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harvested at commercial maturity (135 d after full bloom) from a commercial plantation located in Fenghua, Zhejiang Province, China, and immediately transported to the laboratory. Treatments Fruit were precooled to 10 °C and manually selected for uniform

production and higher input costs for growers. Using precooling or forced air cooling along with commonly used ice packs could reduce and maintain box temperatures during storage and mitigate disease and leaf yellowing. Along with previous works regarding

enzymatic activity, accumulation of proteins and softening of persimmon ( Diospyros kaki Thunb.) flesh as a function of pre-cooling acclimatization Sci. Hort. 127 242 248 Duan, X. Liu, T. Zhang, D. Su, X. Lin, H. Jiang, Y. 2011 Effect of pure oxygen

the terminal whorl was cut into thin strips 2–3 mm wide along the length of the leaf. Each sample was weighed, flash frozen with liquid N and stored in precooled plastic tubes at −80 °C. Samples were lyophilized. Cytokinins were extracted and purified

(1996) . Polyamine analysis. Sharma and Rajam (1995) developed an improved method for quantifying putrescine, Spd and Spm. Using their methods, 0.5 g plant materials were added to 1.6 mL of 5% precooled perchlorate, ground in an ice bath, and

fruit were packaged in four 2-lb clamshells of 75 cherries each. Packed clamshells were fitted with a paper liner to absorb moisture, and were precooled to 0.1 °C before storing in fresh LOK Cherry Modified Atmosphere Packaging (MAP) bags (Shields Bag

pigments). The mortars, pestles, and reagents were precooled to 4 °C; all operations were conducted on ice and the extraction process was conducted in the dark. The extraction solution was subjected to ultrasonication (KQ-300E; Kunshan, Jiangsu Province

a 4 °C pre-cooled solution containing sodium carbonate (30 g·L –1 ), formaldehyde (0.35 mL·L –1 ), and sodium thiosulfate (0.05 g·L –1 ). The gel development was stopped by incubating in 10% acetic acid for 1 min. Gels were rinsed for 5 min and dried

under darkness after they were vortexed for 1 min twice; then, they were centrifuged at 13,000 g n for 5 min at 4 °C. The supernatants were collected in new 1.7-mL pre-cooled Eppendorf tubes and serial dilutions (up to three times) in a final volume of

cut flowers and foliage. Precooling is a very important step for achieving low-temperature storage and transportation. The optimal temperature is usually 2 to 3 °C, except for species such as celosia, oriental lilies, and zinnias, which should be kept