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María Engracia Guerra, Ana Wünsch, Margarita López-Corrales, and Javier Rodrigo

., 1996 ) in polystyrene petri dishes (60 × 10 mm). Pollen was germinated for 24 h at 20 °C and then frozen at –20 °C to arrest pollen germination. Preparations were thawed during 24 h at 4 °C, stained with 1% (v/v) aniline blue in 0.1 n K 3 PO 4 to

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Huan Xiong, Ping Chen, Zhoujun Zhu, Ya Chen, Feng Zou, and Deyi Yuan

heterosis in this species. Male sterility in plants is defined by the loss of function or degeneration of male organs resulting from genetic or physiological factors, and these plants cannot produce normal pollen or anthers ( Yu et al., 2010 ). Male

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Yang Hu, Chao Gao, Quanen Deng, Jie Qiu, Hongli Wei, Lu Yang, Jiajun Xie, and Desheng Liao

cells underwent mitosis to form pollen mother cells that were surrounded by callose. ( B4 ) Callose underwent the first degradation, which was manifested by light staining, and it released single pollen mother cells for meiosis. ( C1 ) The pollen mother

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Bandara Gajanayake, Brian W. Trader, K. Raja Reddy, and Richard L. Harkess

grains were collected as described for the PG procedure from 30 plants in each cultivar between 900 and 1000 hr . Pollen viability was tested using 2.5% concentration of 2, 3, 5-triphenyl tetrazolium chloride (TTC) stain in deionized water as described

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Fengxia Shao, Sen Wang, Zhiming Liu, Rongyan Hong, and Tianjiao Zhao

pollen, embryo sac, embryo, and endosperm developmental process could result in seed abortion ( Liang et al., 2005 ). The embryological mechanism of seed abortion is divided into four aspects: male sterility, female abortion, pollination, and

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Lisa Alexander

) with an Olympus Q Color 5 digital camera for image capture. Measurements and counts were made using Q-Capture Pro 7 software. Pollen stainability. Six diploid and six triploid plants were used to estimate pollen viability. Fresh pollen from a single

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Wei Deng, Yunling Xie, and Yilan Qiu

Cell. Dev. Biol. Plant 54 145 153 Heslop-Harrison, J. Heslop-Harrison, Y. 1970 Evaluation of pollen viability by enzymatically-induced fluorescence; intracellular hydrolysis of fluorescein diacetate Stain Technol. 45 115 120 Imre, K. Kristóf, Z. 1999

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Hsuan Chen, Lan Xue, Tong Li, and Ryan N. Contreras

). Based on acetocarmine staining, F 1 hybrids had 65% to 88% stainable pollen, which was slightly lower than that of the parents, which had 90% to 99% stainable pollen ( van Laere et al., 2009 ); however, the in vivo pollination success rate and male

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Wagner A. Vendrame, Virginia S. Carvalho, José M.M. Dias, and Ian Maguire

and color patterns. Furthermore, their relatively short production cycle from seedling to a blooming plant provides a highly valuable and desirable characteristic for commercial large-scale production. Pollen storage is of great importance for plant

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Sergio Melgar and Michael J. Havey

harvested from field-grown plants in Summer 2008 and stained with acetocarmine ( Yamamoto et al., 1997 ). Pollen was observed under the microscope and was classified as viable if there was a pink cytoplasm, elliptical shape, and two red-staining nuclei