Cool-season turfgrasses may experience heat stress during summer. Hybrid bluegrasses (HBGs), crosses between kentucky bluegrass [KBG (Poa pratensis L.)] and native texas bluegrass (Poa arachnifera Torr.), have improved heat tolerance but the mechanisms of heat tolerance are poorly understood. Our objectives were to quantitatively profile membrane lipid molecular species in three cool-season turfgrasses exposed to optimal (22/15 °C, 14/10 h light/dark) and supra-optimal temperatures (35/25 °C and 40/30 °C, 14/10 h light/dark). Grasses included a low heat-tolerant tall fescue [TF (Festuca arundinacea Schreb. ‘Dynasty’)], a mid-heat–tolerant KBG (‘Apollo’), and a heat-tolerant HBG (‘Thermal Blue’). At high temperature, glycolipid digalactosyldiacylglycerol (DGDG) in HBG was 12% and 16% greater than in KBG and TF, respectively, and the ratio DGDG to monogalactosyldiacylglycerol was 19% and 44% greater in HBG than in KBG and TF, respectively. Greater heat tolerance in HBG and KBG was associated with higher contents of phosphatidylethanolamine and phosphatidylglycerol, and with reduced overall unsaturation compared with TF. Overall, 20 lipid molecular species were present in greater amounts and another 20 species in lesser amounts in HBG and KBG than in TF. Results suggest 40 membrane lipid molecules are potential biomarkers for heat tolerance and that compositional changes in membrane lipids in response to heat contribute to differences in heat tolerance among cool-season grasses.
Kemin Su, Dale J. Bremer, Richard Jeannotte, Ruth Welti, and Celeste Yang
John W. Markham III, Dale J. Bremer, Cheryl R. Boyer, and Kenneth R. Schroeder
Heat stress is problematic to root growth in the production of containerized nursery plants. Container color may moderate effects of solar radiation on substrate temperatures. Studies were conducted near Manhattan, KS, to evaluate effects of container color on growth of roots and shoots in bush beans (Phaseolus vulgaris L.), red maple (Acer rubrum L.), and eastern redbud (Cercis canadensis L.). Four treatments among studies included containers colored flat and gloss white, silver, and black; a green container color treatment was added to the tree studies. Plants were grown in bark-based soil-less substrate and temperatures were measured at 5-cm depths in the south sides and centers. After 4 months, plant variables were measured. Roots were separated into three sections: core, north, and south. In the bean study, substrate temperatures at the south side of the container averaged lowest in flat and gloss white (≈36 °C) and greatest in black containers (50.3 °C). Root density at the south side was reduced in beans by 63% to 71% in black compared with flat and gloss white. In heat-sensitive maples, substrate temperatures at the south side of containers averaged up to 7.7 °C greater in black and green than in other treatments. Substrate temperatures in the center averaged 3.5 to 3.8 °C greater in black than in flat and gloss white, resulting in up to 2.5 times greater root density in flat and gloss white than in black containers. In heat-tolerant redbuds, the effects of container color on whole-plant growth were less evident. Data suggest that heat-sensitive plants benefit from being grown in white containers or painting outer surfaces of green and black containers white.
David O. Okeyo, Jack D. Fry, Dale J. Bremer, Ambika Chandra, A. Dennis Genovesi, and Milton C. Engelke
Zoysiagrass (Zoysia spp.) grown under shade on golf courses and in home lawns is slow to recover from damage and declines in quality over time. We evaluated stolon growth and tillering of ‘Meyer’ and Chinese Common (both Z. japonica Steud.); ‘Zorro’, ‘Diamond’, and ‘Cavalier’ [all Z. matrella L. (Merr.)]; ‘Emerald’ (Z. matrella × Z. pacifica Goudsw.); and six experimental progeny from ‘Emerald’ × Z. japonica and reciprocal crosses of Z. japonica × Z. matrella under silver maple (Acer saccharinum L.) shade and in full sun in 2008 and 2009 in Manhattan, KS. A single 6-cm diameter plug was planted in the center of 1.2 m × 1.2-m plots, and data were collected weekly on the number of stolons, stolon elongation, and number of stolon branches. Tiller number was collected at the start and end of each study period, and biomass (excluding roots) was determined at the end of each season. Zoysiagrasses under an average of 76% tree shade exhibited reductions of 38% to 95% in stolon number; 9% to 70% in stolon length; 10% to 93% in stolon branching; and 56% to 98% in biomass. Seven of the 10 grasses exhibited a decline in tiller number in each experiment; none of the grasses differed from ‘Meyer’ in percentage change in tiller number under shade. ‘Emerald’, ‘Cavalier’, ‘Zorro’, and several progeny from crosses between ‘Emerald’ × Z. japonica or reciprocal crosses of Z. matrella × Z. japonica produced more, longer, or more highly branched stolons than ‘Meyer’, suggesting they may have improved recovery potential in shade.