Light and temperature responses of whole-plant CO2 exchange were determined for two cultivars of Angelonia angustifolia Benth., `AngelMist Purple Stripe' and `AngelMist Deep Plum'. Whole crop net photosynthesis (Pnet) of `AngelMist Purple Stripe' and `AngelMist Deep Plum' were measured at eight temperatures, ranging from 17 to 42 °C. Pnet for both cultivars increased from 17 to ≈20 °C, and then decreased as temperature increased further. Optimal temperatures for Pnet of `AngelMist Purple Stripe' and `AngelMist Deep Plum' were 20.8 and 19.8 °C, respectively. There was no significant difference between the two cultivars, irrespective of temperature. The Q10 (the relative increase with a 10 °C increase in temperature) for Pnet of both cultivars decreased over the entire temperature range. Dark respiration (Rdark) of both cultivars showed a similar linear increase as temperature increased. As photosynthetic photon flux (PPF) increased from 0 to 600 μmol·m-2·s-1, Pnet of both cultivars increased. Light saturation was not yet reached at 600 μmol·m-2·s-1. The light compensation point occurred at 69 μmol·m-2·s-1 for `AngelMist Purple Stripe' and at 89 μmol·m-2·s-1 for `AngelMist Deep Plum'. The lower light saturation point of `AngelMist Purple Stripe' was the result of a higher quantum yield (0.037 mol·mol-1 for `AngelMist Purple Stripe' and 0.026 mol·mol-1 for `AngelMist Deep Plum'). The difference in quantum yield between the two cultivars may explain the faster growth habit of `AngelMist Purple Stripe'.
Amanda M. Miller, Marc W. van Iersel, and Allan M. Armitage
M.P. Kaczperski, W. H. Carlson, and M.G. Karlsson
Petunia × hybrids `Snow Cloud' plants were grown under 25 temperature combinations ranging from 10 to 30C and at photosynthetic photon flux levels of 100 or 200 μmol·s-1·m-2 (6.5 and 13 mol·day-1·m-2, respectively). Days to flower-was a quadratic function of average temperature, with 25C being the optimum temperature for minimal tire-e to flower at 200 μmol·s-1·m-2. Plant height increased “linearly and average internode length increased quadratically as day temperature increased. The number of lateral shoots decreased quadratically as average temperature increased, and the average length of each shoot decreased quadratically as day temperature increased.
P.C. Andersen, J.G. Norcini, and G.W. Knox
Leaf physiology and plant growth of Rhododendron × `Pink Ruffles' were compared under conditions of 100% sun and under polyethylene shadecloth with specifications of 69%, 47%, and 29% light transmittance. Net CO2 assimilation (A) and stomatal conductance to water vapor (gs) were often reduced for plants in the 100% sun regime, although few differences existed among the 69%, 47%, and 29% sun treatments. Stomatal conductance was very sensitive to leaf to air vapor pressure deficits (VPD), as evidenced by an 85% increase in gs with a decrease in VPD from 3.2 to 2.2 kPa. Light response curves established for plants after 54 days of exposure to 100% and 29% sun were similar, although A was consistently higher at all levels of photosynthetic photon flux for plants in the 29% sun regime. Maximum A was ≈5 and 6 μmol·m-2·s-1 for 100% and 29% sun-grown plants, respectively; light saturation occurred at ≈ 800 μmol·m-2·s-1 Midday relative leaf water content and leaf water potential were not affected by sun regime. The plant growth index decreased with increasing light level. Leaf, stem, and root dry weights; total leaf number and dry weight; total and individual leaf area; dry weight per leaf; and leaf chlorophyll concentration were reduced in 100% sun, yet few differences existed among the 69%, 47%, and 29% sun treatments. Shoot: root ratio and specific leaf weight were proportional to light level. Plants grown in the 100% sun regime were chlorotic and dwarfed, and plants in 29% sun were not sufficiently compact. One year after transplanting to the field under 100% sun, plants of all treatments were chlorotic and failed to grow.
