In Vitro Culture and Plant Formation from Grape Cultivars with Abortive Ovules and Seeds

in Journal of the American Society for Horticultural Science
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  • 1 Department of Fruit Breeding and Genetics, A.R.O., The Volcani Center, P.O.B. 6, Bet Dagan, Israel

Abstract

Normal embryos and seedling plants were obtained from abortive ovules and seeds of seedless grape cultivars ‘Perlette’, ‘Flame Seedless’ and ‘Sultanina’. Plant development was accompanied by callus formation only in ‘Perlette’. The best medium was Nitsch’s with the addition of 10-5m IAA and 10-6m GA3. Excision and culturing of ‘Flame Seedless’ ovules 49 days after anthesis gave higher germination percentages and more viable plants than excision and culturing at anthesis plus 28. Selfed ‘Perlette’ and ‘Perlette’ × ‘Flame Seedless’ ovules cultured at 52 days gave 11%, and the reciprocal cross over 16% well developed seedlings. The significance of the findings for breeding stenospermocarpic grapes is discussed.

Contributor Notes

Received for publication 10 May 1984. Contribution from Volcani Center, Agricultural Research Organization, Bet Dagan, Israel, No. 988-E, 1984 series. This research was supported by a grant (338-80) from the US-Israel Binational Agricultural Research and Development Fund (BARD). The authors gratefully acknowledge the computer and statistical analysis assistance by A. Gnizi. The cost of publishing this paper was defrayed in part by the payment of page charges. Under postal regulations, this paper therefore must be hereby marked advertisement solely to indicate this fact.