Molecular-marker-based Genetic Analysis of Tepary Bean-derived Common Bacterial Blight Resistance in Different Developmental Stages of Common Bean

in Journal of the American Society for Horticultural Science
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  • 1 Department of Horticulture, University of Wisconsin, Madison, WI 53706
  • | 2 Department of Horticulture, University of Nebraska, Lincoln, NE 68583
  • | 3 Department of Horticulture, University of Wisconsin, Madison, WI 53706
  • | 4 Arroyo Loro Experiment Station, San Juan de la Maguana, Dominican Republic
  • | 5 Department of Horticulture, University of Nebraska, Lincoln, NE 68583
  • | 6 Department of Agronomy, University of Wisconsin, Madison, WI 53706
  • | 7 Horticultural Sciences Department, University of Florida, Gainesville, FL 32611

Randomly amplified polymorphic DNA (RAPD) molecular markers were used to construct a partial genetic linkage map in a recombinant inbred population derived from the common bean (Phaseolus vulgaris L.) cross PC-50 × XAN-159 for studying the genetics of bacterial disease resistance in common bean. The linkage map spanned 426 cM and included 168 RAPD markers and 2 classical markers with 11 unassigned markers. The seventy recombinant inbred lines were evaluated for resistance to two strains of common bacterial blight [Xanthomonas campestris pv. phaseoli (Smith) Dye] (Xcp). Common bacterial blight (CBB) resistance was evaluated for Xcp strain EK-11 in later-developed trifoliolate leaves and for Xcp strains, DR-7 and EK-11, in first trifoliolate leaves, seeds, and pods. One to four quantitative trait loci (QTLs) accounted for 18% to 53% of the phenotypic variation for traits. Most significant effects for CBB resistance were associated with one chromosomal region on linkage group 5 and with two regions on linkage group 1, of the partial linkage map. The chromosomal region (a 13-cM interval) in linkage group 5 was significantly associated with resistance to Xcp strains DR-7 and EK-11 in leaves, pods, and seeds. The regions in linkage group 1 were also significantly associated with resistance to both Xcp strains in more than one plant organ. In addition, a seedcoat pattern gene (C) and a flower color gene (vlae) were mapped in linkage groups 1 and 5, respectively, of the partial linkage map. The V locus was found to be linked to a QTL with a major effect on CBB resistance.

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