Search Results

using SSR and ISSR molecular markers. Materials and Methods Plant materials. We used two wild morphotypes of V. planifolia , “La Gran Lucha” and “Armadillo Chico,” both from Oaxaca, Mexico, as well as the cultivated morphotype “Mansa” of

the development of SSR markers in red raspberry ( Graham et al., 2002 , 2004 , 2006 ), it is now possible to examine specific loci for genetic polymorphisms and measure gene flow directly in the form of new alleles entering a population. Codominant

histogram produced by the ploidy analyzer. EST-SSR molecular analysis. Selected somatic tetraploid plants were further tested with molecular markers using EST-SSR method to determine whether they were auto- or allotetraploids. Genomic DNA of recovered

. Microsatellite markers, or SSRs, are an efficient method to assess the genetic diversity and population structure of plant populations [ Powell et al. (1996) ; reviewed in Varshney et al. (2005) ; Wang et al. (2009 )], and have proved useful for guiding

a , 2012 , 2013 ; Mady et al., 2013 ; Paris et al., 2003 ). SSR (microsatellites) are very popular molecular markers because they are highly informative, multiallelic, polymerase chain reaction (PCR)-based, and codominant. In this study, SSRs

this study was to use SSR markers to assess genetic relationships in a diverse collection of ornamental Prunus germplasm at the USNA. This genetic information has multiple applications: 1) to gain insight into taxonomic relationships in this group, 2

3.1.2 (Applied Biosystems). Genotyper software Version 2.5.2 (Applied Biosystems) was used to process and view GeneScan-sized peaks. Table 2. Simple sequence repeat (SSR) marker name, linkage group ( Jung et al., 2008 ), annealing temperature and

using simple sequence repeat length polymorphisms (SSRs). Subsequently, codominant simple sequence repeat markers were used to detect genetic diversity in watermelon ( Patcharin et al., 2011 ). Recently, two types of molecular markers (RAPD and SSR) were

and biochemical analyses ( Chen and Zhou, 2005 ; Chen et al., 2005 ; Jin et al., 2014 ) and SSR markers ( Fang et al., 2012 ; Tan et al., 2015 ; Yao et al., 2012a ) have been frequently used to investigate the genetic diversity of tea germplasm

, require less DNA, and can often be multiplexed, resulting in increased savings to researchers. Microsatellite or simple sequence repeat (SSR) markers originally isolated from Arabidopsis thaliana (L.) Heynh. and Brassica napus L. libraries have been