Level of Heterozygosity and Mode of Inheritance of Variable Number of Tandem Repeat Loci in Avocado

in Journal of the American Society for Horticultural Science
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  • 1 Department of Fruit Trees, Genetics and Breeding, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
  • 2 Department of Genetics, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot 76100, Israel
  • 3 Department of Fruit Trees, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
  • 4 Department of Fruit Trees, Genetics and Breeding, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel

To estimate heterozygosity level in the avocado (Persea americana Mill.) genome, two types of variable number of tandem repeat (VNTR) markers were used. Multilocus DNA fingerprints (DFPs) were analyzed on avocado progeny resulting from either crosses or selfing of cultivars. In five crosses, heterozygosity was 100%, while in two self-pollinated families, heterozygosity was 90% and 94%. Single locus, simple sequence repeat (SSR) DNA markers were analyzed by typing 59 loci on five avocado cultivars. Average heterozygosity varied from 0.50 to 0.66, while gene diversity varied from 0.42 to 0.66. Heterozygosity varied from 38% to 70%. The percentage of fragments that exhibited Mendelian inheritance was 62.5% to 85% (P < 0.05) for the DFP fragments and 85% for the SSR alleles.

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