, pH 2.4, flow rate: 1.0 mL·min –1 , column temperature: 30 °C, detection wavelength 210 nm. Each sample was injected three times, and the corresponding organic acid or sugar content was calculated using the obtained standard curve. The average value
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Xiaoli Zhang, Qiang Liu, Shengyang Niu, Chonghuai Liu, Xiucai Fan, Ying Zhang, Lei Sun, and Jianfu Jiang
Nan Jia, Yonggang Yin, Minmin Li, Bin Han, Yan Sun, Changjiang Liu, Kunqi Tian, Shiyuan Liu, Yingjie Wang, and Zijuan Guo
phase of acids was KH 2 PO 4 (18 m m , pH 2.1) and flowed at a rate of 0.8 mL⋅min −1 . Sugar and acid amounts were determined using peak areas with external standards (fructose, glucose, sucrose, tartaric acid, malic acid, and citric acid) and expressed
Shu Hsien Hung, Chun Chi Wang, Sergei Veselinov Ivanov, Vera Alexieva, and Chih Wen Yu
followed the procedure of Anderson et al. (1992) . Mung bean leaves (1 g fresh weight) were homogenized in 3 mL of ice-cold acidic extraction buffer [6% (w/v) meta-phosphoric acid (pH 2.8), containing 1 m m EDTA], using a Polytron homogenizer (model PT
Cristián Vela-Hinojosa, Héctor B. Escalona-Buendía, José A. Mendoza-Espinoza, Juan M. Villa-Hernández, Ricardo Lobato-Ortíz, Juan E. Rodríguez-Pérez, and Laura J. Pérez-Flores
described by Nour et al. (2010) . Chromatographic separation was performed with an HPLC system (1260 Series; Agilent Technologies, Santa Clara, CA), using 50 m m potassium dihydrogen orthophosphate buffer, pH 2.8, as a mobile phase (flow rate of 0.7 mL
Renee T. Threlfall, Olivia S. Hines, John R. Clark, Luke R. Howard, Cindi R. Brownmiller, Daniela M. Segantini, and Lydia J.R. Lawless
thawed, placed in cheesecloth, and squeezed to extract the juice from the berries. Titratable acidity and pH were measured by an 877 Titrino Plus (Metrohm AG, Herisau, Switzerland) standardized to pH 2.0, 4.0, 7.0, and 10.0 buffers. Titratable acidity was
Yanina Perez Cayo, Steven Sargent, Cecilia do Nascimento Nunes, and Vance Whitaker
monobasic (KH 2 PO 4 , 0.5%, w/v) at pH 2.5 with metaphosphoric acid (HPO 3 , 0.1%, w/v). The retention time of the AA peak was 2.5 min. After comparison of retention time with the AA standard, the peak was identified. The amount of total AA content in
Derek W. Barchenger, John R. Clark, Renee T. Threlfall, Luke R. Howard, and Cindi R. Brownmiller
automated titrimeter and electrode standardized to pH 2.0, 4.0, 7.0, and 10.0 buffers. Titratable acidity was determined by titrating 0.1 N sodium hydroxide to 6 g of juice diluted with 50 mL of deionized, degassed water to an endpoint of pH 8.2, with
Molly Felts, Renee T. Threlfall, and Margaret L. Worthington
-H refill cartridge (30 × 4.5 mm) was used for a guard column. Columns were maintained at 65 °C by a temperature control unit. Mobile phase consisted of a pH 2.28 solution of sulfuric acid and water with a resistivity of 18 M obtained from a Millipore Milli
Huan Zhang, Lisa Wasko DeVetter, Edward Scheenstra, and Carol Miles
temperature ( WSU AgWeatherNet, 2019 ). During the growing season, the average RH is 80%, while total precipitation is 39 mm per month. The soil is Skagit silt loam, a fine-silty mixed nonacid mesic Typic Fluvaquents, with 6.5 pH, 2.7% organic matter, and is
Kellie J. Walters, Bridget K. Behe, Christopher J. Currey, and Roberto G. Lopez
with the producers reporting research on nutrient solution formulations (mean = 3.1 ± 1.0) and nutrient solution pH (2.8 ± 1.0) being slightly to very beneficial ( Fig. 6 ). Additionally, with the majority of producers surveyed monitoring and