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Salih Kafkas, Yıldız Doğan, Ali Sabır, Ali Turan, and Hasbi Seker

Random amplified polymorphic DNA (RAPD) analysis of long-term cultured hybrid hazelnut HortScience 39 1079 1082 Okay, A.N. 1999 Allahverdi Findik Cesidinin Tanimlanmasi, Tarim ve Koyisleri Bakanligi, Tarimsal Arastirmalar Genel Mudurlugu Findik Arastirma

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Fachun Guan, Shiping Wang, Rongqin Li, Mu Peng, and Fanjuan Meng

contrast, three individuals (1, 2, and 3) belonging to the same altitude were separated into different groups. Fig. 8. Unweighted pair group method with arithmetic mean clustering based on genetic distance from random amplified polymorphic DNA data of 14 P

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Ghazal Baziar, Moslem Jafari, Mansoureh Sadat Sharifi Noori, and Samira Samarfard

, detained in sterilized distilled water for 3 min, and visualized under ultraviolet light and photographed. Table 4. Randomly amplified polymorphic DNA polymorphism and amplification pattern in Ficus carica . Data analysis. The digital image files were

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Amnon Levi and Claude E. Thomas

distributed on 19 linkage groups (10 large and nine small linkage groups) and covers a genetic distance of 1976 cM with an average distance of 5.8 cM between two markers. The map consists mostly of randomly amplified polymorphic DNA (RAPD), intersimple

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Caihong Wang, Yike Tian, Emily J. Buck, Susan E. Gardiner, Hongyi Dai, and Yanli Jia

). The seedling trees are 4 years old. Random amplified polymorphic DNA marker analysis. Polymerase chain reaction (PCR) amplification was performed in a 25-μL volume containing 50 ng of genomic DNA, 2.5 μL 10× PCR buffer, 1.8 m m magnesium chloride

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Kang Hee Cho, Jung Ho Noh, Seo Jun Park, Se Hee Kim, Dae-Hyun Kim, and Jae An Chun

conditions. Among the polymorphic bands, 50 amplified DNA bands were selected as RAPD markers for identifying the cultivars and were used in subsequent analyses. Table 2. Randomly amplified polymorphic DNA primers used in this study, their sequences, and

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Thomas J. Molnar, David E. Zaurov, Joseph C. Goffreda, and Shawn A. Mehlenbacher

, 1991b ). Recently, breeding for resistance has been facilitated by the identification of several random amplified polymorphic DNA (RAPD) markers tightly linked to the ‘Gasaway’ gene ( Davis and Mehlenbacher, 1997 ; Mehlenbacher et al., 2004 ). Marker

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Xi Li, Dongqin Tang, and Yimin Shi

extension at 72 °C for 10 min. After the amplification, the samples were run on 1.2% agarose gel containing 0.5 µg·mL −1 of ethidium bromide. The electrophoretic separation took ≈1 h at 80 V. Table 1. Sequence of the 10 random amplified polymorphic DNA

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Kai-Ge Zhao, Ming-Qin Zhou, Long-Qing Chen, Donglin Zhang, and Gituru Wahiti Robert

markers (ISSR) and random amplified polymorphic DNA markers (RAPD) are two commonly used methods, mainly because they are simple to execute, are low cost, and require neither sequence information nor prior genetic studies ( Williams et al., 1990

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Vidyasagar R. Sathuvalli, Shawn A. Mehlenbacher, and David C. Smith

resistance alleles would greatly facilitate the development of new cultivars. Random amplified polymorphic DNA and SSR markers linked to EFB resistance have been identified for three sources: ‘Gasaway’, ‘Ratoli’ from Spain, and OSU 759.010 from the Republic