complete plantlets. The process of cell dedifferentiation can be seen by histological studies and determine regeneration pathways, and also confirms the presence of meristematic structures, which when divided by mitosis give rise to new structures
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Carlos Alberto Lecona-Guzmán, Sheila Reyes-Zambrano, Felipe Alonso Barredo-Pool, Miguel Abud-Archila, Joaquín Adolfo Montes-Molina, Reiner Rincón-Rosales, and Federico Antonio Gutierrez-Miceli
Hisanao Suzue, Munetaka Hosokawa, and Susumu Yazawa
compressed air method took one-eighth of this time ( Table 1 ). Table 1. Exposure time needed for removing all leaf primordia and exposing shoot apical meristems in different species. The results of the histological analysis showed no injured
Brent L. Black and Mark K. Ehlenfeldt
early as late July and that bud differentiation was asynchronous, progressing acropetally. Based on these histologic data, Retamales et al. (2000) applied GA 3 from 7 to 13 weeks after full bloom (AFB) to correspond to the meristematic transition
Yung-I. Lee, Chia-Fu Lu, Mei-Chu Chung, Edward C. Yeung, and Nean Lee
composed of seeds from those five capsules. Seeds were removed carefully from the placenta and then dried at 70 °C for 48 h. The water content was calculated as follows: Histologic preparations. Developing seeds were collected and fixed in a
Fengfeng Du, Xiaojing Liu, Yajun Chang, Naiwei Li, Yuesheng Ding, and Dongrui Yao
hybrids Timber Press London, UK Zhang, D.S. Chen, Q. Liu, Q.Q. Liu, F.L. Cui, L.J. Shao, W. Wu, S.H. Xu, J. Tian, D.K. 2019 Histological and cytological characterization of anther and appendage development in Asian lotus ( Nelumbo
Zhenghui Tang, Honghui Lin, Lei Shi, and Weilun Chen
rooting medium. The rooting criterion was the presence of at least one visible whitish, polar, cylindrical structure ≈2 mm long ( Corrêa et al., 2005 ). Histological examination. Histological analysis was made with light microscopy to study the
Gilles Galopin, Sandrine Codarin, Jean-Daniel Viemont, and Philippe Morel
Hitachi 4200 (Elexience, France) field emission instrument ( Défarge et al., 1999 ). For histological examination, the meristems were fixed in a 4% glutaraldehyde solution for 2 h. They were then dehydrated in an ethanol series (50%, 70%, 95%, and 100
Ying Chen, Xinlu Chen, Fei Hu, Hua Yang, Li Yue, Robert N. Trigiano, and Zong-Ming (Max) Cheng
treatment had three replicates per replication with 16 leaf segments. Histology light microscopy. For histology, samples were fixed in formalin, acetic acid, alcohol solution (FAA) containing 70% ethanol, 5% glacial acetic acid, and 5% formaldehyde at room
Carmen Valero Aracama, Michael E. Kane, Sandra B. Wilson, and Nancy L. Philman
measured at the substrate level. This experiment was replicated once in time and plants were transferred ex vitro between 2 Aug. and 13 Sept. 2003. Anatomical comparisons. Leaf histological cross-sections were made from EK 11-1 and EK 16
Nguyen Phuc Huy, Vu Quoc Luan, Le Kim Cuong, Nguyen Ba Nam, Hoang Thanh Tung, Vu Thi Hien, Dung Tien Le, Kee Yoeup Paek, and Duong Tan Nhut
. Histological study. Samples were fixed in Formalin acetic acid alcohol (FAA; formaline, acetic acid, and 70% ethanol as 5:5:90), dehydrated with Deshidratante histológico (Biopur SRL, Rosario, Argentina), embedded in paraffin wax (Paraplast Plus ® ; Sigma