Ethephon [2-(chloroethyl) phoshonic acid] is used widely to maximize the yield of ripe tomato fruits. However, ethephon causes rapid and extensive defoliation, overripening, and promotes sunscald damage to the fruit. Recent studies from our laboratory have provided evidence that lysophosphatidylethanolamine (LPE) can reduce leaf senescence. We investigated the potential use of LPE to reduce damaging effect of ethephon on tomato foliage and influence on the activity of phospholipase D (PLD). Disruption of membrane integrity has been suggested as a primary cause of senescence in plants. PLD is known to be a key enzyme, which initiates the selective degradation of membrane phospholipids in senescing tissues. Two-month-old tomato plants (`Mountain Spring') grown in greenhouse condition were sprayed with water, 200 ppm LPE, and 1000 ppm ethephon. In addition, LPE spray prior to ethephon or mixture with ethephon were also tested. Leaves were sampled after 0, 2, 5, 24, 72, and 168 h of spray application, for PLD activity measurements. Spray of LPE prior to ethephon spray or inclusion of LPE in the ethephon spray reduced foliar injury by ethephon. Activity of soluble PLD was increased dramatically in leaves sprayed with ethephon initially and than dropped by 7 days. We also found that LPE-treated leaves had lower PLD activity than the ethephon-treated leaves. Plants treated with LPE-ethephon mixture also showed significantly lower PLD activity. These results suggest that LPE treatments mitigate ethephon injury to tomato plants. Furthermore, it appears that this mitigation involves modulation of the activity of PLD.
Mustafa Ozgen, Jiwan P. Palta, and Stephen B. Ryu
Steven E. Newman, Michael J. Roll, and Ronald J. Harkrader
There are many naturally occurring substances that have the potential to be adapted to modern pest control chemistry. Azadirachtin, an insect growth regulator, is one such naturally occurring compound that has been widely accepted in insect pest management. Quartenary benzophenanthridine alkaloids (QBAs) are known to be effective in the control of crop damaging fungal diseases. QBAs can be isolated from plants in the Papaveraceae. Extracts of Macleaya cordata, a species rich in QBAs, were formulated at 150 mg·L–1 QBA for spray application to greenhouse roses infected with Sphaerotheca pannosa var. rosae (powdery mildew). The QBA formulation was applied at 10-day intervals. Copper sulfate pentahydrate (Phyton27), piperalin (Pipron), and fenarimol (Rubigan) were also applied to mildew infected plants within the same greenhouse at their respective label rates for comparison. One day after treatment, the mildew infection was reduced 50% by QBA, whereas fenarimol, copper sulfate pentahydrate, and piperalin reduced the infection 50%, 75%, and 80%, respectively. Nine days after application, the mildew infection of QBA treated plants was less than 5% of the leaflet surface area. QBAs have the potential to be developed as a biorational fungicide for greenhouse use with both fungicidal and fungistatic activity.
Michael J. Roll, Steven E. Newman, and Ronald J. Harkrader
A formulation of quaternary benzophenathridine alkaloids (QBA) was combined with piperalin as a tank mix. The QBA was applied at 150 mg/L and piperalin, at the labeled rate, was applied as a spray application to greenhouse roses infected with Sphaerotheca pannosa var. rosae (powdery mildew). Copper sulfate pentahydrate and fenarimol were also applied to mildew-infected plants within the same greenhouse at their respective label rates for comparison. Initial infection for the QBA/piperalin combination spray was 45% of the leaflet surface area, 3 days after application the infection was reduced to 10%, 6 days after application infection was reduced to 5%, and 14 days after application the infection remained at 5%. Initial infection for a QBA application without piperalin was 25% of the leaflet surface area. Three days after application, the infection was reduced to 15%; 6 days after application the infection remained at 15%; and 14 days after application, the infection was reduced to 10%. The data reveals that the QBA/piperalin combination gives a short-term as well as a long-term fungicidal and fungistatic activity.
