Experiments were conducted to determine if the seedling hypocotyl elongation and petal abscission assays could be used to identify differences in ethylene sensitivity among seedling geranium (Pelargonium ×hortorum) cultivars. When seedlings of six geranium cultivars were germinated and grown in the dark in the presence of the ethylene biosynthetic precursor 1-aminocyclopropane-1-carboxylic acid (ACC) at various concentrations, they exhibited the triple response (measured as reduced hypocotyl length). While seedlings from all six cultivars were sensitive to ACC, `Scarlet Elite' seedlings were most sensitive, and `Multibloom Lavender', `Elite White' and `Ringo 2000 Salmon' seedlings were the least sensitive when germinated and grown on 20 mm [2022 mg·L-1 (ppm)] ACC. Florets representing three developmental stages of each of the six cultivars were exposed to 1 μL·L-1 of exogenous ethylene for 0, 30, or 60 min to determine if differences in cultivar sensitivity could be determined for petal abscission. Of the six cultivars tested, `Ringo 2000 Salmon', `Multibloom Lavender' and `Elite White' were the least ethylene sensitive. Florets were also self-pollinated to test for cultivar differences in ethylene synthesis and subsequent petal abscission. Ethylene production and petal abscission were both promoted in self-pollinated florets compared to nonpollinated florets. `Ringo 2000 Salmon', `Multibloom Lavender' and `Elite White' florets produced similar amounts of ethylene as all other cultivars, but abscised fewer petals after pollination. Our results indicate that the seedling hypocotyls elongation assay may be used to identify geranium cultivars with reduced sensitivity to ethylene. The data also suggest that genetic variability exists among geraniums for both ethylene sensitivity and biosynthesis.
David G. Clark, Christopher Dervinis, James E. Barrett, and Terril A. Nell
Jane Whittaker, Terril A. Nell, James E. Barrett, and Thomas J. Sheehan
The effect of postharvest dips on the longevity of Anthurium andraenum cultivar Nitta and Alpinia purpurata was evaluated. The inflorescences were dipped in a 200 ppm benzyladenine (BA) solution, an antitranspirant, or water for 10 minutes. After dipping, anthuriums were placed directly in water and gingers were placed in either water or a 2% sucrose solution and placed in interior conditions (10 μmol m-2s-1 for 12 hr/day, 21±2C). Ginger longevity was increased by 10 days or more by the sucrose solution. The greatest longevity of gingers was obtained when dipped in either BA or the antitranspirant and held in the sucrose solution. Anthurium longevity increased 10 days when dipped in BA, while the other treatments had little effect.
David G. Clark, Chris Dervinis, James E. Barrett, Harry Klee, and Michelle Jones
Cytokinins have been shown to delay the onset of leaf senescence. The focus of this project was to produce transgenic petunia (Petunia ×hybrida) plants that over-produced endogenous cytokinins in a senescence specific manner. This was achieved by transforming plants with the IPT (isopentenyl transferase) gene driven by the senescence-associated transcriptional promoter, PSAG12. Two independent transgenic events produced T1 and T2 generation seedling lines that demonstrated the desired nonsenescent phenotype in progeny trials. These lines were used to evaluate the horticultural performance of PSAG12-IPT petunia plants in terms of delayed senescence, rooting of vegetative cuttings, lateral branch growth, flower number, floral timing, and fruit set. Although both lines displayed a delayed senescence phenotype the two PSAG12-IPT transgenic lines differed from each other in regard to other horticultural traits. In addition to delayed leaf senescence, line I-1-7 also demonstrated a decrease in adventitious rooting and an increased number of branches during plant production. Line I-3-18 also demonstrated a delayed leaf senescence phenotype; however, plants of this line were not greatly altered in any other horticultural performance traits in comparison to wild-type `V26'. IPT transcript was detected in young fully expanded leaves of both lines, although mRNA levels were higher in I-1-7 plants. A greater than 50-fold increase in IPT transcript abundance was detected in leaves of transgenic plants following drought stress. These results demonstrate that it is possible to use PSAG12-IPT to produce transgenic plants with delayed leaf senescence but differences in plant morphology between transgenic lines exist, which may alter horticultural performance characteristics.
Jeff B. Million, James E. Barrett, Terril A. Nell, and David G. Clark
Dendranthema×grandiflorum (Ramat.) were grown in either a peat-based or pine bark—based medium and drenched with growth retardants at a range of concentrations to generate dose : response curves. The effect of ancymidol, paclobutrazol, and uniconazole on stem elongation was less in the pine bark—based than in the peat-based medium. Generally, the concentrations required to achieve the same response were 3- to 4-fold as high in the pine bark—based medium as in the peat-based medium. However, chlormequat was slightly more active in the pine bark—based medium than in the peat-based medium. Chemical names used: α-cyclopropyl-α—(4-methoxyphenyl)-5-pyrimidinemethanol (ancymidol); (±)-(R*,R*)-β-[(4-chlorophenyl)methyl]-α-(1,1-di methyl)-1H-1,2,4-triazole-1-ethanol (paclobutrazol); (E)-(RS)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent -l-en-3-ol (uniconazole); 2-chloroethyltrimethylammonium chloride (chlormequat).
G.H. Pemberton, Terril A. Nell, Ria T. Leonard, A.A. De Hertogh, Lena Gallitano, and James E. Barrett
Forced `Bumalda' and `Etna' Astilbe were evaluated for postproduction quality and longevity. Plants were sleeved, boxed and held at 9±2C for 3 days to simulate shipping at the following stages of floral development: tight bud (TB), 1-3 florets open, 25% florets open, 50% florets open, and 75% florets open. They were then placed at 21C and 14 μmol·m-2·s-1 (12h daylength) until flower senescence. Percent of inflorescences flowering increased from 34% at TB stage to 94% when shipped with 25 % of the florets open. `Etna' longevity increased from 3 days at TB stage to 12 days at 25% open stage. Optimum quality and longevity occurred when ≥ 25% of the florets were opened at shipping.
In a second experiment, `Bumalda' and `Etna' Astilbe were held at 18, 21 and 24C at irradiance levels of 7 or 14 μmol·m-2·s-1 when 25% of the florets were open. At 18C, longevity increased under 14 μmol·m-2·s-1 from 14 to 17 days. At 24C, longevity was only 10 days for both irradiance levels.