Coexpression of Potato PVYo Coat Protein and cryV-Bt Genes in Potato

in Journal of the American Society for Horticultural Science
Authors:
Wenbin LiBiotechnology Division, Department of Plant Agriculture, Guelph University, Guelph, Ontario, Canada N1G 2W1

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Kelly A. ZarkaDepartment of Crop and Soil Sciences, Michigan State University, East Lansing, MI 48824

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David S. DouchesDepartment of Crop and Soil Sciences, Michigan State University, East Lansing, MI 48824

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Joseph J. CoombsDepartment of Crop and Soil Sciences, Michigan State University, East Lansing, MI 48824

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Walter L. PettDepartment of Entomology, Michigan State University, East Lansing, MI 48824

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Edward J. GrafiusDepartment of Entomology, Michigan State University, East Lansing, MI 48824

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The codon-modified cryV-Bt gene (cryV-Bt) from Bacillus thuringiensis subsp. kurstaki Berliner, which is specifically toxic to Lepidoptera and Coleoptera insects, and a potato virus Yo coat protein gene (PVYocp), in which the aphid transmission site was inactivated, were cotransformed into potato (Solanum tuberosum L.) `Spunta' via Agrobacterium tumefaciens Conn. We demonstrated the integration and expression of both genes by molecular analysis and bioassays. All cryV-Bt/PVYocp-transgenic lines were more resistant to potato tuber moth (Phthorimaea operculella Zeller) and PVYo infection than nontransgenic `Spunta'. Four cryV-Bt/PVYocp transgenic lines were equal in potato tuber moth mortality to a cryV-Bt transgenic line, but of these four only two lines were equivalent in PVYo titer levels to a PVYocp-transgenic line. We identified two transgenic lines, 6a-3 and 6a-5, which showed greater resistance to potato tuber moth and PVYo than the other cryV-Bt/PVYocp transgenic lines. This study indicated that multiple genes, conferring insect pest resistance and virus resistance, could be engineered into and expressed simultaneously in a potato cultivar.

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