Gene linkage was investigated in 11 families using 18 genes in cucumber (Cucumis sativus L.). The genes studied were B (black spine), B-3 (Black spine-3), B-4 (Black spine-4), bi (bitterfree cotyledons), Bt (bitter fruit), Bt-2 (bitter fruit-2), D (dull fruit skin), df (delayed flowering), de (determinate habit), F (female sex expression), gl (glabrous foliage), lh (long hypocotyl), ns (numerous spines), pm-h [powdery mildew (Sphaerotheca fuliginea Schlecht.:Fr.) resistance expressed on the hypocotyl], ss (small spines), Tu (tuberculate fruit), u (uniform immature fruit color), and w (white immature fruit color). A major objective of this study was to measure linkages of genes for fruit bitterness (Bt and Bt-2), and spine color (B-3 and B-4) relative to previously studied loci: B, bi, D, de, df, F, gl, lh, ns, pm-h, ss, Tu, u, and w. The F2 progeny of LJ 90430 × PI 173889 segregated 13 bitter fruit: 3 nonbitter fruit, indicating that different genes are controlling fruit bitterness in these lines. Bt-2 is proposed as the gene controlling bitterness of fruit in LJ 90430. It is a separate locus from Bt, that causes bitter fruit in PI 173889. Several new gene linkages were found: bi—Bt, (Bt-2)—de, D—(Bt-2), D—ns, gl—F, ss—(Bt-2), Tu—(Bt-2), and u—(Bt-2). The Bt gene appears to be linked to bi and may be located on linkage group I. Bt-2 appears to be linked with several genes that could connect linkage groups I and IV. Bt-2 was linked to u, Tu, D, and ss, that are all on linkage group IV. Bt-2 was also found to be linked loosely to de, that is on linkage group I. No linkages were found between B-3 and B-4 and the genes evaluated in this study. Weak linkages (>25 cM) between several gene combinations [(Bt-2)-de, de—ns, de—ss, de—Tu, de—u, ns—F, and ss—F] provided more evidence that linkage group I and IV may be linked. Due to the weak linkages, more information needs to be obtained using larger populations and more markers to confirm these findings.