Pollen Staining and High-temperature Tolerance of Bean

in Journal of the American Society for Horticultural Science
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  • 1 Western Regional Research Center, USDA/ARS, Albany, CA 94710
  • | 2 Department of Vegetable Crops, University of California, Davis, CA 95616
  • | 3 Irrigation Experiment Station, USDA/ARS, Prosser, WA 99350


Viability of pollen grains of isogenic sibling bean (Phaseolus vulgaris L.) selections of known tolerance of sensitivity to high temperatures (HT), as previously determined by pod retention and seed yield, was compared to that of a common parent bean selection and a cowpea [Vigna unguiculata (L.) Walp.] cultivar. Exposure of newly opened flowers to temperatures of 35° or 41°C reduced the viability of pollen grains in all bean selections. Pollen of all sibling selections was less affected by HT than pollen of their common parent suggesting transgressive segregation of factors for HT tolerance. At 41°, most pollen grains were destroyed in the parent bean selection and the 2 HT-sensitive siblings, whereas 44% to 55% of the pollen grains appeared to be viable in the 2 HT-tolerant siblings. Pollen viability of the HT-tolerant cowpea cultivar was not reduced by temperatures to 41°. Pollen staining indicated an interrelationship between pollen viability and tolerance to HT stress among the bean selections. The technique described has the potential for rapid selection of HT-tolerant genotypes in hybrid populations.

Contributor Notes

Received for publication 26 Nov. 1984. Reference to a company and/or produced name by the USDA is only for purposes of information and does not imply approval or recommendation of the product to the exclusion of others which may also be suitable. The cost of publishing this paper was defrayed in part by the payment of page charges. Under postal regulations, this paper therefore must be hereby marked advertisement solely to indicate this fact.