Molecular Markers for the Scab Resistance (Vf) Region in Apple

in Journal of the American Society for Horticultural Science
Authors:
Minou HemmatCornell University, New York State Agricultural Experiment Station, Geneva, NY 14456

Search for other papers by Minou Hemmat in
ASHS
Google Scholar
PubMed
Close
,
Norman F. WeedenCornell University, New York State Agricultural Experiment Station, Geneva, NY 14456

Search for other papers by Norman F. Weeden in
ASHS
Google Scholar
PubMed
Close
,
Herb S. AldwinckleCornell University, New York State Agricultural Experiment Station, Geneva, NY 14456

Search for other papers by Herb S. Aldwinckle in
ASHS
Google Scholar
PubMed
Close
, and
Susan K. BrownCornell University, New York State Agricultural Experiment Station, Geneva, NY 14456

Search for other papers by Susan K. Brown in
ASHS
Google Scholar
PubMed
Close

Bulked segregant analysis was used to identify RAPD markers that display tight linkage to the Vf gene in apple (Malus sp.) that confers resistance to five races of apple scab [Venturia inaequalis (Cke.) Wint.]. We identified several new RAPD markers linked to Vf. The most tightly linked marker in the test population, S52500, was cloned and sequenced. A linkage map of the Vf region was developed using these markers, RAPD markers previously described by other laboratories, and the isozyme locus Pgm-1. An assay was developed for Vf by multiplexing the two markers closely flanking the Vf locus. This assay has a theoretical `escape' value (discarding a resistant plant) of 3% and an error rate (selection of a susceptible plant) of 0.02%.

  • Collapse
  • Expand