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Carlos Alberto Lecona-Guzmán, Sheila Reyes-Zambrano, Felipe Alonso Barredo-Pool, Miguel Abud-Archila, Joaquín Adolfo Montes-Molina, Reiner Rincón-Rosales, and Federico Antonio Gutierrez-Miceli

complete plantlets. The process of cell dedifferentiation can be seen by histological studies and determine regeneration pathways, and also confirms the presence of meristematic structures, which when divided by mitosis give rise to new structures

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Hisanao Suzue, Munetaka Hosokawa, and Susumu Yazawa

compressed air method took one-eighth of this time ( Table 1 ). Table 1. Exposure time needed for removing all leaf primordia and exposing shoot apical meristems in different species. The results of the histological analysis showed no injured

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Brent L. Black and Mark K. Ehlenfeldt

early as late July and that bud differentiation was asynchronous, progressing acropetally. Based on these histologic data, Retamales et al. (2000) applied GA 3 from 7 to 13 weeks after full bloom (AFB) to correspond to the meristematic transition

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Yung-I. Lee, Chia-Fu Lu, Mei-Chu Chung, Edward C. Yeung, and Nean Lee

composed of seeds from those five capsules. Seeds were removed carefully from the placenta and then dried at 70 °C for 48 h. The water content was calculated as follows: Histologic preparations. Developing seeds were collected and fixed in a

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Fengfeng Du, Xiaojing Liu, Yajun Chang, Naiwei Li, Yuesheng Ding, and Dongrui Yao

hybrids Timber Press London, UK Zhang, D.S. Chen, Q. Liu, Q.Q. Liu, F.L. Cui, L.J. Shao, W. Wu, S.H. Xu, J. Tian, D.K. 2019 Histological and cytological characterization of anther and appendage development in Asian lotus ( Nelumbo

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Zhenghui Tang, Honghui Lin, Lei Shi, and Weilun Chen

rooting medium. The rooting criterion was the presence of at least one visible whitish, polar, cylindrical structure ≈2 mm long ( Corrêa et al., 2005 ). Histological examination. Histological analysis was made with light microscopy to study the

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Gilles Galopin, Sandrine Codarin, Jean-Daniel Viemont, and Philippe Morel

Hitachi 4200 (Elexience, France) field emission instrument ( Défarge et al., 1999 ). For histological examination, the meristems were fixed in a 4% glutaraldehyde solution for 2 h. They were then dehydrated in an ethanol series (50%, 70%, 95%, and 100

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Ying Chen, Xinlu Chen, Fei Hu, Hua Yang, Li Yue, Robert N. Trigiano, and Zong-Ming (Max) Cheng

treatment had three replicates per replication with 16 leaf segments. Histology light microscopy. For histology, samples were fixed in formalin, acetic acid, alcohol solution (FAA) containing 70% ethanol, 5% glacial acetic acid, and 5% formaldehyde at room

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Carmen Valero Aracama, Michael E. Kane, Sandra B. Wilson, and Nancy L. Philman

measured at the substrate level. This experiment was replicated once in time and plants were transferred ex vitro between 2 Aug. and 13 Sept. 2003. Anatomical comparisons. Leaf histological cross-sections were made from EK 11-1 and EK 16

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Yuyu Wang, Faju Chen, Yubing Wang, Xiaoling Li, and Hongwei Liang

°C for 20 min. Histology. Embryogenic tissues were collected during embryonic development of somatic embryos and were fixed in FAA solution (formalin:acetic acid:absolute ethanol:distilled water of 5:5:45:45, v/v/v/v) for 24 h at room temperature for