to evaluate tissue culture-propagated (TC) raspberry (Rubus idaeus cv. Heritage) sensitivity to preemergent herbicides. Plant performance was measured by plant vigor, above-ground fresh weight, root development, and primocane number. Simazine and oryzalin caused significant injury to newly planted TC raspberry plants in greenhouse and field experiments. The severity of injury was generally linear with respect to herbicide rate, but no appreciable differences in injury were observed between the granular and spray applications. Napropamide wettable powder caused some foliar injury, but plants recovered within one growing season and growth was equal or superior to the hand-weeded controls. The granular formulation of napropamide produced similar results, but did not cause the initial foliar burn. Pre-plant dipping of roots into a slurry of activated carbon did not prevent simazine or oryzalin injury, but injury was reduced when herbicide applications were delayed. Simazine applied 4 weeks after planting was not Injurious, and oqzalin applied 2 or 4 weeks after planting caused some foliar injury, hut no reduction in plant fresh weight. Delayed treatments of napropamide increased foliar injury. Herbicide tolerance of tissue-cultured plantlets appeared to be less than that of conventionally propagated plants. Chemical names used: N,N-diethyl-2-(1-napthalenyloxy)propanamide (napropamide), 4-(dipropylamino)-3,5-dinitrobenzenesulfonamide (oryzalin), 6-chloro-N,N'diethyl-1,3,5-triazine-2,4-diamine (simazine).
Joseph C. Neal, Marvin P. Pritts, and Andrew F. Senesac
Jacqueline K. Burns, Luis V. Pozo, Rongcai Yuan, and Brandon Hockema
Guanfacine and clonidine were combined with ethephon or metsulfuron-methyl in the spray tank and applied as foliar sprays to Citrus sinensis L. Osb. `Valencia', Citrus madurensis Loureiro (calamondin), and Prunus persica `Elberta' to determine their effects on leaf loss, fruit detachment force (FDF), immature fruit loss, and twig dieback. In `Valencia' orange, `Elberta' peach and calamondin, guanfacine and clonidine effectively reduced ethephon-induced defoliation in all three tree species, whereas only guanfacine was effective with metsulfuron-methyl applications in `Valencia'. The ability of ethephon to reduce FDF in `Valencia' was only minimally impaired by guanfacine but not impaired by clonidine. Both guanfacine and clonidine diminished the capacity of metsulfuron-methyl to reduce FDF. Guanfacine reduced immature fruit loss of `Valencia' caused by metsulfuron-methyl and reduced twig-dieback. Leaf loss was reduced whether guanfacine or clonidine were applied with ethephon, or 24 hours or 17 days before ethephon application. Guanfacine and clonidine reduced leaf loss induced by continuous exposure of potted calamondin trees to ethylene, and leaf loss was similar with guanfacine and 1-methylcyclopropene (1-MCP) treatments. In separate experiments, guanfacine and clonidine were unable to block ethylene perception in Arabidopsis seedlings and petunia flowers but promoted rooting in coleus and tomato vegetative cuttings, suggesting that these compounds have auxin-like activity. The results demonstrate the potential to enhance selectivity of abscission agents with guanfacine and clonidine. Chemical names used: 2-[(2,6-dichlorophenyl)amino]-2-imidazoline, clonidine; 5-chloro-3-methyl-4-nitro-pyrazole, CMN-P; [(2,6-dichlorophenyl)acetyl]guanidine, guanfacine; [(2-chloroethyl)phosphonic acid, ethephon; indole-3-butyric acid, IBA; 1-methylcyclopropene, 1-MCP.
James E. Ells and Ann E. McSay
Growth chamber tests demonstrated that alfalfa (Medicago sativa L.) residue is toxic to cucumber (Cucumis sativus L.) seed germination and seedling growth. Ground alfalfa roots at 0.5% (w/w, dry weight) inhibited germination when added to the growing medium. Alfalfa roots at 0.5% were also toxic to pregerminated cucumber seed. However, cucumber seedlings grew normally if this same medium was watered and incubated for >1 day before planting. Alfalfa particle size in media influenced cucumber performance, with the intermediate size (1 to 2 mm) being lethal to cucumbers.
T.L. Creger and F.J. Peryea
Phosphate fertilizer additions to soils containing lead arsenate (LA) pesticide residues can increase As volubility. Apricot (Prunus armeniaca L.) rootstock liners were grown in nondraining pots containing Burch loam soil that received a factorial treatment combination: 1) LA enrichment [no added LA (-LA), and LA added at 138 mg Pb/kg and 50 mg As/kg (+LA)]; 2) fertilizer type [monoammonium phosphate (MAP) and its sulfur analog ammonium hydrogen sulfate (AHS)]; and 3) fertilizer anion rate (0-26.1 mol/m3 soil). Measured response variables were soil salinity and pH, plant biomass, and plant As and Pb concentrations. Both MAP and AHS increased soil electrical conductivity (EC) and decreased soil pH, with AHS usually being more salinizing and acidifying than MAP was at equivalent rates. Adding LA reduced shoot and root mass and increased As and Pb concentration in shoots and roots. Shoot and root mass were inversely related to soil EC in the -LA soil but not in the +LA soil. Adding MAP increased shoot and root As concentration in the +LA soil, but adding AHS had no effect. Fertilizer type and rate did not influence shoot As concentration or root Pb concentration in the -LA soil or shoot Pb concentration in either the +LA or -LA soil. Adding AHS to the +LA soil increased root Pb concentration. These results are consistent with a P-enhanced solid-phase As release mechanism, which consequently increases plant uptake of soil As. Phosphate amendment had no effect on soil Pb phytoavailability.
