was carried out on a 250 × 4.6-mm, 5-μm column (Diamonsil C18; Dikma Technologies, Foothill Ranch, CA). Detection was operated at 214-nm wavelength with 0.1 mmol·L −1 KH 2 PO 4 (pH = 2.6) as mobile phase passed through a 0.45-μm membrane filter. The
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Xiaoning Li, Xiaoyan Sun, Guangyang Wang, Erick Amombo, Xiuwen Zhou, Zhaohong Du, Yinkun Zhang, Yan Xie, and Jinmin Fu
Yi Gong, Ronald B. Pegg, Adrian L. Kerrihard, Brad E. Lewis, and Richard J. Heerema
(pH 2.0, using 6 M HCl). With a separatory funnel, the free phenolic compounds were extracted with diethyl ether (5 × 20-mL portions). The extractant was composed of acetone, water, and acetic acid. The organic phase (acetone) was removed using the
Timothy W. Coolong and William M. Randle
remaining pellet was again resuspended in 40 mL of HCl, pH 2.5, and incubated for 90 min at 60 °C. The extract was centrifuged and filtered to obtain the acid-soluble pectin (ASP). The remaining pellet was resuspended in 40 mL of 0.05 m NaOH and incubated
Derek W. Barchenger, John R. Clark, Renee T. Threlfall, Luke R. Howard, and Cindi R. Brownmiller
) with an automated titrimeter and electrode standardized to pH 2.0, 4.0, 7.0, and 10.0 buffers. Titratable acidity was determined using 6 g of juice diluted with 50 mL of deionized, degassed water by titration of 0.1 N sodium hydroxide to an endpoint of
Lixiang Miao, Yuchao Zhang, Xiaofang Yang, Jinping Xiao, Huiqin Zhang, Ming Jiang, Zuofa Zhang, Yuezhi Wang, and Guihua Jiang
Detector set at 210 nm using an X-bridge C18 column (4.6 × 250 mm, 5 μm, Waters Corporation) operated at 30 °C. The elution solvent was 0.01 mol·L −1 sulfuric acid (pH 2.6) passed at a rate of 0.5 mL·min −1 . The quantity and type of organic acids were
Ryan W. Dickson and Paul R. Fisher
= 0.8780 × Δ pH 3 + 1.3369 × Δ pH 2 + 9.9856 × Δ pH − 0.2569 [1] Plant growth was measured as root and shoot dry weight gain during the experiment. Root and shoot tissue from seedlings and from final replicates was oven-dried for 48 h
Baoxin Chang, Benjamin Wherley, Jacqueline Aitkenhead-Peterson, Nadezda Ojeda, Charles Fontanier, and Philip Dwyer
pH using a pH meter (Model 215; Denver Instrument, Bohemia, NY). We extracted P, K, Ca, Mg, Na, and S using the Mehlich III extractant (a dilute acid-fluoride-EDTA solution of pH 2.5 that consists of 0.2 N CH 3 -COOH, 0.25 N NH 4 NO 3 , 0.015 N NH 4 F
Konstantinos G. Batziakas, Shehbaz Singh, Kanwal Ayub, Qing Kang, Jeffrey K. Brecht, Cary L. Rivard, and Eleni D. Pliakoni
of 5 m m potassium phosphate monobasic (KH 2 PO 4 ), pH 2.65, with 0.1% of formic acid (solution A) and methanol with 0.1% of formic acid (solution B). The linear gradient of the mobile phase was programmed as follows: 5% to 15% B for 1 min, followed
Dipayan Sarkar, Prasanta C. Bhowmik, Young-In-Kwon, and Kalidas Shetty
20 m m potassium phosphate (pH 2.5 by phosphoric acid) at a flow rate of 1 mL/min and were detected at 210 nm. L-Proline (Sigma) dissolved in the 20 mm potassium phosphate solution was used to calibrate the standard curve. The amount of proline in
Rong Zhang, Zhubing Yan, Yikun Wang, Xuesen Chen, Chengmiao Yin, and Zhiquan Mao
. The chromatographic column (3 μm, 150 × 3 mm) was an Acclaim 120 C18 (Dionex, Sunnyvale, CA), and the column temperature was 30 °C. Mobile phase A was acetonitrile, mobile phase B was water (adjusted to pH 2.6 with acetic acid), and the flow rate was 0