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Jin Cui, Juanxu Liu, Jianjun Chen, and Richard J. Henny

concentrations of growth regulators showed lower shoot formation frequencies (60% or less) and lower shoot numbers (19 or less) than the aforementioned three combinations. Rooting, acclimatization, and greenhouse production. Few adventitious shoots produced roots

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Maria Papafotiou and Aekaterini N. Martini

potential of each culture by showing the number of explants that the culture could provide for a subsequent subculture. Root induction and acclimatization. Microshoots, 1.0–2.0 cm long, produced in the first culture established from in vitro-grown seedlings

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Georgia Vlachou, Maria Papafotiou, and Konstantinos F. Bertsouklis

, microshoot clusters produced on multiplication media were put on Hf-half-strength MS medium for rooting. Ex vitro acclimatization and establishment Rooted plantlets were transferred into 500-mL containers (eight plantlets per container), on a mixture of peat

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Mohsen Hesami and Mohammad Hosein Daneshvar

induction, number of roots per shoot, and the root length were recorded after 5 weeks. Acclimatization and transplantation. The plantlets were removed from the culture tubes within 4 weeks after transferring to MS medium and washed several times with

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Yujie Yang, Donglin Zhang, Zhihui Li, Xiaoling Jin, and Jinying Dong

(centimeters) were recorded. Transplanting and acclimatization. Four weeks after transferring to the light conditions, seedlings with at least two true leaves were transplanted to a tray with 32 cells (6.5 × 6.5 × 9 cm 3 ) with a 1:1 mixture of AERO

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Margaret W. Kirika, Jane W. Kahia, Lucien N. Diby, Eliud M. Njagi, Colombe Dadjo, and Christophe Kouame

d, and ( D ) 28 d after culture. Acclimatization. The regenerated plantlets ( Fig. 5 ) were planted in a weaning pot and the two holes on the cover were kept closed for the first 2 week and eventually fully opened after 1 month ( Fig. 6A–C ). At the

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Gregory T. Browne, Charles A. Leslie, Joseph A. Grant, Ravindra G. Bhat, Leigh S. Schmidt, Wesley P. Hackett, Daniel A. Kluepfel, Reid Robinson, and Gale H. McGranahan

fir bark (by volume) potting soil in containers (Ray Leach Cone-tainers; Stuewe and Sons, Tangent, OR) and then acclimatized to ambient greenhouse conditions by gradually reducing ambient humidity. Established plants were repotted to larger 0.5-L

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Jeffrey Adelberg, Maria Delgado, and Jeffrey Tomkins

( Hemerocallis spp.) using AFLP markers Theor. Appl. Genet. 102 489 496 Ziv, M. 1992 The use of growth retardants for the regulation and acclimatization of in vitro plants 809 817 Karsen C. Van Loon L

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Hak-Tae Lim, Haeng-Soon Lee, and Tage Eriksson

Plant regeneration of ginseng has been known to be difficult, and there are a few reports on plant regeneration of ginseng via somatic embryogenesis. In vitro flowering has, however, been one of the major drawbacks in these regeneration systems in which BA and GA3 were included in germination and shoot multiplication media. Multiplication of adventitious shoots from a single somatic embryo, abnormal morphology, and vitrified shoots were also observed. All these facts have made successful acclimatization of ginseng plantlets difficult. The purposes of this study were 1) to establish the plant regeneration system via organogenesis, 2) to improve normal plant regeneration via somatic embryogenesis, 3) to improve the efficiency of plant regeneration from protoplast culture, 4) to understand the acclimatization process, 5) to develop effective genetic transformation protocol. Data in relation with all these studies are presented in detail.

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M.L. Arrebola, O. Socorro, A. Barceló-Muñoz, E. Simón-Pérez, and Fernando Pliego-Alfaro

A micropropagation procedure for juvenile and adult savory (Satureja obovata Lag.) explants is described. Pretreatment of the nutlets with gibberellic acid (0.57 mm) did not improve in vitro germination. Optimum shoot proliferation of juvenile and adult material was obtained on medium containing 2.22 μm N6-benzyladenine. Rooting and acclimatization of juvenile shoots were accomplished in vivo, while adult shoots were rooted in vitro after 3 days of exposure to 4.92 μm indole-3-butyric acid followed by subsequent transfer to auxin-free medium. More than 95% survival of adult rooted plants was obtained during the acclimatization phase. Chemical names used: gibberellic acid (GA3); N6-benzyladenine (BA); indole-3-butyric acid (IBA); isopentenyladenine (2iP).