K.I. Theron and G. Jacobs
Large Nerine bowdenii bulbs (>14 cm in circumference) were exposed to low ligbt intensities for different periods during two successive growing seasons. The flowering percentage and number of florets in the current season's inflorescence were recorded at anthesis. Small and large bulbs were subjected to continual defoliation starting at different times during the growing season. Bulbs were dissected at planting (26 Sept. 1992) and on 12 Jan. 1993 (nondefoliated control bulbs) to determine growth and developmental stage. At anthesis, inflorescences were harvested and the florets per inflorescence were counted. After anthesis in the fall, all bulbs were dissected and the following variables recorded: 1) percentage flowering, quiescence, or abortion of the current season's inflorescence; 2) developmental stage of quiescent inflorescences; 3) number of florets in the outermost inflorescence; 4) developmental stage of the innermost inflorescence; 5) number of leaves or leaf bases in each growth unit; 6) number of daughter bulbs; and 7) dry weight of new leaf bases. There were three reasons for nonflowering of the bulbs, viz., failure to initiate an inflorescence, inflorescences remaining quiescent, and inflorescence abortion. Individual florets that had not reached stage “Late G” (gynoecium elongated, carpels fused) at the start of rapid inflorescence elongation aborted. The more florets that aborted, the greater the probability that the entire inflorescence aborted. The inflorescence was more vulnerable to stress during the first half of the growing season due to its relatively weak position in the hierarchy of sinks within the bulb.
Usha R. Palaniswamy, Richard J. McAvoy, and Bernard B. Bible
Purslane (Portulaca oleracea L.) is an excellent source of the essential fatty acid α-linolenic acid (LNA) but little is known of the effects of cultural conditions on LNA concentration. Purslane seedlings were grown under an instantaneous photosynthetic photon flux [PPF (400 to 700 nm)] of 299 or 455 μmol·m-2·s-1 for a daily duration of either 8, 12, 16, or 20 hours. Thus, plants were exposed to a daily PPF of 8.6, 12.9, 17.2, or 21.5 mol·m-2·d-1 in the low PPF treatment (299 μmol.m-2.s-1) and 13.1, 19.7, 26.2, or 32.8 mol·m-2·d-1 in the high PPF treatment (455 μmol·m-2·s-1). Plants in all treatments received a 20-hour photoperiod by providing ≈5 μmol·m-2·s-1 from incandescent lamps starting at the end of the photosynthetic light period. At low PPF, purslane grown under a 16 hour PPF duration produced the highest concentrations of total fatty acids (TFA) and LNA per unit leaf dry weight (DW), but at high PPF, concentrations of these compounds were highest under 8 and 12 hour PPF duration. Trend analysis indicated that maximum TFA and LNA concentrations occurred with a daily PPF of 14.1 and 17.2 mol·m-2·d-1, respectively; and in the thylakoids, protein, chlorophyll, and LNA concentrations peaked at a PPF of 21.8, 19.9, and 16.1 mol·m-2·d-1, respectively. LNA as a percentage of TFA was unaffected by treatment. Shoot DW increased with PPF up to the highest PPF exposure of 32.8 mol·m-2·d-1.
L. Carl Greve, Gale McGranahan, Janine Hasey, Ronald Snyder, Kathy Kelly, David Goldhamer, and John M. Labavitch
The variation in polyunsaturated fatty acid content of walnut (Juglans regia L.) oils was determined by analysis of samples isolated from specimens growing in four germplasm collections [California (55 cultivars), Washington (64 seedlings), China (12 cultivars), and France (20 cultivars)]. In addition, the impact of within-state geographic differences on oil composition was examined by comparing samples from three California cultivars (`Ashley', `Hartley', and `Franquette') grown in three locations. Local environmental effects on oil composition of `Chico' were also examined by comparing 1) samples collected from shaded and sun-exposed locations of the same trees and 2) samples collected from trees subjected to three irrigation regimes. Polyunsaturated fatty acid content, as a percentage of total fatty acids, ranged from 47.2% in nuts from PI 142323 from France to 81.0% in `Ashley' from California. However, our data indicate that environment, genotype, nut maturity, and their interactions all contribute significantly to variation in the degree of unsaturation of walnut oil.
