Randomly Amplified Polymorphic DNA Loci from a Walnut Backcross [(Juglans hindsii × J. regia) × J. regia]

in Journal of the American Society for Horticultural Science
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  • 1 Molecular Biology Research Facility, Laboratory for Molecular Biology (M/C 567), University of Illinois at Chicago, 900 South Ashland, Chicago, IL 60607
  • 2 Department of Pomology, University of California, Davis, CA 95616
  • 3 Department of Plant and Soil Sciences, University of Massachusetts, Amherst, MA 01003

Twenty-five random decamer primers were used to evaluate the level of polymorphism between Persian walnut and the Northern California black walnut. Sixty-six randomly amplified polymorphic DNA (RAPD) markers were identified using an interspecific walnut backcross population [(Juglans hindsii × J. regia) × J. regia]. Segregation data from these polymorphisms were joined to a previously published set of restriction fragment-length polymorphism (RFLP) marker data to expand the genetic map of walnut to 107 markers in 15 linkage groups.

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