We studied the genetic variability of some traditional tomato (Lycopersicon esculentum L. Mill.) cultivars of Spain, and established their relationships using both simple sequence repeats (SSR) and sequence related amplified polymorphism (SRAP) markers. These included cultivars from different locations of three main types, Muchamiel, De la pera, and Moruno. Additionally we tested two other local cultivars, `Valenciano' and `Flor de Baladre', plus a small sample of commercial cultivars and a few wild species. Both types of markers resolved the cultivars from different groups, but SSR failed to distinguish some of those classified under the same group. All the De la pera cultivars clustered together by genetic similarity with the SRAP markers. The other traditional cultivars, which are grown in a wider geographic range, formed a more diffuse group, which included the commercial cultivar Roma. The Mexican cultivar Zapotec, a breeding line, and the virus-resistant commercial hybrid `Anastasia' were the most distant of all the cultivars. The latter hybrid had higher similarity to the wild species due to introgressed segments from them carrying the resistance genes. Similar results were observed for SSR markers but with a lower level of resolution. This information would be useful to facilitate tomato germplasm conservation and management efforts.
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