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Jacob George, Harsh Pal Bais, G.A. Ravishankar, and P. Manilal

Response surface methodology was utilized in statistical optimization of three quality factors (the number of multiple shoots, shoot length, and number of leaves) pertaining to regeneration of plantlets from leaf calli of Decalepis hamiltonii Wight. & Arn. (swallow root). The variables evaluated were the levels of sucrose, BA, and NAA each at two different concentrations. Response surfaces for shoot length and multiple shoot number were useful in achieving optimal levels of media constituents and in understanding their interactions, but response surfaces for number of leaves were not. The data indicate that sucrose, BA, and NAA levels may be manipulated to increase or decrease quality factors chosen. This approach may be useful in developing a micropropagation protocol for D. hamiltonii. Chemical names used: benzyladenine (BA); napthaleneacetic acid (NAA).

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Ping Song, Wanhee Kang, and Ellen B. Peffley

Regenerating calli of Allium fistulosum × A. cepa interspecific F1 hybrids were treated in vitro with colchicine. A factorial experiment (colchicine concentration × time) was used to recover tetraploids from calli treated with colchicine in vitro. Shoot production of regenerating calli following in vitro colchicine treatment decreased with increasing colchicine concentration and treatment time. Cytological analyses of root tip cells from regenerated plantlets showed that chromosomes of control plantlets (not treated with colchicine) were not doubled. Chromosomes of some plantlets regenerated from in vitro colchicine treated calli were doubled, resulting in tetraploids. Calli treated with 0.1 or 0.2% colchicine in BDS (Dunstan & Short, 1977) liquid medium for 48 or 72 hours yielded the highest number of tetraploid plantlets. These results demonstrate that in vitro colchicine treatment of regenerating calli of interspecific F1 hybrids is effective in recovering tetraploids.

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Xiaoling He, Susan C. Miyasaka, Yi Zou, Maureen M.M. Fitch, and Yun J. Zhu

successful system of genetic transformation must include a method for production of totipotent cells such as those found in callus tissues and then a method of regeneration of whole plants. Various combinations and levels of phytohormones have been reported

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Juanxu Liu, Min Deng, Richard J. Henny, Jianjun Chen, and Jiahua Xie

dish. Callus induction. To test the effects of growth regulator combinations on callus induction, a preliminary study was conducted by culturing bud, leaf, and stem explants on the basal medium supplemented with 4.4, 13.3, and 22.2 μM BA; 4

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Dinum Perera and Brian W. Trader

and axillary bud proliferation protocols of poinsettia ( De Langhe et al., 1974 ; Pickens et al., 2005 ; Roy and Jinnah, 2001 ). The role of cytokinins in developing caulogenetic callus from internodal explants was first reported in poinsettia ‘Paul

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Jin Cui, Juanxu Liu, Jianjun Chen, and Richard J. Henny

and concentrations listed in Tables 1 , 2 , and 3 . The medium was aliquoted to petri dishes (Fisher Scientific Inc., Pittsburgh, PA) at 20 mL each for callus initiation or to Magenta GA-7 vessels (PhytoTechnology Laboratories, Shawnee Mission, KS

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Samuel G. Obae, Hillar Klandorf, and Todd P. West

current study, we sought to: 1) evaluate how explants from the three root morphotypes (ML, BLB, and STK, hereafter referred to as ginseng lines) responded to in vitro callus induction and growth; 2) compare ginsenoside profiles and content among stock

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Mohsen Hesami and Mohammad Hosein Daneshvar

( Deshpande et al., 1998 ; Hassan et al., 2009 ; Siwach and Gill, 2011 , 2014 ) and only a single tissue culture and plant organogenesis protocol via callus phase ( Jaiswal and Narayan, 1985 ) exist, but all of those studies were based on mature explants

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Hamidou F. Sakhanokho, Kanniah Rajasekaran, and Rowena Y. Kelley

germinated within 35 to 40 d. Callus initiation and proliferation. Leaf explants were removed from 5- to 7-d-old in vitro germinated seedlings, cut into 3- to 4-mm segments, and transferred to 100 mm × 20-mm petri dishes containing the callus

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Song-jun Zeng, Zhi-lin Chen, Kun-lin Wu, Jian-xia Zhang, Cheng-ke Bai, Jaime A. Teixeira da Silva, and Jun Duan

) Yellowish callus mass developed from a protocorm. ( D ) PLBs developed from a callus mass. ( E ) Combination of shoot clusters, calli, and PLBs. ( F ) PLB-derived seedlings. ( G ) Transplanted seedlings on bark after growing in the greenhouse for 6 months