RAPD Markers Flanking the Are Gene for Anthracnose Resistance in Common Bean

in Journal of the American Society for Horticultural Science
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  • 1 Department of Crop and Soil Sciences, Michigan State University, East Lansing MI, 48824

Incorporation of the dominant gene Are, of Middle American origin, into commercial cultivars of Phaseolus vulgaris L., has been the main disease control strategy of plant breeders to limit the potential damage of Colletotrichum lindemuthianum (Sacc. & Magnus.) Lams.-Scrib. A random amplified polymorphic DNA (RAPD) marker designated OQ41440, generated by a 5′-AGTGCGCTGA-3′ decamer primer, was found tightly linked in coupling with the Are gene. OQ41440 mapped at 2.0 ± 1.4 centimorgans (cM) from the Are allele in the Andean genetic background and at 5.5 ± 2.3 CM in the Middle American background. A second coupling phase RAPD marker B3551000, generated by the 5′-GTATGGGGCT 3′ primer mapped at 5.4 ± 2.3 cM from the Are allele in the Andean genetic background and at 7.7 ± 2.7 CM in the Middle American background. Based on a recombination distance of 7.0 ± 1.9 cM between the two markers, OQ41440 and B3551000 RAPDs appear to flank the Are gene. The bracketing molecular markers allowed tagging of the Are allele with a selection fidelity of 99%. Use of the OQ41440 and B3551000 RAPD markers for marker-based selection will afford the opportunity to retain the Are anthracnose resistance gene in bean germplasm, as other epistatic resistance genes are characterized, and incorporated into contemporary bean cultivars.

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