Peter H. Dernoeden
Festuca species are being seeded into golf course roughs and natural or out-of-bound areas as alternative turfgrasses to replace perennial ryegrass (Lolium perenne L.) in the mid-Atlantic region. The tolerance of fine-leaf fescues to herbicides targeted for annual bluegrass (Poa annua L.) control, such as ethofumesate and prodiamine, is unknown. The objectives of this field study, therefore, were to assess the tolerance of `Rebel II' tall fescue (Festuca arundinacea Schreb.), and the fine-leaf fescue species `Reliant' hard fescue (Festuca longifolia Thuill.), `Jamestown II' Chewings fescue (Festuca rubra L. ssp. commutata Gaud.), and `MX 86' blue sheep fescue (Festuca glauca L.) to various rates, combinations, and times of application of ethofumesate and prodiamine. `Rebel II' was most tolerant of ethofumesate; however, sequential rates ≥0.84 + 0.84 kg·ha-1 reduced quality for 1 or more weeks and 2.24 + 2.24 kg·ha-1 caused unacceptable injury. Single applications of ethofumesate at rates of 0.56, 0.84, and 1.12 kg·ha-1, and sequential treatments of 0.56 + 0.56 and 0.84 + 0.84 kg·ha-1 reduced `Reliant' quality temporarily. Sequential treatments of high rates (i.e., 1.12 + 1.12 and 2.24 + 2.24 kg·ha-1), however, significantly reduced `Reliant' cover. `Jamestown II' was very sensitive to ethofumesate, but recovered from single applications of 0.56, 0.84, and 1.12 kg·ha-1; sequential applications (≥0.84 + 0.84 kg·ha-1) caused unacceptable injury, and rates ≥1.12 + 1.12 kg·ha-1 caused significant loss of cover. The cultivar MX 86 tolerated single applications of 0.56 to 2.24 kg·ha-1 of ethofumesate, but sequential treatments generally reduced quality to unacceptable levels. In one study, `Jamestown II' and `MX 86' were more severely injured when ethofumesate (1.12 or 2.24 kg·ha-1) was applied in October rather than in November. The fescues generally best tolerated a single, November application of ethofumesate at ≤1.12 kg·ha-1. Prodiamine (0.73 kg·ha-1) caused only short-term reductions in quality of `Jamestown II', but was generally noninjurious to the other fescues. Ethofumesate tank-mixed with prodiamine (0.84 + 0.36 or 1.12 + 0.73 kg·ha-1) elicited some short-term reduction in quality, but the level of injury was generally acceptable and injured fescues had recovered by spring. Chemical names used: [±]2-ethoxy-2,3-dihydro-3,3-dimethyl-5-benzofuranyl methanesulfonate (ethofumesate); N 3,N 3-di-n-propyl-2,4-dinitro-6-(trifluoromethyl)-m-phenylenediamine (prodiamine); S,S-dimethyl 2-(difluoromethyl)-4-(2-methylpropyl)-6-(trifluoromethyl)-3,5-pyridine-dicarbothioate (dithiopyr).
Patrick E. McCullough, Haibo Liu, and Lambert B. McCarty
Ethephon is an effective growth retardant for suppressing Poa annua (L.) seedheads in creeping bentgrass putting greens; however, ethylene induction may cause bentgrass leaf chlorosis, reduced rooting, and quality decline. Two greenhouse experiments investigated the effects of nitrogen (N) fertility and ethephon applications on `L-93' creeping bentgrass over 9 weeks. Ethephon was applied at 0, 3.8, and 7.6 kg·ha–1 a.i. per 3 weeks and N was applied at 4 and 8 kg·ha–1·week–1. Ethephon applications linearly reduced bentgrass quality on every weekly observation. Increased N rate to 8 kg·ha–1·week–1 improved turf quality about 10% to 20% and 10% to 30% from ethephon applied at 3.8 and 7.6 kg·ha–1 per 3 weeks, respectively. Increased N rate to 8 kg·ha–1·week–1 enhanced shoot growth 30% but reduced root mass and length 12% and 11%, respectively. After 9 weeks, ethephon reduced root length by about 30% and root mass about 35% at both rates. From nine weekly samples, ethephon reduced dry clipping yield 10% and 16% at 3.8 and 7.6 kg·ha–1 per 3 weeks, respectively. From 2 to 9 weeks after initial treatments, ethephon linearly increased leaf water content. Increasing N fertility effectively reduced bentgrass leaf chlorosis from ethephon; however, repeat applications of ethephon and increased N may restrict bentgrass root growth. Chemical names used: [(2-chloroethyl)phosphonic acid] (ethephon).
