Factors Affecting Seed Production in Transgenic Ethylene-insensitive Petunias

in Journal of the American Society for Horticultural Science
View More View Less
  • 1 Department of Environmental Horticulture, University of Florida, Gainesville, FL 32611-0670
  • | 2 Department of Horticultural Sciences, University of Florida, Gainesville, FL 32611-0690
  • | 3 Department of Environmental Horticulture, University of Florida, Gainesville, FL 32611-0670

Pollen viability, in-vivo pollen tube growth, fruit ripening, seed germination, seed weight, whole plant vigor, and natural flower senescence were investigated in homozygous and heterozygous transgenic ethylene-insensitive CaMV35S::etr1-1 petunias (Petunia ×hybrida `Mitchell Diploid'). Homozygous or heterozygous plants were used to determine any maternal and/or paternal effects of the CaMV35S::etr1-1 transgene. All experiments except for those used to determine natural flower senescence characteristics were conducted in both high and low temperature greenhouses to determine the effect of temperature stress on transgenic plants when compared to wild-type. Results indicated that ethylene-insensitive plants had a decrease in pollen viability, root dry mass, seed weight, and seed germination. Fruit ripening, seed germination, and seed weight were maternally regulated. In contrast, the CaMV35S::etr1-1 transgene is completely dominant in its effect on natural flower senescence.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 249 59 1
PDF Downloads 403 104 19