Linkage Mapping in a Watermelon Population Segregating for Fusarium Wilt Resistance

in Journal of the American Society for Horticultural Science
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  • 1 Department of Horticulture, 101 Funchess Hall, Auburn University, Auburn, AL 36849-5408
  • | 2 U.S. Department of Agriculture Forest Service, Southern Institute for Forest Genetics, 23332 Highway 67, Saucier, MS 39574
  • | 3 Department of Horticulture, E142 Poole Center, Clemson University, Clemson, SC 29634-0375
  • | 4 U.S. Department of Agriculture, Agricultural Research Service, 1109 Experiment Street, Griffin, GA 30233

Isozyme, randomly amplified polymorphic DNA (RAPD), and simple sequence repeats (SSR) markers were used to generate a linkage map in an F2 and F3 watermelon [Citrullus lanatus (Thumb.) Matsum. & Nakai] population derived from a cross between the fusarium wilt (Fusarium oxysporum f. sp. niveum) susceptible `New Hampshire Midget' and resistant PI 296341-FR. A 112.9 cM RAPD-based map consisting of 26 markers spanning two linkage groups was generated with F2 data. With F3 data, a 139 cM RAPD-based map consisting of 13 markers covering five linkage groups was constructed. Isozyme and SSR markers were unlinked. About 40% to 48% of the RAPD markers were significantly skewed from expected Mendelian segregation ratios in both generations. Bulked segregant analysis and single-factor analysis of variance were employed to identify RAPD markers linked to fusarium wilt caused by races 1 and 2 of F. oxysporum f. sp. niveum. Current linkage estimates between the resistance trait and the marker loci were too large for effective use in a marker-assisted selection program.

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