Hisashi Kato-Noguchi and Yukitoshi Tanaka
The allelopathic potential of Citrus junos Tanaka waste from food processing industry after juice extraction was investigated under laboratory conditions. C. junos waste powder inhibited the growth of roots and shoots of alfalfa (Medicago sativa L.), cress (Lepidium sativum L.), lettuce (Lactuca sativa L.), crabgrass (Digitaria sanguinalis L.), timothy (Phleum pratense L.) and ryegrass (Lolium multiflorum Lam.). Significant reductions in the growth of roots and shoots were observed as the powder concentration increased. The concentration of abscisic acid-β-d-glucopyranosyl ester (ABA-GE) in C. junos waste was determined to be 17.9 mg · kg–1 dry weight. Its concentration in C. junos waste appears to account mostly for the observed inhibition of tested plant seedlings. These results indicate that C. junos waste is allelopathic with potential for use in agriculture to suppress weed emergence, which should be investigated further in the field.
Olivia M. Lenahan and Matthew D. Whiting
Edmund J. Ogbuchiekwe, Milton E. McGiffen Jr., Joe Nunez, and Steven A. Fennimore
Preemergent and postemergent herbicides were evaluated in the Mediterranean climate of the southern San Joaquin Valley and the desert climate of the Imperial Valley from 1998 through 2000. Sixteen herbicide treatments were applied both as preemergence (PRE) and postemergence (POST) applications to carrot (Daucus carota L.). Carrot was generally more tolerant to PRE herbicide applications than to POST applications. Carrot was tolerant to PRE and POST imazamox and triflusulfuron at both locations. Carrot root losses due to herbicide were consistent with visual ratings. Treatments that injured carrot tops early in the growing season did not always reduce yield at the end of the season. PRE applications of imazamox and triflusulfuron did not affect carrot tops or the number or weight of marketable carrots. Carrots grown in the Imperial Valley and in the San Joaquin Valley were tolerant to PRE applications of carfentrazone, sulfentrazone, and imazamox. Results were similar for POST applications, although carfentrazone slightly injured carrot roots. PRE application of herbicides increased forked roots more than POST. Chemical names used: α, 2-dichloro-5-[4-(difluoromethyl)-4,5-dihydro-3-methyl-5-oxo-1H-1, 2,4-triazol-1-yl]-4-fluorobenzenepropanoic acid (carfentrazone); N-[2,4-dichloro-5-[4-(difluoromethyl)-4,5-dihydro-3-methyl-5-oxo-1H-1,2,4-triazol-1-yl]phenyl]me thanesulfonamide (sulfentrazone); N-(2 carbomethoxy-6-chlorophenyl)-5-ethoxy-7-fluoro (1,2,4) triazolo-[1, 5-c] pyrimidine-2-sulfonamide (cloransulam-methyl); 2-chloro-N-[(1-methyl-2-methoxy)ethyl]-N-(2,4-dimethyl-thein-3-yl)-acetamide (dimethenamid); (2-[4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl]-5-(methoxymethyl)-3-pyridinecarboxylic acid) (imazamox); 3-chloro-5-[[[[(4,6-dimethoxy-2-pyrimidinyl) amino] carbonyl] amino] sulfonyl]-1-methyl-1H-pyrazole-4-carboxylic acid (halosulfuron); N-[[(4,6-dimethoxy-2-pyrimidinyl)amino]carbonyl]-3-(ethylsulfonyl)-2-pyridinesulfonamide (rimsulfuron); (methyl 2[[[[[4-(dimethylamino)-6-[2,2,2-trifluoroethoxy)-1,3,5-triazin-2-yl] amino] carbonyl] amino] sulfonyl]-3-methylbenzoate) (triflusulfuron).
Monoammonium phosphate (MAP) is a popular starter fertilizer in Pacific Northwest tree fruit orchards; however, its use on soils contaminated with lead arsenate pesticide residues can enhance As solubility, thereby increasing As phytoavailability. `Fuji'/EMLA.26 apple trees (Malus ×domestica Borkh.) were planted in Mar. 1992 on a lead arsenate—contaminated Cashmont gravelly sandy loam soil (HCl-extractable soil As range: 60-222 mg·kg-1) using in-hole starter fertilizer application of either MAP or ammonium sulfate at equivalent N and anion rates. In ensuing years, all trees received identical applications of ammonium nitrate only. Relative trunk cross-sectional area was inversely related to soil As concentration in the year of planting but not in subsequent years, and was independent of starter fertilizer treatment. Leaf and fruit As were positively related to soil As in all years. Leaf As was initially higher in the MAP-treated trees; however, this effect diminished over time and disappeared by 1995. Fruit As was independent of starter fertilizer treatment, and was substantially lower than the tolerance established for As in fresh produce. The experimental results indicate that MAP starter fertilizer can increase soil As phytoavailability to apple trees grown under field conditions; however, the effects on tree growth and food safety are insignificant.