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Marianela Ramirez, Marek J. Krasowski, and Judy A. Loo

vitro contamination, increase rooting success, and acclimatize plantlets to greenhouse conditions; and Determining factors that contribute to the success or failure of grafts. Materials and Methods Study area. Material for this

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Ilse-Yazmín Arciniega-Carreón, Carmen Oliver-Salvador, María-Guadalupe Ramírez-Sotelo, and Carlos Edmundo Salas

acclimatization on day 25 ( Fig. 2C ) shows a robust increase in root induction accompanied by larger number of roots (4.25 ± 0.98). At 3 mg·mL −1 IBA, emergence of thin primary fasciculate roots declined by 40% compared with 2 mg·L −1 IBA ( Fig. 2D ; Table 4

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Aikaterini N. Martini and Maria Papafotiou

contribution of NaCl to better proliferation and rooting. Finally, various peat and perlite mixtures were examined for ex vitro acclimatization and establishment to develop an efficient micropropagation protocol for commercial use. Materials and Methods Effect

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Nittaya Chookoh, Yi-Tien Chiu, Chen Chang, Wei-Hsin Hu, and Ting-En Dai

. PLBs were converted into plantlets and then full plants, which were then acclimatized and allowed to flower. Materials and Methods Initiation of in vitro–grown donor plantlets. Tissue culture materials were obtained from the flower stalk nodes of the

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John E. Preece, Carl A. Huetteman, W. C. Ashby, and Paul L. Roth

During the research phase, a system was developed to clonally micropropagate silver maple. Explant performance was best on DKW medium with 10 nM thidiazuron, and explants commonly developed 1 7 shoots after three months and over 60 shoots that could be rooted after four months in vitro. Plants were rooted (>90%) and acclimatized under intermittent mist and transplanted to an outdoor nursery bed. However, results were different during the production phase when 90 clones were propagated. Shoot proliferation rates were lower, differences in clonal response and worker efficiency were apparent, mass rooting under mist was inconsistent and acclimatization problems arose. The mean rooting was 46% under mist because of uneven coverage. Only 56% of rooted plantlets acclimatized which resulted in an overall efficiency of 26%. Partial solutions included root initiation in vitro, and use of fog for acclimatization.

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Muhammad Irshad, Hafiz Muhammad Rizwan, Biswojit Debnath, Muhammad Anwar, Min Li, Shuang Liu, Bizhu He, and Dongliang Qiu

and acclimatization of plantlets. Elongated shoots (2.0–5.0 cm in length) were transferred to a rooting medium consisting half strength of MS salts, 200 mg·L −1 AC, and varying concentrations (0.5, 1, 1.5, 2, and 2.5 mg·L −1 ) of either indole-3

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Li Xu, Suzhen Huang, Yulin Han, and Haiyan Yuan

and plant height were evaluated 5 weeks after the culture. Acclimatization. Plantlets with well-developed roots and leaves were washed with running tap water to remove the culture media and transferred to plastic pots (30 cm × 30 cm) containing a

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Eucario Mancilla-Álvarez, Marco A. Ramírez-Mosqueda, Samantha Arano-Avalos, Rosalía Núñez-Pastrana, and Jericó J. Bello-Bello

. Acclimatization. Shoots measuring 5 to 7 cm in length and showing optimal root development were rinsed with tap water. Subsequently, shoots were planted in sterile peatmoss + agrolite (1:1 v/v) using 72-well trays (5 × 5 × 8 cm). Plantlets were kept under

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Ying Chen, Xinlu Chen, Fei Hu, Hua Yang, Li Yue, Robert N. Trigiano, and Zong-Ming (Max) Cheng

were recorded after 10 and 20 d in culture. Acclimatization. Plantlets ≈3 to 4 cm high with healthy roots were gently washed in tap water to remove agar residue and transplanted to wide pots (10 × 8.5 cm) containing PRO-Mix soil (Premier Horticulture

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Akira Sugiura, Yoshiko Matsuda-Habu, Mei Gao, Tomoya Esumi, and Ryutaro Tao

× 75 mm × 100 mm) containing vermiculite and acclimatized for ≈4 more weeks at 25 °C under a 14-h light/10-h dark photoperiod before potting. Statistical analysis. We calculated 95% confidence intervals of the globular embryo development, embryo