Genetic Similarity Among Brassica oleracea L. Genotypes as Measured by Restriction Fragment Length Polymorphisms

in Journal of the American Society for Horticultural Science
View More View Less
  • 1 Department of Horticulture, University of Wisconsin, Madison, WI 53706
  • | 2 Department of Biology, University of Utah, Salt Lake City, UT 84112
  • | 3 Harris-Moran Seed Company, 100 Breen Road, San Juan Bautista, CA 95045
  • | 4 Sun Seeds, 8850 59th Avenue, NE, Brooks, OR 97305

Genetic similarities were calculated among 89 Brassica oleracea L. genotypes, which included 62 broccolis (var. italica), 16 cauliflowers (var. botrytis), and 11 cabbages (var. capitata). These entries represented a wide range of commercially available germplasm, including open-pollinated cultivars, commercial hybrids, the inbred parents of several hybrid cultivars, and 27 entries that were provided as unknowns. Sixteen random genomic clones were used as probes in Southern hybridizations to detect restriction fragment length polymorphism (RFLP). From each of the random probes, an average of four polymorphic bands were classified as to their presence or absence for each genotype. The genetic similarity between ail pairs of genotypes was calculated. A multidimensional scaling (MDS) plot indicated that the broccoli, cauliflower, and cabbage groups were clustered with very little overlap. Within groups, genetic similarity corresponded to relationships based on available pedigree information. Comparison of banding patterns between hypothetical and actual hybrids was used to correctly identify the parents of several parent-hybrid combinations. The RFLP pattern of a hybrid and one of the parents (female) were used to predict the genotype and identity of the other parent (male).

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 88 24 0
PDF Downloads 141 56 12