., 2008 ). The exact pollen germination percentage in different populations of M. sieversii remains unclear. Seed germination is another major aspect for plant reproduction. Relieving seed dormancy successfully is critical for fruit breeding. Evidence
Meiling Yang, Fang Li, Hong Long, Weiwei Yu, Xiuna Yan, Bin Liu, Yunxiu Zhang, Guorong Yan, and Wenqin Song
Yasser Ismail El-Nashar and Yaser Hassan Dewir
. from Jordan using seeds and in vitro culture techniques: Baseline information for a conservation perspective Afr. J. Biotechnol. 11 7684 7692 Ashkan, A. Jalal, M. 2013 Effect of salinity on seed germination and seedling vigor indices of two halophytic
Qi Zhang, Sheng Wang, and Kevin Rue
, alkaligrass ( Puccinellia spp.), a native of the prairies in the western United States and Canada, is highly recommended for home lawns under high saline stress. Harivandi et al. (1982) reported that 48% of Puccinellia spp. seeds germinated after a 15-d
Sherry Kitto and Jeanne Frett
Hexastylis shuttleworthii is a highly ornamental shade-tolerant evergreen herbaceous plant native to the southeastern U.S. that is difficult to propagate using traditional methods. Micropropagation would make possible the wider distribution of selected clones. Seeds were surface-sterilized and germinated in vitro. Seedling clones were maintained on a MS basal medium containing 1 mg/L BA and were subcultured monthly. Proliferation of clones 2 and 3, maintained on media supplemented with 1, 2.5 or 5 mg/L BA for 6 months, increased slightly with increasing BA concentration; however, proliferation decreased slightly over the experimental period. Rooting medium (perlite, vermiculite, MetroMix 510, Bacto Growers Mix) did not effect microcutting root production or subsequent plant survival. Microcuttings rooted in vitro (67% survival) generated more leaves compared to microcuttings rooted under humidity domes with mist in the greenhouse (8% survival). After rooting in vitro, multiple-shoot clumps (95%) survived better than individual shoots (29%) under greenhouse conditions. Plants were easily established when planted in raised beds in a lath house.
Michael Stein, Corina Serban, and Per McCord
Seed dormancy, specifically endodormancy, is a strong inhibitor of rapid germination in stone fruit seeds, including those of sweet cherry ( Prunus avium ). Endodormancy has been suggested to be a crucial process in seeds because it prevents
YanLing Zheng, GaoJuan Zhao, and HuanCheng Ma
. Optimum moisture contents for pea seeds stored at different temperatures Ann. Bot. (Lond.) 74 531 540 Yamazaki, J. Miyoshi, K. 2006 In vitro asymbiotic germination of immature seed and formation of protocorm by Cephalanthera falcata (Orchidaceae) Ann
Chad E. Finn, James J. Luby, Carl J. Rosen, and Peter D. Ascher
was supported, in part, by the U.S. Dept. of Agriculture under Specific Cooperative Agreement no. 58-32U4-7-047. The cost of publishing this paper was defrayed in part by the payment of page charges. Under postal regulations, this paper therefore must
X. Wang, J.T.A. Proctor, S. Krishna Raj, and P.K. Saxena
Ginseng is a very valuable agricultural species grown for its root, which contains pharmacologically active constituents. One limiting factor for expansion of ginseng production is an efficient method for mass propagation. Currently, seeding is the principal method of propagating ginseng, but the embryo of ginseng seeds at harvest is immature. A stratification schedule consisting of a cool-warm-cool temperature treatment over 18-22 months is required for embryo development and seed germination. An alternative for the efficient production of ginseng is mass propagation through the use of in vitro culture techniques. The objective of this work was to develop a highly efficient system for regeneration of ginseng. The efficacy of three auxins, viz. 2,4-D, NAA and dicamba, were compared for the induction of somatic embryogenesis in American ginseng. Somatic embryos formed on ginseng cotyledonary, zygotic embryo, and shoot explants after 8 weeks of induction by the auxins. Significantly more somatic embryos were induced by culture of any of the ginseng explants on media supplemented with 5 μmol·L-1 2,4-D than any other auxin treatment. Histological and SEM studies confirmed that the regenerants were somatic embryos. Somatic embryos germinated and developed into normal plants in 3-6 months. The development of a regeneration system for ginseng using somatic embryogenesis is a necessary first step for mass propagation and the improvement of American ginseng.
Jules Janick, Christiane Cabral Velho, and Anna Whipkey
Journal Paper no. 12,525 of the Purdue Univ. Agricultural Experiment Station. We thank William Carlson, Southern Forestry Res. Dept., Weyerhaeuser Corp., for providing seed. The cost of publishing this paper was defrayed in part by the payment of
Kimberly A. Pickens, Jan Wolf, James M. Affolter, and Hazel Y. Wetzstein
Many bromeliad species indigenous to the rain forests of Central and South America are threatened because of over-collection and habitat destruction. Studies were conducted to develop propagation protocols for Tillandsia eizii, a rare ornamental bromeliad of ceremonial significance to the Highland Maya communities in Chiapas, Mexico. We anticipate using in vitro propagation for the conservation of this species with the potential of utilizing bromeliads as an alternative and sustainable forest resource. Protocols were developed for the sterilization and germination of axenic seed. Seedling growth in vitro was assessed and outplanting studies were conducted. Media were evaluated to promote adventitious bud production in experiments using the plant growth regulators naphthaleneacetic acid and benzylaminopurine. Pulse time and duration, as well as the stage of seed development, had a marked effect on bud production. The effects of various potting media on plant growth and survival were assessed. A pure pine bark medium elicited over 95 percent survival. Plants exhibited a “tank-like” morphology characteristic of plants in the wild.