Male-sterile, male-fertile, exserted stigma, and exserted stigma with positional sterile (ps) genotypes of tomato (Lycopersicon esculentum Mill.) were pollinated at daily intervals from one day before to 2 days after anthesis (A-1, A0, A+1, A+2, respectively) in 2 field experiments and one greenhouse experiment. A male-sterile with gene ms-10 35 had greater seeds per pollination than other genotypes in all 3 experiments. Seeds per pollination produced in the greenhouse was greater than that of a similar field experiment at all pollination stages except A-1, where seed production was similar. Seeds per pollination was less at A-1 than at later pollination stages. This was due to significant differences in seeds per fruit in all experiments and percentage of fruit set in 2 of 3 experiments. There was no selfing in the 2 male-sterile genotypes in any experiment and no selfing for exserted stigma with ps in the field experiments. There was about 2% selfing with ps at A+2 in the greenhouse. Selfing with stigma exsertion alone ranged from 2% to 22% in the field and from less than 1% to about 5% in the greenhouse. The relationship of selfing with pollination stage was not clear for the exserted stigma genotype.