J.H. Lieth, L.R. Oki, P. Ng, M.C. Garcia-Navarro, S.H. Kim, and L.-Y. Li
Daminozide is a growth retardant used in potted plant production as a foliar spray to inhibit shoot elongation. It has its greatest inhibitory effect immediately after application, becoming less pronounced thereafter; continued retardation is accomplished by reapplication at 7to 14-day intervals. A model for this retardation effect is useful in developing decision support tools, as well as in optimizing (perhaps minimizing) the use of this growth retardant. Such a model, as developed and described earlier, simulates the effect of a foliar spray application of daminozide at various concentrations on various days during the production cycle. The objective of this work was to validate this model for various varieties of chrysanthemum. Using the model to simulate the effect of one application of daminozide resulted in predicted plant heights very close to the observed heights for most of the varieties tested. Of four methods used to implement the multiple-application effect, two resulted in very good simulation of the observed plant heights. In summary, the model was shown to be valid for all the varieties of chrysanthemum tested.
George F. Antonious, Matthew E. Byers, John C. Snyder, and Douglas L. Dahlman
The development and deployment of crop varieties that resist or tolerate insect attack is one tactic of pest management that can eliminate one or more spray applications per season, a significant savings to the grower. Seven tomato, Lycopersicon esculentum Mill. cultivars (Marmand, Edkawy, VF-145, GS-27, Pakmore-B, Flordade, and UCX) were evaluated under greenhouse conditions for differences in mortality and feeding behavior (leaf-area ingested) of the 4th instar larvae of Spodoptera littoralis (Boisd) and the Colorado potato beetle, Leptinotarsa decemlineata (Say). The most resistant cultivars to S. littoralis during two summer seasons, 1990 and 1991, were Edkawy and UCX (37% mortality) and VF-145 (33% mortality). Mortality was least (20%) on the F1 hybrid GS-27, indicating that GS-27 was the most favorable cultivar for S. littoralis. L. decemlineata larvae reared on excised tomato leaflets of the same varieties indicated similar trends. Factors responsible for greater resistance of Edkawy and UCX to S. littoralis and L. decemlineata are under investigation.
Entin Daningsih, Laurie Hodges, and James R. Brandle
Field experiments were conducted in 1991, 1992, and 1993 to evaluate the effects of antitranspirant (Folicote, Aquatrol Inc.) and polyacrylamide gel (Supersorb, Aquatrol Inc.) on early growth of muskmelon. A RCBD with split plot arrangement was used with sheltered and exposed areas as the main treatments and seven combinations of antitranspirant spray and gel dip applications as subtreatments. Two greenhouse experiments were also conducted to simulate field research. A RCBD with seven treatments described as subtreatments in the field research was used in the greenhouse studies. Based on destructive harvests in the field, treatments and subtreatments did not affect dry weight or leaf area index. Specific contrasts, how ever, showed that gel application significantly increased dry weight and leaf area index whereas the spray application tended to reduce these factors during the first three weeks after transplanting. Significant differences between dip and spray subtreatments disappeared by five weeks after transplanting. In both greenhouse experiments, gel dip application increased dry weight and leaf area index of muskmelon at all observations from 2 weeks to five weeks after transplanting. We conclude that gel application generally will provide more benefit during early muskmelon growth compared to the use of antitranspirant spray.
Culentro, an umbelliferous aromatic saponin-containing biennial herb native of Central America and the West Indies is a major ingredient of many West Indian and Latin American dishes. Although closely related to the Asian culinary herb - cilantro or coriander, culantro is mainly prized for its green serrate spatulate-shaped leaves the main source of its oil. Like many other umbelliferoids under high temperatures and long summer days of the tropics culantro produces large umbel inflorescences and seedheads which are labor-intensive to remove, retard leaf growth and hence decrease the market value of the plant. Preliminary studies using ProGibb sprays from 50 to 200 ppm to 3- to 4-month old culantro plants grown under 53% shade showed increased leaf growth and chlorophyll content response to increasing levels of ProGibb. Maximum leaf length, fresh leaf weight, chlorophyll content and decreased flower growth were obtained at 100 ppm spray application. Treated plants remained in a vegetative phase for almost two years when vegetative side shoots were established. Postharvest observations showed no apparent decrease in shelf life nor loss of characteristic leaf aroma in leaves harvested from GA-treated plants. Inflorescences from sprayed plants were highly reduced in size, had leaf-like appearance and produced characteristic culantro aroma Indicating that they may also be utilized in culanto cuisines.
