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Wenhao Dai, Victoria Magnusson, and Chris Johnson

carbenicillin plus 15 mg·L −1 kanamycin (CCK 15 )] (antibiotics were added into the autoclaved medium when the medium temperature cooled down to ≈50 °C) for regeneration under shoot culture conditions previously mentioned. Calluses developed from leaf tissues

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Seong Min Woo and Hazel Y. Wetzstein

). Materials and Methods Plant material. Axenically grown shoot cultures of E. racemosa were the source of leaf tissues for culture induction studies. Proliferating cultures were previously established as described by Woo and Wetzstein (2008

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Jeffrey Adelberg, Maria Delgado, and Jeffrey Tomkins

water content of Cymbidium protocorm-like body and growth. In: L.F.M. Marcelis (ed.). Crop models in protected cultivation Acta Hort. 456 61 66 Kane, M. 2005 Shoot culture procedures 145 157 Trigiano R

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Wei-Ting Tsai and Chien-Young Chu

Eucalytpus axillary shoot cultures grown in liquid medium Plant Cell Tissue Organ Cult. 71 245 252 Wong, M.Y. Chu, C.Y. 1995 Effect of medium phase on the growth of rose explants in vitro J. Agr. For. 44 71 77

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Hisayo Yamane, Megumi Ichiki, Ryutaro Tao, Tomoya Esumi, Keizo Yonemori, Takeshi Niikawa, and Hino Motosugi

( Sugiura et al., 1986 ). The explants were placed in the initial shoot culture medium and subcultured at ≈3-week intervals. Murashige and Skoog medium (MS) containing 5 μ m zeatin was used as the basal medium. After 2 years of repeated subculturing, the

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Adam Dale, Becky R. Hughes, and Danielle Donnelly

10 subcultures. Shoots are indexed for the presence of bacteria and fungi on the first or second subculture. Shoot cultures that test negative for bacteria and fungi are subcultured onto rooting medium, and the rooted plantlets are transferred to the

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Hua Q. Zhao, Qing H. He, Li L. Song, Mei F. Hou, and Zhi G. Zhang

and explant types influence in vitro plant regeneration of Leopard Orchid ( Ansellia africana Lindl.) Plant Cell Tiss. Org. Cult. 107 123 129 Vinoth, A. Ravindhran, R. 2015 Reduced hyperhydricity in watermelon shoot cultures using silver ions In Vitro

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Rochelle R. Beasley and Paula M. Pijut

every 4 weeks for shoot culture establishment and continuous micropropagation through nodal sectioning. Rooting of adventitious shoots. Elongated microshoots (2 to 3 cm) were cultured in woody plant medium ( Lloyd and McCown, 1980 ) (WPM; L154

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Danny L. Barney, Omar A. Lopez, and Elizabeth King

additional research on media formulations. Our approach was to evaluate modifications of MS and WPM media, focusing on mineral nutrient concentrations. Materials and methods Establishment and maintenance of in vitro shoot cultures. Seeds from the three

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Jericó J. Bello-Bello, Lourdes G. Iglesias-Andreu, Susana A. Avilés-Viñas, Eunice Gómez-Uc, Adriana Canto-Flick, and Nancy Santana-Buzzy

.) HortScience 46 1666 1671 Sreedhar, R.V. Venkatachalam, L. Bhagyalakshmi, N. 2007 Genetic fidelity of long-term micropropagated shoot cultures of vanilla ( Vanilla planifolia Andrews) as assessed by molecular markers Biotechnol. J. 2 1007 1013 Stroud, H. Ding