Soil-applied dolomitic limestone and fertilizer affected the level of certain root and leaf ginsenosides in 4-year-old American ginseng (Panax quinquefolium L.); however, ginsenoside accumulations in the roots and in the leaves often were not similar. Root and leaf ginsenoside production tended to differ in its response to soil fertility (SF) factors and root tissue nutrient (RN) elements. Leaf ginsenoside production was more often correlated with SF factors and RN elements than that of root ginsenosides, the response of both ginsenoside sources was greater to RN than SF status. Leaf ginsenoside content was positively correlated with the SF factors and RN elements to a greater degree than that of root ginsenosides. Leaf ginsenoside production was more often affected by the same chemical element in the soil and in root tissue than that of root ginsenosides. There was no correlation between the level of any ginsenoside measured in root tissue and the same ginsenoside in leaf tissue.