John E. Erwin and Gerald Pierson
Lypcopersicum esculentum cv `Money Maker' seeds were germinated at constant 20C. Three days after germination seedlings were randomly divided into 3 groups and placed into 3 growth chambers maintained at 23/17, 20/20, or 17/23C (day/night temperature) (DT/NT). Irradiance and photoperiod were maintained at 250 μmol s-1 m-2 and 12 hrs, respectively. At the 2 leaf stage, plants in each chamber were divided into 3 groups of 3 plants each to receive a growth regulator treatment. Growth regulator treatments consisted of spray applications of either ancymidol (52ppm), GA3 (12ppm), or water applied every 3 days for 21 days. Measurements were taken on internode length and chlorophyll content after 21 days. Internode length increased as the difference (DIF) between DT and NT increased (DT-NT). Exogenous applications of GA3 overcame inhibition of stem elongation resulting from a -DIF environment. Application of ancymidol did not significantly decrease stem elongation in a -DIF environment. Temperature regime had a significant impact on chlorophyll content per mg dry weight. In contrast, growth regulator applications had a significant impact on chlorophyll content cm-2. There was no significant impact of either temperature regime or growth regulator treatment on the chlorophyll a/b ratio.
Holly L. Scoggins* and Joyce G. Latimer
Increasing fertilizer levels may reduce production time but can lead to excessive growth of herbaceous perennials, requiring the application of plant growth regulators (PGRs). This study investigated the effects of ascending fertilizer rates in conjunction with two rates of uniconazole and a control. Rooted liners of Artemisia arborescens L. `Powis Castle', Artemisia vulgaris L. `Oriental Limelight, Astilbe chinensis (Maxim.) Franch. `Pumila', Filipendula rubra (Hill) Robinson `Venusta' and Perovskia atriplicifolia Benth. were potted with controlled-release fertilizer (15N-3.9P-10K) incorporated at 2.4, 4.72, and 7.11 kg·m-3. A single foliar spray application of uniconazole was applied two weeks after transplanting at a volume of 210 mL·m-3 and two rates from 15 to 60 mg·L-1 plus a control (species-dependent). Plant height and width were measured at 2,4,6, and 8 weeks after treatment (WAT). No interactions between fertilizer rate and uniconazole were observed. Main effects varied by species. The application of uniconazole controlled height and width of Artemisia `Oriental Limelight' and Astilbe for the duration of the experiment. Height, width, and dry weight of Artemisia `Oriental Limelight' increased with ascending fertilizer rates while Astilbe was not affected. Growth of Filipendula and Artemisia `Powis Castle' was unresponsive to uniconazole, though dry weight was reduced for both at the lowest fertilizer rate. Uniconazole provided height control of Perovskia, but the effect did not persist beyond 6 WAT. Ascending fertilizer rates increased Perovskia dry weight but not height.
Bruce W. Wood, Rufus Chaney, and Mark Crawford
The existence of nickel (Ni) deficiency in certain horticultural crops merits development of fertilizer products suitable for specific niche uses and for correcting or preventing deficiency problems before marketability and yields are affected. The efficacy of satisfying plant nutritional needs for Ni using biomass of Ni hyperaccumulator species was assessed. Aqueous extraction of Alyssum murale (Waldst. & Kit.) biomass yielded a Ni-enriched extract that, upon spray application, corrects and prevents Ni deficiency in pecan [Carya illinoinensis (Wangenh.) K. Koch]. The Ni-Alyssum biomass extract was as effective at correcting or preventing Ni deficiency as was a commercial Ni-sulfate salt. Foliar treatment of pecan with either source at ≥10 mg·L–1 Ni, regardless of source, prevented deficiency symptoms whereas treatment at less than 10 mg·L–1 Ni was only partially effective. Autumn application of Ni to foliage at 100 mg·L–1 Ni during leaf senescence resulted in enough remobilized Ni to prevent expression of morphologically based Ni deficiency symptoms the following spring. The study demonstrates that micronutrient deficiencies are potentially correctable using extracts of metal-accumulating plants.