Isozyme, Randomly Amplified Polymorphic DNA (RAPD), and Restriction Fragment-length Polymorphism (RFLP) Markers Used to Deduce a Putative Parent for the `Braeburn' Apple

in Journal of the American Society for Horticultural Science
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  • 1 The Horticulture and Food Research Institute of New Zealand, Batchelar Research Centre, Private Bag 11 030, Palmerston North, New Zealand
  • 2 The Horticulture and Food Research Institute of New Zealand, Havelock North Research Centre, Private Bag 1401, Havelock North, New Zealand

Information about a rare allele of phosphoglucomutase (PGM) that is shared by `Braeburn' and 16% of cultivars in the New Zealand Cultivar Collection was combined with historical information about cultivar distribution to select a set of 15 cultivars for a more detailed genetic analysis of their relatedness to the key New Zealand apple (Malus domestica Borkh.) `Braeburn'. DNA from all 16 cultivars was examined by RFLP analysis using 41 probe-enzyme combinations and also by RAPD analysis with 39 selected primers. The RFLP and RAPD data excluded a proposal that `Lady Hamilton' and `Braeburn' are genetically identical. All cultivars except `Lady Hamilton' were excluded as potential parents for `Braeburn' based on incompatible RFLP banding. Assessment of genetic distances between `Braeburn' and the other 15 cultivars from RFLP and RAPD data demonstrated that `Lady Hamilton' was more closely related to `Braeburn' than all others. We conclude that there is a high likelihood that `Lady Hamilton' is one of the parents of `Braeburn'.

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