William R. Graves and Hongyi Zhang
Air temperature and photosynthetically active radiation (PAR) effects on relative water content (RWC), rooting percentage, root count, and root mass of unmisted, subirrigated stem cuttings of two taxa were determined. Leaf RWC of `Charm' chrysanthemum [Dendranthema ×grandiflorum (Ramat.) Kitamura] decreased until roots initiated and then increased, was lower for cuttings at 23 °C photoperiod/14 °C dark than for cuttings at 31 °C photoperiod/22 °C dark, and was lower at 193 than at 69 μmol·m–2·s–1 PAR. Neither temperature nor PAR affected leaf RWC of `Dollar Princess' fuchsia (Fuchsia ×hybrida Hort. ex Vilm.), which increased linearly before and after root initiation. Rooting percentage and root count were higher with photoperiods at 31 °C than at 23 °C for chrysanthemum after 7 days and for fuchsia after 10 days. Although all cuttings of both taxa had rooted after 14 days, root dry mass was higher with photoperiods at 31 °C than at 23 °C regardless of PAR for fuchsia and at 69 μmol·m–2·s–1 PAR for chrysanthemum. Propagators wishing to use subirrigation instead of mist, fog, or enclosure can minimize the decline in leaf RWC before root initiation and increase the number and dry mass of roots of chrysanthemum by using 69 μmol·m–2·s–1 PAR and a 31 °C photoperiod/22 °C dark cycle. Root dry mass of fuchsia also can be increased by the use of high temperature, but differences in rooting were independent of changes in leaf RWC.
Mark G. Lefsrud, Dean A. Kopsell, Robert M. Augé, and A.J. Both
Consumption of fruit and vegetable crops rich in lutein and β-carotene carotenoids is associated with reduced risk of cancers and aging eye diseases. Kale (Brassica oleracea L. var. acephala D.C.) ranks highest for lutein concentrations and is an excellent source of dietary carotenoids. Kale plants were grown under varied photoperiods to determine changes in the accumulation of fresh and dry biomass, chlorophyll a and b, and lutein and β-carotene carotenoids. The plants were cultured in a controlled environment using nutrient solutions under photoperiod treatments of 6, 12, 16, or 24 hours (continuous). Fresh and dry mass production increased linearly as photoperiod increased, reaching a maximum under the 24-hour photoperiod. Maximum accumulation of lutein, β-carotene, and chlorophyll b occurred under the 24-h photoperiod at 13.5, 10.4, and 58.6 mg/100 g fresh mass, respectively. However, maximum chlorophyll a (235.1 mg/100 g fresh mass) occurred under the 12-hour photoperiod. When β-carotene and lutein were measured on a dry mass basis, the maximum accumulation was shifted to the 16-hour photoperiod. An increase in photoperiod resulted in increased pigment accumulation, but maximum concentrations of pigments were not correlated with maximum biomass production.
Ryan M. Warner and John E. Erwin
Thirty-six Hibiscus L. species were grown for 20 weeks under three lighting treatments at 15, 20, or 25 ± 1.5 °C air temperature to identify flowering requirements for each species. In addition, species were subjectively evaluated to identify those species with potential ornamental significance based on flower characteristics and plant form. Lighting treatments were 9 hour ambient light (St. Paul, Minn., November to May, 45 °N), ambient light plus a night interruption using incandescent lamps (2 μmol·m-2·s-1; 2200 to 0200 hr), or ambient light plus 24-hour supplemental lighting from high-pressure sodium lamps (100 μmol·m-2·s-1). Five day-neutral, six obligate short-day, six facultative short-day, three obligate long-day, and one facultative long-day species were identified. Fifteen species did not flower. Temperature and lighting treatments interacted to affect leaf number below the first flower and/or flower diameter on some species. Hibiscus acetosella Welw. ex Hiern, H. cisplatinus St.-Hil., H. radiatus Cav., and H. trionum L. were selected as potential new commercially significant ornamental species.
Martin P.N. Gent
were based on the weight of plants at 2, 4, or 6 weeks. The data were regressed against normalized irradiance, daily light integral divided by leaf area index. Normalized and integrated irradiance were equal if the index was less than one. The dry