Surface Chemical Differences Between Sweetpotato Lines with Varying Levels of Resistance to the Sweetpotato Weevil

in Journal of the American Society for Horticultural Science
Authors:
Ki-Cheol SonDepartment of Horticulture, University of Georgia, Athens, GA 30602

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Ray F. SeversonTobacco Quality and Safety Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Athens, GA 30613

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Richard F. ArrendaleTobacco Quality and Safety Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Athens, GA 30613

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Stanley J. KaysDepartment of Horticulture, University of Georgia, Athens, GA 30602

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Methodology was developed for the extraction of surface components of sweetpotato [Ipomoea batatas (L.) Lam.] storage roots. Surface components of storage roots were quantitatively extracted with methylene chloride using 8-minute ultrasonication. After removal of the solvent, the extract was treated with 3 Tri Sil-Z:1 trimethylsilylimidazol (v/v) to convert components with hydroxyl moieties to silyl ethers and then separated on a SE-54 fused silica capillary column. Distinctly different gas chromatography profiles were found between lines displaying moderate levels of resistance (`Resisto', `Regal', `Jewel') to the sweetpotato weevil [Cylas formicarius elgantulus (summers)] and weevil-susceptible lines (`Centennial', SC 1149-19, W-115), indicating a possible role of surface components in insect response. Chromatographic fractionation techniques were developed for separation of major components or groups of components. The results will allow subsequent bioassaying for the presence of an ovipositional stimulant(s) and other weevil behavior-modulating compounds and their chemical characterization.

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